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Two microtubule-associated proteins required for anaphase spindle movement in Saccharomyces cerevisiae.

In many eucaryotic cells, the midzone of the mitotic spindle forms a distinct structure containing a specific set of proteins. We have isolated ASE1, a gene encoding a component of the Saccharomyces cerevisiae spindle midzone. Strains lacking both ASE1 and BIK1, which encodes an S. cerevisiae microtubule-associated protein, are inviable. The analysis of the phenotype of a bik1 ase1 conditional double mutant suggests that BIK1 and ASE1 are not required for the assembly of a bipolar spindle, but are essential for anaphase spindle elongation. The steady-state levels of Ase1p are regulated in a manner that is consistent with a function during anaphase: they are low in G1, accumulate to maximal levels after S phase and then drop as cells exit mitosis. Components of the spindle midzone may therefore be required in vivo for anaphase spindle movement. Additionally, anaphase spindle movement may depend on a dedicated set of genes whose expression is induced at G2/M.

Pubmed ID: 7559759


  • Pellman D
  • Bagget M
  • Tu YH
  • Fink GR
  • Tu H


The Journal of cell biology

Publication Data

September 6, 1995

Associated Grants

  • Agency: NIGMS NIH HHS, Id: GM-35010
  • Agency: NIGMS NIH HHS, Id: GM-40266

Mesh Terms

  • Anaphase
  • Base Sequence
  • Cell Division
  • Fungal Proteins
  • Microscopy, Electron
  • Microtubule-Associated Proteins
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Saccharomyces cerevisiae