Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Three different fibronectin mRNAs arise by alternative splicing within the coding region.

Cell | Dec 14, 1983

http://www.ncbi.nlm.nih.gov/pubmed/6317187

We report the isolation of cDNA clones for fibronectin from a rat liver library prepared in the expression vector, lambda gt11. Restriction mapping and DNA sequencing of these clones establish the sequence of the C-terminal 35% of rat fibronectin, covering the cell-, heparin-, and fibrin-binding domains. The cell- and heparin-binding regions have homologous repeating sequences. Based on the sequence data and S1 nuclease mapping, we conclude that there are at least three different fibronectin mRNAs in rat liver which differ in coding potential. The three RNAs appear to arise by alternative splicing within the coding region and are probably all encoded by a single gene. The implications of these results for the structure and function of fibronectin and the differences between various types of fibronectin are discussed.

Pubmed ID: 6317187 RIS Download

Mesh terms: Amino Acid Sequence | Animals | Bacteriophage lambda | Base Sequence | Cloning, Molecular | DNA | DNA Restriction Enzymes | Fibronectins | Genes | Heparin | Liver | Molecular Weight | RNA Splicing | RNA, Messenger | Rats

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.