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Dominant interfering alleles define a role for c-Myb in T-cell development.

The transcription activator c-Myb is expressed at high levels in immature thymocytes and during T-cell activation and may be a regulator of T-cell differentiation. To investigate the role of c-Myb in T-cell development, we generated transgenic mice in which two dominant interfering Myb alleles, one a competitive inhibitor of DNA binding, and the other, an active repressor comprising the Myb DNA-binding domain linked to the Drosophila Engrailed transcription repressor domain, were expressed from early times onward in T cells. Both alleles partially blocked thymopoiesis and inhibited proliferation of mature T cells. The Myb-En chimera was the more efficient repressor and might serve as an archetype for the manufacture of other dominant interfering transcription factor alleles.

Pubmed ID: 7926766

Authors

  • Badiani P
  • Corbella P
  • Kioussis D
  • Marvel J
  • Weston K

Journal

Genes & development

Publication Data

April 1, 1994

Associated Grants

None

Mesh Terms

  • Alleles
  • Animals
  • Base Sequence
  • Cell Differentiation
  • Cell Division
  • DNA-Binding Proteins
  • Drosophila
  • Gene Expression Regulation, Developmental
  • Genes, Dominant
  • Homeodomain Proteins
  • Insect Hormones
  • Lymphocyte Activation
  • Mice
  • Mice, Transgenic
  • Molecular Sequence Data
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-myb
  • Recombinant Fusion Proteins
  • Repressor Proteins
  • Sequence Deletion
  • Spleen
  • T-Lymphocytes
  • Thymus Gland
  • Transcription Factors
  • Transcriptional Activation