Obesity is a public health problem worldwide, and especially in women in reproductive age where more than one in three have obesity. Maternal obesity is associated with an increased maternal, placental, and newborn oxidative stress, which has been proposed as a central factor in vascular dysfunction in large-for-gestational-age (LGA) newborn. However, cellular and molecular mechanisms behind this effect have not been elucidated. Untreated human umbilical artery endothelial cells (HUAEC) from LGA (LGA-HUAEC) presented higher O2- levels, superoxide dismutase activity and heme oxygenase 1 messenger RNA (mRNA) levels, paralleled by reduced GSH:GSSG ratio and NRF2 mRNA levels. In response to an oxidative challenge (hydrogen peroxide), only HUAEC from LGA exhibited an enhanced Glutathione Peroxidase 1 (GPX1) expression, as well as a more efficient antioxidant machinery measured by the biosensor probe, HyPer. An open state of chromatin in the TSS region of GPX1 in LGA-HUAEC was evidenced by the DNase-HS assay. Altogether, our data indicate that LGA-HUAEC have an altered cellular and molecular antioxidant system. We propose that a chronic pro-oxidant intrauterine milieu, as evidenced in pregestational obesity, could induce a more efficient antioxidant system in fetal vascular cells, which could be maintained by epigenetic mechanism during postnatal life.
Pubmed ID: 30937903 RIS Download
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This monoclonal targets Glutathione Peroxidase 1 antibody [EPR3312]
View all literature mentionsThis monoclonal targets Superoxide Dismutase 1
View all literature mentionsThis monoclonal targets Heme Oxygenase 1
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View all literature mentionsThis monoclonal targets Superoxide Dismutase 1
View all literature mentionsThis monoclonal targets Glutathione Peroxidase 1 antibody [EPR3312]
View all literature mentionsThis monoclonal targets NOX4 antibody [UOTR1B492]
View all literature mentionsStatistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsOpen source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.
View all literature mentionsThis monoclonal targets Superoxide Dismutase 1
View all literature mentionsThis monoclonal targets Glutathione Peroxidase 1 antibody [EPR3312]
View all literature mentionsThis monoclonal targets Heme Oxygenase 1
View all literature mentionsThis monoclonal targets beta-Actin
View all literature mentionsThis monoclonal targets NOX4 antibody [UOTR1B492]
View all literature mentionsThis unknown targets Nrf2
View all literature mentions