Searching across hundreds of databases
Emerging evidence indicates the role of amino acid metabolism in sleep regulation. Here we demonstrate sleep-promoting effects of dietary threonine (SPET) in Drosophila. Dietary threonine markedly increased daily sleep amount and decreased the latency to sleep onset in a dose-dependent manner. High levels of synaptic GABA or pharmacological activation of metabotropic GABA receptors (GABAB-R) suppressed SPET. By contrast, synaptic blockade of GABAergic neurons or transgenic depletion of GABAB-R in the ellipsoid body R2 neurons enhanced sleep drive non-additively with SPET. Dietary threonine reduced GABA levels, weakened metabotropic GABA responses in R2 neurons, and ameliorated memory deficits in plasticity mutants. Moreover, genetic elevation of neuronal threonine levels was sufficient for facilitating sleep onset. Taken together, these data define threonine as a physiologically relevant, sleep-promoting molecule that may intimately link neuronal metabolism of amino acids to GABAergic control of sleep drive via the neuronal substrate of sleep homeostasis.
Pubmed ID: 31313987 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
A national mouse monoclonal antibody generating resource for biochemical and immunohistochemical applications in mammalian brain. NeuroMabs are generated from mice immunized with synthetic and recombinant immunogens corresponding to components of the neuronal proteome as predicted from genomic and other large-scale cloning efforts. Comprehensive biochemical and immunohistochemical analyses of human, primate and non-primate mammalian brain are incorporated into the initial NeuroMab screening procedure. This yields a subset of mouse mAbs that are optimized for use in brain (i.e. NeuroMabs): for immunocytochemical-based imaging studies of protein localization in adult, developing and pathological brain samples, for biochemical analyses of subunit composition and post-translational modifications of native brain proteins, and for proteomic analyses of native brain protein networks. The NeuroMab facility was initially funded with a five-year U24 cooperative grant from NINDS and NIMH. The initial goal of the facility for this funding period is to generate a library of novel NeuroMabs against neuronal proteins, initially focusing on membrane proteins (receptors/channels/transporters), synaptic proteins, other neuronal signaling molecules, and proteins with established links to disease states. The scope of the facility was expanded with supplements from the NIH Blueprint for Neuroscience Research to include neurodevelopmental targets, the NIH Roadmap for Medical Research to include epigenetics targets, and NIH Office of Rare Diseases Research to include rare disease targets. These NeuroMabs will then be produced on a large scale and made available to the neuroscience research community on an inexpensive basis as tissue culture supernatants or purified immunoglobulin by Antibodies Inc. The UC Davis/NIH NeuroMab Facility makes NeuroMabs available directly to end users and is unable to accommodate sales to distributors for third party distribution. Note, NeuroMab antibodies are now offered through antibodiesinc.
View all literature mentionsTHIS RESOURCE IS NO LONGER IN SERVICE. Documented on May 5,2022.Tool that predicts interactions between transcription factors and their regulated genes from binding motifs. Understanding vertebrate development requires unraveling the cis-regulatory architecture of gene regulation. PRISM provides accurate genome-wide computational predictions of transcription factor binding sites for the human and mouse genomes, and integrates the predictions with GREAT to provide functional biological context. Together, accurate computational binding site prediction and GREAT produce for each transcription factor: 1. putative binding sites, 2. putative target genes, 3. putative biological roles of the transcription factor, and 4. putative cis-regulatory elements through which the factor regulates each target in each functional role.
View all literature mentionsCommercial vendor and service provider of laboratory reagents and antibodies. Supplier of scientific instrumentation, reagents and consumables, and software services.
View all literature mentionsThis polyclonal targets IgG (H+L)
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis unknown targets GABA
View all literature mentionsThis polyclonal targets Tyrosine Hydroxylase
View all literature mentionsDrosophila melanogaster with name P{KK108036}VIE-260B from Flybase.
View all literature mentionsDrosophila melanogaster with name P{KK109166}VIE-260B from Flybase.
View all literature mentionsOpen source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{GD699}v1785 from Flybase.
View all literature mentionsSoftware application with data analysis tools and spreadsheet templates to track and visualize data. It is used to manage and process data.
View all literature mentionsDrosophila melanogaster with name PBac{fTRG00804.sfGFP-TVPTBF}VK00002 from Flybase.
View all literature mentionsUser interface software for Carl Zeiss light microscopy imaging systems. ZEN is the universal user interface you will see on every imaging system from ZEISS. After selecting fluorophore, ZEN applies the necessary settings to collect and organize data.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{GD699}v1784 from Flybase.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{GD578}v50176/TM3 from Flybase.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{GD5538}v15838 from Flybase.
View all literature mentionsProgramming language for all operating systems that lets users work more quickly and integrate their systems more effectively. Often compared to Tcl, Perl, Ruby, Scheme or Java. Some of its key distinguishing features include very clear and readable syntax, strong introspection capabilities, intuitive object orientation, natural expression of procedural code, full modularity, exception-based error handling, high level dynamic data types, extensive standard libraries and third party modules for virtually every task, extensions and modules easily written in C, C (or Java for Python, or .NET languages for IronPython), and embeddable within applications as a scripting interface.
View all literature mentionsDrosophila melanogaster with name P{VSH330042}attP40 from Flybase.
View all literature mentionsDrosophila melanogaster with name y[1] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.HMC03388}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMC05585}attP40 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=LexAop-CD8-GFP-2A-CD8-GFP}2; P{w[+mC]=UAS-mLexA-VP16-NFAT}H2, P{w[+mC]=lexAop-rCD2-GFP}3/TM6B, Tb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; Df(3L)BSC731/TM6C, Sb[1] cu[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=ple-GAL4.F}3 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mW.hs]=GawB}30Y from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{w[+mC]=UAS-Epac1-camps}50A from BDSC.
View all literature mentionsDrosophila melanogaster with name Rdl[1]/TM3, Sb[1] from DGGR.
View all literature mentionsDrosophila melanogaster with name Rdl[MD-RR] from DGGR.
View all literature mentionsDrosophila melanogaster with name w[*]; PBac{GAL4D,EYFP}Gabat[PL00338] P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=UAS-Clk.Delta}1 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[*] w[*]; PBac{SAstopDsRed}LL04492 P{w[+mW.hs]=FRT(w[hs])}2A P{ry[+t7.2]=neoFRT}82B P{y[+t7.7] ry[+t7.2]=Car20y}96E / TM6B, Tb[1] from DGGR.
View all literature mentionsDrosophila melanogaster with name Clk[Jrk] st[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; Df(3L)BSC557/TM6C, Sb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] per[01] w[*] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[1118]; PBac{y[+mDint]=3HPy[+]}Lkr[C003] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; Df(3L)Exel6123, P{w[+mC]=XP-U}Exel6123/TM6B, Tb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; TI{w[+mW.hs]=GAL4}Gr33a[GAL4] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=Orco-GAL4.W}11.17; TM2/TM6B, Tb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; wg[Sp-1]/CyO; P{w[+mC]=Gr5a-GAL4.8.5}2/TM3, Sb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=Gr66a-GAL4.D}2; TI{w[+mW.hs]=GAL4}Gr93a[3] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[1118]; PBac{y[+mDint]=3HPy[+]}Lk[C275] from BDSC.
View all literature mentionsDrosophila melanogaster with name P{ry[+t7.2]=lArB}rut[2080]; P{w[+mC]=UAS-rut.Z}2 from BDSC.
View all literature mentionsDrosophila melanogaster with name P{w[+mC]=Gad1-GAL4.3.098}2/CyO from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; Df(3L)BSC839/TM6C, Sb[1] cu[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name P{w[+mW.hs]=GawB}elav[C155] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; Df(3L)BSC797/TM6C, Sb[1] cu[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{y[+t7.7] w[+mC]=GMR58H05-GAL4}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[67c23]; P{w[+mC]=GSV7}GS20382 / TM3, Sb[1] Ser[1] from DGGR.
View all literature mentionsProgramming language for all operating systems that lets users work more quickly and integrate their systems more effectively. Often compared to Tcl, Perl, Ruby, Scheme or Java. Some of its key distinguishing features include very clear and readable syntax, strong introspection capabilities, intuitive object orientation, natural expression of procedural code, full modularity, exception-based error handling, high level dynamic data types, extensive standard libraries and third party modules for virtually every task, extensions and modules easily written in C, C (or Java for Python, or .NET languages for IronPython), and embeddable within applications as a scripting interface.
View all literature mentionsUser interface software for Carl Zeiss light microscopy imaging systems. ZEN is the universal user interface you will see on every imaging system from ZEISS. After selecting fluorophore, ZEN applies the necessary settings to collect and organize data.
View all literature mentionsSoftware application with data analysis tools and spreadsheet templates to track and visualize data. It is used to manage and process data.
View all literature mentionsOpen source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.
View all literature mentionsDrosophila melanogaster with name PBac{fTRG00804.sfGFP-TVPTBF}VK00002 from Flybase.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{GD5538}v15838 from Flybase.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{GD699}v1784 from Flybase.
View all literature mentionsDrosophila melanogaster with name P{KK109166}VIE-260B from Flybase.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{GD699}v1785 from Flybase.
View all literature mentionsDrosophila melanogaster with name P{KK108036}VIE-260B from Flybase.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{GD578}v50176/TM3 from Flybase.
View all literature mentionsThe SciCrunch Infrastructure was developed as a cooperative data platform to be used by diverse communities in making data more FAIR.
scicrunch.org
