p53 modulation of TFIIH-associated nucleotide excision repair activity.
p53 has pleiotropic functions including control of genomic plasticity and integrity. Here we report that p53 can bind to several transcription factor IIH-associated factors, including transcription-repair factors, XPD (Rad3) and XPB, as well as CSB involved in strand-specific DNA repair, via its C-terminal domain. We also found that wild-type, but not Arg273His mutant p53 inhibits XPD (Rad3) and XPB DNA helicase activities. Moreover, repair of UV-induced dimers is slower in Li-Fraumeni syndrome cells (heterozygote p53 mutant) than in normal human cells. Our findings indicate that p53 may play a direct role in modulating nucleotide excision repair pathways.
Pubmed ID: 7663514 RIS Download
Adenosine Triphosphatases | Amino Acid Sequence | Chromosome Mapping | Cockayne Syndrome | DNA Helicases | DNA Repair | DNA Repair Enzymes | DNA-Binding Proteins | Enzyme Activation | Humans | Li-Fraumeni Syndrome | Models, Molecular | Molecular Sequence Data | Nucleotides | Protein Structure, Secondary | Proteins | Saccharomyces cerevisiae Proteins | Transcription Factor TFIIH | Transcription Factors | Transcription Factors, TFII | Tumor Cells, Cultured | Tumor Suppressor Protein p53 | Xeroderma Pigmentosum | Xeroderma Pigmentosum Group D Protein