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Inducible gene targeting in mice.

Science (New York, N.Y.) | Sep 8, 1995

A method of gene targeting that allows the inducible inactivation of a target gene in mice is presented. The method uses an interferon-responsive promoter to control the expression of Cre recombinase. Here, Cre was used to delete a segment of the DNA polymerase beta gene flanked by IoxP recombinase recognition sites. Deletion was complete in liver and nearly complete in lymphocytes within a few days, whereas partial deletion was obtained in other tissues. This method can be used for the inducible inactivation of any other gene in vivo.

Pubmed ID: 7660125 RIS Download

Mesh terms: Animals | Crosses, Genetic | DNA Nucleotidyltransferases | DNA Polymerase I | Female | GTP-Binding Proteins | Gene Targeting | Genetic Vectors | Integrases | Interferon-alpha | Interferon-beta | Liver | Male | Mice | Mice, Inbred C57BL | Mice, Inbred CBA | Mice, Transgenic | Myxovirus Resistance Proteins | Poly I-C | Promoter Regions, Genetic | Proteins | Recombination, Genetic | Sequence Deletion | Viral Proteins