In ectotherms, increased ambient temperature requires the organism to consume substantial amounts of energy to sustain a higher metabolic rate, prevent cellular damage, and respond to heat stress. Here, we identify a heat-inducible apolipoprotein required for thermal acclimation in Drosophila. Neuropeptide-like precursor 2 (Nplp2) is an abundant hemolymphatic protein thought to be a neuropeptide. In contrast, we show that Nplp2 contributes to lipid transport, functioning as an exchangeable apolipoprotein. More precisely, Nplp2-deficient flies accumulate lipids in their gut, have reduced fat stores, and display a dyslipoproteinemia, showing that Nplp2 is required for dietary lipid assimilation. Importantly, Nplp2 is induced upon thermal stress and contributes to survival upon heat stress. We propose that Nplp2 associates with lipoprotein particles under homeostatic and high energy-demand conditions to optimize fat transport and storage. Our study also shows that modulation of the lipid uptake and transport machinery is part of an integrated cytoprotective response.
Pubmed ID: 30995484 RIS Download
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View all literature mentionsDrosophila melanogaster with name y[1] w[*]; P{Act5C-GAL4-w}E1/CyO from BDSC.
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View all literature mentionsThis unknown targets Rat IgG (H+L)
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View all literature mentionsThis polyclonal targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgY (H+L)
View all literature mentionsThis monoclonal targets V5 Tag
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal targets GFP
View all literature mentionsDrosophila melanogaster with name y[1] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.HMJ21484}attP40 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{GD14739}v29349/CyO from Flybase.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; P{Act5C-GAL4-w}E1/CyO from BDSC.
View all literature mentionsThis unknown targets
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=Mhc-RFP.F3-580}2, P{w[+mC]=Mhc-GAL4.F3-580}2/SM6b from BDSC.
View all literature mentionsThis unknown targets Rat IgG (H+L)
View all literature mentionsDrosophila melanogaster with name w[1118]; P{w[+mW.hs]=GawB}c564 from BDSC.
View all literature mentionsThis monoclonal targets c-Myc clone 9E10
View all literature mentions