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Interaction between the components of the interferon gamma receptor complex.

Interferon gamma (IFN-gamma) signals through a multimeric receptor complex consisting of two different chains: the IFN-gamma receptor binding subunit (IFN-gamma R, IFN-gamma R1), and a transmembrane accessory factor (AF-1, IFN-gamma R2) necessary for signal transduction. Using cell lines expressing different cloned components of the IFN-gamma receptor complex, we examined the function of the receptor components in signal transduction upon IFN-gamma treatment. A specific IFN-gamma R2:IFN-gamma cross-linked complex was observed in cells expressing both IFN-gamma R1 and IFN-gamma R2 indicating that IFN-gamma R2 (AF-1) interacts with IFN-gamma and is closely associated with IFN-gamma R1. We show that the intracellular domain of IFN-gamma R2 is necessary for signaling. Cells coexpressing IFN-gamma R1 and truncated IFN-gamma R2, lacking the COOH-terminal 51 amino acids (residues 286-337), or cells expressing IFN-gamma R1 alone were unresponsive to IFN-gamma treatment as measured by MHC class I antigen induction. Jak1, Jak2, and Stat1 alpha were activated, and IFN-gamma R1 was phosphorylated only in cells expressing both IFN-gamma R1 and IFN-gamma R2. Jak2 kinase was shown to associate with the intracellular domain of the IFN-gamma R2.

Pubmed ID: 7673114 RIS Download

Mesh terms: Amino Acid Sequence | Animals | Base Sequence | CHO Cells | Cell Line | Cricetinae | DNA-Binding Proteins | Humans | Hybrid Cells | Janus Kinase 1 | Janus Kinase 2 | Molecular Sequence Data | Oligodeoxyribonucleotides | Phosphorylation | Protein Binding | Protein-Tyrosine Kinases | Proto-Oncogene Proteins | Receptors, Interferon | STAT1 Transcription Factor | Signal Transduction | Trans-Activators

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Associated grants

  • Agency: NHLBI NIH HHS, Id: P01 HL53749
  • Agency: NCI NIH HHS, Id: P30 CA21765
  • Agency: NCI NIH HHS, Id: R01 CA46465

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