FCRL5+ atypical memory B cells (atMBCs) expand in many chronic human infections, including recurrent malaria, but studies have drawn opposing conclusions about their function. Here, in mice infected with Plasmodium chabaudi, we demonstrate expansion of an antigen-specific FCRL5+ population that is distinct from previously described FCRL5+ innate-like murine subsets. Comparative analyses reveal overlapping phenotypic and transcriptomic signatures between FCRL5+ B cells from Plasmodium-infected mice and atMBCs from Plasmodium-exposed humans. In infected mice, FCRL5 is expressed on the majority of antigen-specific germinal-center-derived memory B cells (MBCs). Upon challenge, FCRL5+ MBCs rapidly give rise to antibody-producing cells expressing additional atypical markers, demonstrating functionality in vivo. Moreover, atypical markers are expressed on antigen-specific MBCs generated by immunization in both mice and humans, indicating that the atypical phenotype is not restricted to chronic settings. This study resolves conflicting interpretations of atMBC function and suggests FCRL5+ B cells as an attractive target for vaccine strategies.
Pubmed ID: 31042472 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
THIS RESOURCE IS NO LONGER IN SERVICE. Documented on May 5,2022.Tool that predicts interactions between transcription factors and their regulated genes from binding motifs. Understanding vertebrate development requires unraveling the cis-regulatory architecture of gene regulation. PRISM provides accurate genome-wide computational predictions of transcription factor binding sites for the human and mouse genomes, and integrates the predictions with GREAT to provide functional biological context. Together, accurate computational binding site prediction and GREAT produce for each transcription factor: 1. putative binding sites, 2. putative target genes, 3. putative biological roles of the transcription factor, and 4. putative cis-regulatory elements through which the factor regulates each target in each functional role.
View all literature mentionsInternational database for laboratory mouse. Data offered by The Jackson Laboratory includes information on integrated genetic, genomic, and biological data. MGI creates and maintains integrated representation of mouse genetic, genomic, expression, and phenotype data and develops reference data set and consensus data views, synthesizes comparative genomic data between mouse and other mammals, maintains set of links and collaborations with other bioinformatics resources, develops and supports analysis and data submission tools, and provides technical support for database users. Projects contributing to this resource are: Mouse Genome Database (MGD) Project, Gene Expression Database (GXD) Project, Mouse Tumor Biology (MTB) Database Project, Gene Ontology (GO) Project at MGI, and MouseCyc Project at MGI.
View all literature mentionsThis monoclonal targets IgM
View all literature mentionsThis monoclonal targets Human CD20
View all literature mentionsThis monoclonal targets CD3 epsilon
View all literature mentionsThis monoclonal targets CD73
View all literature mentionsThis monoclonal targets CD11c
View all literature mentionsThis monoclonal targets CD307e (FcRL5)
View all literature mentionsThis monoclonal targets Human CD38
View all literature mentionsThis monoclonal targets CD27
View all literature mentionsThis monoclonal targets IgD
View all literature mentionsThis monoclonal targets IgM
View all literature mentionsThis monoclonal targets GL7
View all literature mentionsThis monoclonal targets CD23
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis monoclonal targets Mouse CD45.2
View all literature mentionsThis monoclonal targets Mouse CD45.1
View all literature mentionsThis monoclonal targets IgD
View all literature mentionsThis monoclonal targets Mouse CD21 / CD35 (CR2 / CR1)
View all literature mentionsThis monoclonal targets Ki-67
View all literature mentionsThis monoclonal targets CD86
View all literature mentionsThis monoclonal targets T-bet
View all literature mentionsThis monoclonal targets CD72 a, b and d Alloantigens
View all literature mentionsThis monoclonal targets CD11b
View all literature mentionsThis monoclonal targets CD5
View all literature mentionsThis monoclonal targets Mouse CD1d (CD1.1, Ly-38)
View all literature mentionsThis monoclonal targets CD8a
View all literature mentionsThis monoclonal targets Mouse CD4 (L3T4)
View all literature mentionsThis monoclonal targets CD38
View all literature mentionsThis monoclonal targets CD138
View all literature mentionsThis monoclonal targets CD45R (B220)
View all literature mentionsMus musculus with name B6.C(Cg)-Cd79atm1(cre)Reth/EhobJ from IMSR.
View all literature mentionsMus musculus with name B6.SJL-Ptprca Pepc/BoyJ from IMSR.
View all literature mentionsSoftware package for differential gene expression analysis based on the negative binomial distribution. Used for analyzing RNA-seq data for differential analysis of count data, using shrinkage estimation for dispersions and fold changes to improve stability and interpretability of estimates.
View all literature mentionsBioinformatics resource system including web server and web service for functional annotation and enrichment analyses of gene lists. Consists of comprehensive knowledgebase and set of functional analysis tools. Includes gene centered database integrating heterogeneous gene annotation resources to facilitate high throughput gene functional analysis.
View all literature mentionsSoftware package for quantifying gene and isoform abundances from single end or paired end RNA Seq data. Accurate transcript quantification from RNA Seq data with or without reference genome. Used for accurate quantification of gene and isoform expression from RNA-Seq data.
View all literature mentionsMus musculus with name B6.129-Tbx21tm2Srnr/J from IMSR.
View all literature mentionsMus musculus with name B6.129S7-Ifngr1tm1Agt/J from IMSR.
View all literature mentionsMus musculus with name C57BL/6J from IMSR.
View all literature mentionsStatistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsOpen source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.
View all literature mentions