Varying pH of luminal fluid along the female reproductive tract is a physiological cue that modulates sperm motility. CatSper is a sperm-specific, pH-sensitive calcium channel essential for hyperactivated motility and male fertility. Multi-subunit CatSper channel complexes organize linear Ca2+ signaling nanodomains along the sperm tail. Here, we identify EF-hand calcium-binding domain-containing protein 9 (EFCAB9) as a bifunctional, cytoplasmic machine modulating the channel activity and the domain organization of CatSper. Knockout mice studies demonstrate that EFCAB9, in complex with the CatSper subunit, CATSPERζ, is essential for pH-dependent and Ca2+-sensitive activation of the CatSper channel. In the absence of EFCAB9, sperm motility and fertility is compromised, and the linear arrangement of the Ca2+ signaling domains is disrupted. EFCAB9 interacts directly with CATSPERζ in a Ca2+-dependent manner and dissociates at elevated pH. These observations suggest that EFCAB9 is a long-sought, intracellular, pH-dependent Ca2+ sensor that triggers changes in sperm motility.
Pubmed ID: 31056283 RIS Download
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View all literature mentionsThis polyclonal targets GFP
View all literature mentionsThis monoclonal targets PMCA4
View all literature mentionsThis monoclonal targets FLAG
View all literature mentionsThis monoclonal targets Calmodulin
View all literature mentionsThis monoclonal targets Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP
View all literature mentionsThis polyclonal targets IgG (H + L)
View all literature mentionsThis polyclonal targets Rabbit IgG (H+L)
View all literature mentionsThis monoclonal targets V5 Tag
View all literature mentionsThis monoclonal targets HA-Tag
View all literature mentionsThis monoclonal targets HA Tag
View all literature mentionsThis monoclonal targets c-Myc
View all literature mentionsThis monoclonal targets Phosphotyrosine
View all literature mentionsThis monoclonal targets MYC
View all literature mentionsMus musculus with name B6.129S4-Catsperdtm1.1Clph/J from IMSR.
View all literature mentionsCell line HEK293T is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsMus musculus with name B6.129S4-Catsper1tm1Clph/J from IMSR.
View all literature mentionsMus musculus with name B6.129S4-Catsperdtm1.1Clph/J from IMSR.
View all literature mentionsMus musculus with name B6.129S4-Catsper1tm1Clph/J from IMSR.
View all literature mentionsCell line HEK293T is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsThis monoclonal targets Mouse TrueBlot ULTRA: Anti-Mouse Ig HRP
View all literature mentionsThis polyclonal targets Rabbit IgG (H+L)
View all literature mentionsThis polyclonal targets IgG (H + L)
View all literature mentionsThis monoclonal targets V5 Tag
View all literature mentionsThis monoclonal targets HA-Tag
View all literature mentionsThis monoclonal targets c-Myc
View all literature mentionsThis monoclonal targets HA Tag
View all literature mentionsThis monoclonal targets Phosphotyrosine
View all literature mentionsThis monoclonal targets Calmodulin
View all literature mentionsThis monoclonal targets derived from the hybridoma 6-11B-1 produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with acetylated tubulin from the outer arm of Strongylocentrotus purpuratus (sea urchin).
View all literature mentionsThis monoclonal targets FLAG
View all literature mentionsThis monoclonal targets PMCA4
View all literature mentionsThis polyclonal targets GFP
View all literature mentions