The antiviral activity of host factor apolipoprotein B mRNA editing enzyme catalytic polypeptide-like 3G (APOBEC3G, A3G) and its degradation mediated by human immunodeficiency virus type 1 (HIV-1) Vif protein are important topics. Although accumulating evidence indicates the importance of deubiquitination enzymes (DUBs) in innate immunity, it is unknown if they participate in A3G stability. Here, we found that USP49 directly interacts with A3G and efficiently removes ubiquitin, consequently increasing A3G protein expression and significantly enhancing its anti-HIV-1 activity. Unexpectedly, A3G degradation was also mediated by a Vif- and cullin-ring-independent pathway, which was effectively counteracted by USP49. Furthermore, clinical data suggested that USP49 is correlated with A3G protein expression and hypermutations in Vif-positive proviruses, and inversely with the intact provirus ratio in the HIV-1 latent reservoir. Our studies demonstrated a mechanism to effectively stabilize A3G expression, which could comprise a target to control HIV-1 infection and eradicate the latent reservoir.
Pubmed ID: 31397674 RIS Download
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View all literature mentionsThis polyclonal targets Goat anti-Mouse IgG H&L (DyLight® 594) secondary antibody
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View all literature mentionsThis polyclonal targets ubiquitin
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View all literature mentionsCell line HEK293T is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HeLa is a Cancer cell line with a species of origin Homo sapiens
View all literature mentionsWeb application for the analysis and detection of APOBEC-induced hypermutations. The first sequence in the input alignment will be used as the reference sequence, and each of the other sequences will be used as a query sequence.
View all literature mentionsTHIS RESOURCE IS NO LONGER IN SERVICE. Documented on September 28,2023. Software application which can analyze basic Western blot data and export them for use in spreadsheet programs. Users can create standardized or custom lab reports and can share them with colleagues. Image Studio Lite has been discontinued and replaced with Empiria Studio Software.
View all literature mentionsSoftware for single-cell flow cytometry analysis. Its functions include management, display, manipulation, analysis and publication of the data stream produced by flow and mass cytometers.
View all literature mentionsStatistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsThis unknown targets ANTI-FLAG(R) M2 Affinity Gel
View all literature mentionsThis unknown targets EZview(TM) Red HA Affinity Gel
View all literature mentionsThis polyclonal secondary targets IgG
View all literature mentionsThis polyclonal targets Goat anti-Mouse IgG H&L (DyLight® 594) secondary antibody
View all literature mentionsThis polyclonal targets IgG
View all literature mentionsThis polyclonal targets Goat polyclonal Secondary to Mouse IgG - H&L (DyLight® 488) pre-adsorbed
View all literature mentionsThis polyclonal targets ubiquitin
View all literature mentionsThis monoclonal targets USP18 (D4E7) Rabbit mAb
View all literature mentionsThis monoclonal targets GFP
View all literature mentionsThis monoclonal targets APOBEC3G antibody [mAbcam75560]
View all literature mentionsThis polyclonal targets USP49
View all literature mentionsThis monoclonal targets Beta Actin
View all literature mentionsThis monoclonal targets anti-HA-tag
View all literature mentionsThis polyclonal targets GAPDH
View all literature mentionsThis polyclonal targets DDDDK-Tag (ECS/Enterokinase Cleavage Site)
View all literature mentions