Literature search services are currently unavailable. During our hosting provider's UPS upgrade we experienced a hardware failure and are currently working to resolve the issue.

Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Transgenic mice harboring SV40 T-antigen genes develop characteristic brain tumors.

A high percentage of transgenic mice developing from eggs microinjected with plasmids containing the SV40 early region genes and a metallothionein fusion gene develop tumors within the choroid plexus. A line of mice has been established in which nearly every affected animal succumbs to this brain tumor. Thymic hypertrophy and kidney pathology are also observed in some mice. SV40 T-antigen mRNA and protein are readily detected in affected tissues; however, SV40 T-antigen gene expression is barely detectable in unaffected tissues or in susceptible tissues prior to overt pathology, suggesting that tumorigenesis depends upon activation of the SV40 genes. Comparison of DNA from tumor tissue (or cell lines derived from tumors) with DNA from unaffected tissues reveals structural rearrangements as well as changes in DNA methylation of the foreign DNA. The SV40 genes are frequently amplified in tumor tissue, which further indicates that their expression is intimately involved in tumorigenesis in transgenic mice.

Pubmed ID: 6327063


  • Brinster RL
  • Chen HY
  • Messing A
  • van Dyke T
  • Levine AJ
  • Palmiter RD



Publication Data

June 23, 1984

Associated Grants

  • Agency: NICHD NIH HHS, Id: R01 HD017321

Mesh Terms

  • Animals
  • Antigens, Polyomavirus Transforming
  • Base Sequence
  • Brain Neoplasms
  • Crosses, Genetic
  • DNA Restriction Enzymes
  • Female
  • Genes
  • Genes, Viral
  • Male
  • Mice
  • Nucleic Acid Hybridization
  • Oncogenes
  • Pedigree
  • Plasmids
  • RNA, Messenger
  • Simian virus 40
  • Viral Proteins