Searching across hundreds of databases
mTORC1 regulates cellular growth and is activated by growth factors and by essential amino acids such as Leu. Leu enters cells via the Leu transporter LAT1-4F2hc (LAT1). Here we show that the Na+/K+/2Cl- cotransporter NKCC1 (SLC12A2), a known regulator of cell volume, is present in complex with LAT1. We further show that NKCC1 depletion or deletion enhances LAT1 activity, as well as activation of Akt and Erk, leading to activation of mTORC1 in cells, colonic organoids, and mouse colon. Moreover, NKCC1 depletion reduces intracellular Na+ concentration and cell volume (size) and mass and stimulates cell proliferation. NKCC1, therefore, suppresses mTORC1 by inhibiting its key activating signaling pathways. Importantly, by linking ion transport and cell volume regulation to mTORC1 function, NKCC1 provides a long-sought link connecting cell volume (size) to cell mass regulation.
Pubmed ID: 31067471 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
3D image analysis software to visualize, analyze and validate 3D fluorescence images from a wide range of confocal microscopy, widefield and high content screening systems. It is fully integrated for a seamless user experience.
View all literature mentionsSoftware that enables rapid tool creation by providing a robust, pluggable development framework that simplifies and unifies data file access, and performs standard proteomics and LCMS dataset computations.
View all literature mentionsThis monoclonal targets β-Actin
View all literature mentionsThis polyclonal targets Phospho-Akt (Ser473)
View all literature mentionsThis monoclonal targets Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal targets p38 MAPK
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis monoclonal targets GFP
View all literature mentionsThis monoclonal targets p44/42 MAPK (Erk1/2)
View all literature mentionsThis monoclonal targets LAMP1
View all literature mentionsThis monoclonal targets Akt
View all literature mentionsThis monoclonal targets mTOR
View all literature mentionsThis polyclonal targets Igf1r
View all literature mentionsThis monoclonal targets p70 S6 kinase alpha (C-18)
View all literature mentionsThis monoclonal targets Human VCL
View all literature mentionsThis monoclonal targets insulin Rbeta (CT-3)
View all literature mentionsThis polyclonal targets Phospho-Tuberin/TSC2 (Ser939)
View all literature mentionsThis polyclonal targets EIF4EBP1
View all literature mentionsThis monoclonal targets p38 MAPK (pT180/pY182)
View all literature mentionsThis polyclonal targets 4E-BP1
View all literature mentionsThis monoclonal targets NKCC1 (D13A9) Rabbit mAb
View all literature mentionsThis polyclonal targets Human NKCC1
View all literature mentionsThis monoclonal targets FLAG
View all literature mentionsThis monoclonal targets V5-TAG
View all literature mentionsThis monoclonal targets p70 S6 Kinase, phospho (Thr389)
View all literature mentionsThis monoclonal targets β-Actin
View all literature mentionsThe SciCrunch Infrastructure was developed as a cooperative data platform to be used by diverse communities in making data more FAIR.
scicrunch.org
