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Dominant interfering alleles define a role for c-Myb in T-cell development.

Genes & development | Apr 1, 1994

The transcription activator c-Myb is expressed at high levels in immature thymocytes and during T-cell activation and may be a regulator of T-cell differentiation. To investigate the role of c-Myb in T-cell development, we generated transgenic mice in which two dominant interfering Myb alleles, one a competitive inhibitor of DNA binding, and the other, an active repressor comprising the Myb DNA-binding domain linked to the Drosophila Engrailed transcription repressor domain, were expressed from early times onward in T cells. Both alleles partially blocked thymopoiesis and inhibited proliferation of mature T cells. The Myb-En chimera was the more efficient repressor and might serve as an archetype for the manufacture of other dominant interfering transcription factor alleles.

Pubmed ID: 7926766 RIS Download

Mesh terms: Alleles | Animals | Base Sequence | Cell Differentiation | Cell Division | DNA-Binding Proteins | Drosophila | Gene Expression Regulation, Developmental | Genes, Dominant | Homeodomain Proteins | Insect Hormones | Lymphocyte Activation | Mice | Mice, Transgenic | Molecular Sequence Data | Proto-Oncogene Proteins | Proto-Oncogene Proteins c-myb | Recombinant Fusion Proteins | Repressor Proteins | Sequence Deletion | Spleen | T-Lymphocytes | Thymus Gland | Transcription Factors | Transcriptional Activation

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Associated grants


Mouse Genome Informatics (Data, Gene Annotation)

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