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Though human prostate cancer (PCa) heterogeneity can best be studied using multiple cell types isolated from clinical specimens, the difficulty of establishing cell lines from clinical tumors has hampered this approach. In this proof-of-concept study, we established a human PCa cell line from a prostatectomy surgical specimen without the need for retroviral transduction. In a previous report, we characterized the stromal cells derived from PCa specimens. Here, we characterized the epithelial cells isolated from the same tumors. Compared to the ease of establishing prostate stromal cell lines, prostatic epithelial cell lines are challenging. From three matched pairs of normal and tumor tissues, we established one new PCa cell line, HPE-15. We confirmed the origin of HPE-15 cells by short tandem repeat microsatellite polymorphism analysis. HPE-15 cells are androgen-insensitive and express marginal androgen receptor, prostate-specific antigen and prostate-specific membrane antigen proteins. HPE-15 expresses luminal epithelial markers of E-cadherin and cytokeratin 18, basal cell markers of cytokeratin 5 and p63 and neuroendocrine marker of chromogranin A. Interestingly, HPE-15 Cells exhibited no tumorigenicity in different strains of immune-deficient mice but can become tumorigenic through interaction with aggressive cancer cell types. HPE-15 cells can thus serve as an experimental model for the study of PCa progression, metastasis and tumor cell dormancy.
Pubmed ID: 31020641 RIS Download
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This monoclonal targets beta-Actin (AC-15)
View all literature mentionsThis monoclonal targets Raised against amino acids 299-315 of AR of human origin.
View all literature mentionsThis monoclonal targets E-Cadherin (32A8) Mouse mAb
View all literature mentionsThis monoclonal targets Cytokeratin 5 (RCK103)
View all literature mentionsThis monoclonal targets Human KRT18
View all literature mentionsThis monoclonal targets FoxA2/HNF3
View all literature mentionsThis polyclonal targets anti-Red Fluorescent Protein (RFP) antibody
View all literature mentionsCell line ARCaP is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line LNCaP is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line PC-3 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsThis monoclonal targets Human KRT18
View all literature mentionsThis monoclonal targets Cytokeratin 5 (RCK103)
View all literature mentionsThis polyclonal targets anti-Red Fluorescent Protein (RFP) antibody
View all literature mentionsThis monoclonal targets FoxA2/HNF3
View all literature mentionsThis monoclonal targets E-Cadherin (32A8) Mouse mAb
View all literature mentionsThis monoclonal targets beta-Actin (AC-15)
View all literature mentionsThis monoclonal targets Raised against amino acids 299-315 of AR of human origin.
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