Golgi cisternae regrew in a cell-free system from mitotic Golgi fragments incubated with buffer alone. Pretreatment with NEM or salt washing inhibited regrowth, but this could be restored either by p97, an NSF-like ATPase, or by NSF together with SNAPs and p115, a vesicle docking protein. The morphology of cisternae regrown with p97 and NSF-SNAPs-p115 differed, suggesting that they play distinct roles in rebuilding Golgi cisternae after mitosis.
Pubmed ID: 7553851 RIS Download
Mesh terms: Adenosine Triphosphatases | Animals | Carrier Proteins | Golgi Apparatus | Intracellular Membranes | Membrane Proteins | Microscopy, Electron | Mitosis | Molecular Weight | N-Ethylmaleimide-Sensitive Proteins | Rats | Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins | Vesicular Transport Proteins | Xenopus
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