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Targeted disruption of the murine VCAM1 gene: essential role of VCAM-1 in chorioallantoic fusion and placentation.

Vascular cell adhesion molecule-1 (VCAM-1) is expressed on vascular endothelium in a variety of inflammatory conditions and mediates leukocyte recruitment from blood into tissues. In this study we report a novel role for VCAM-1 in the formation of the umbilical cord and placenta during development. The murine VCAM1 gene was disrupted by targeted homologous recombination, and a distinct phenotype was found in VCAM-1-deficient embryos. At 8.5 days of gestation, the allantois failed to fuse to the chorion, resulting in abnormal placental development and embryonic death within 1-3 days. In addition, a role for VCAM-1 in early placental formation after chorioallantoic fusion was observed. In a minority of VCAM-1-deficient embryos, the allantois was able to fuse with the chorion, but the allantoic mesoderm was abnormally distributed over the chorionic surface. A small number of VCAM-1-deficient embryos survived, presumably by circumventing the placentation defects. They became viable and fertile adult mice with lack of VCAM-1 expression, normal organs, and an elevated number of circulating blood mononuclear leukocytes.

Pubmed ID: 7530222


  • Gurtner GC
  • Davis V
  • Li H
  • McCoy MJ
  • Sharpe A
  • Cybulsky MI


Genes & development

Publication Data

January 1, 1995

Associated Grants

  • Agency: NHLBI NIH HHS, Id: HL 36028
  • Agency: NHLBI NIH HHS, Id: HL 45563

Mesh Terms

  • Allantois
  • Animals
  • Base Sequence
  • Cell Adhesion Molecules
  • Chorion
  • Female
  • Heart Defects, Congenital
  • Immunohistochemistry
  • Integrin alpha4
  • Integrins
  • Leukocyte Count
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Molecular Sequence Data
  • Placenta
  • Placentation
  • Pregnancy
  • Tissue Distribution
  • Umbilical Cord
  • Vascular Cell Adhesion Molecule-1