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Interaction between the components of the interferon gamma receptor complex.

Interferon gamma (IFN-gamma) signals through a multimeric receptor complex consisting of two different chains: the IFN-gamma receptor binding subunit (IFN-gamma R, IFN-gamma R1), and a transmembrane accessory factor (AF-1, IFN-gamma R2) necessary for signal transduction. Using cell lines expressing different cloned components of the IFN-gamma receptor complex, we examined the function of the receptor components in signal transduction upon IFN-gamma treatment. A specific IFN-gamma R2:IFN-gamma cross-linked complex was observed in cells expressing both IFN-gamma R1 and IFN-gamma R2 indicating that IFN-gamma R2 (AF-1) interacts with IFN-gamma and is closely associated with IFN-gamma R1. We show that the intracellular domain of IFN-gamma R2 is necessary for signaling. Cells coexpressing IFN-gamma R1 and truncated IFN-gamma R2, lacking the COOH-terminal 51 amino acids (residues 286-337), or cells expressing IFN-gamma R1 alone were unresponsive to IFN-gamma treatment as measured by MHC class I antigen induction. Jak1, Jak2, and Stat1 alpha were activated, and IFN-gamma R1 was phosphorylated only in cells expressing both IFN-gamma R1 and IFN-gamma R2. Jak2 kinase was shown to associate with the intracellular domain of the IFN-gamma R2.

Pubmed ID: 7673114

Authors

  • Kotenko SV
  • Izotova LS
  • Pollack BP
  • Mariano TM
  • Donnelly RJ
  • Muthukumaran G
  • Cook JR
  • Garotta G
  • Silvennoinen O
  • Ihle JN

Journal

The Journal of biological chemistry

Publication Data

September 8, 1995

Associated Grants

  • Agency: NHLBI NIH HHS, Id: P01 HL53749
  • Agency: NCI NIH HHS, Id: P30 CA21765
  • Agency: NCI NIH HHS, Id: R01 CA46465

Mesh Terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • CHO Cells
  • Cell Line
  • Cricetinae
  • DNA-Binding Proteins
  • Humans
  • Hybrid Cells
  • Janus Kinase 1
  • Janus Kinase 2
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides
  • Phosphorylation
  • Protein Binding
  • Protein-Tyrosine Kinases
  • Proto-Oncogene Proteins
  • Receptors, Interferon
  • STAT1 Transcription Factor
  • Signal Transduction
  • Trans-Activators