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Small open reading frames (smORFs) encoding 'micropeptides' exhibit remarkable evolutionary complexity. Conserved peptides encoded by mille-pattes (mlpt)/polished rice (pri)/tarsal less (tal) are essential for embryo segmentation in Tribolium but, in Drosophila, function in terminal epidermal differentiation and patterning of adult legs. Here, we show that a molecular complex identified in Drosophila epidermal differentiation, comprising Mlpt peptides, ubiquitin-ligase Ubr3 and transcription factor Shavenbaby (Svb), represents an ancient developmental module required for early insect embryo patterning. We find that loss of segmentation function for this module in flies evolved concomitantly with restriction of Svb expression in early Drosophila embryos. Consistent with this observation, artificially restoring early Svb expression in flies causes segmentation defects that depend on mlpt function, demonstrating enduring potency of an ancestral developmental switch despite evolving embryonic patterning modes. These results highlight the evolutionary plasticity of conserved molecular complexes under the constraints of essential genetic networks.
Pubmed ID: 30896406 RIS Download
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Tool used to design PCR primers from DNA sequence - often in high-throughput genomics applications. It does everything from mispriming libraries to sequence quality data to the generation of internal oligos.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{w[+mC]=UAS-EGFP}5a.2 from BDSC.
View all literature mentionsThis unknown targets IgG
View all literature mentionsThis unknown targets Digoxigenin
View all literature mentionsThis monoclonal targets Ultrabithorax and abdominal-A proteins (all isoforms)
View all literature mentionsThis monoclonal targets Mouse Drosophila Wingless protein
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal targets IgG (H+L)
View all literature mentionsDrosophila melanogaster with name w[1118]; P{w[+mC]=GAL4::VP16-nos.UTR}CG6325[MVD1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; Dr[Mio]/TM3, P{w[+mC]=GAL4-twi.G}2.3, P{UAS-2xEGFP}AH2.3, Sb[1] Ser[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{ry[+t7.2]=neoFRT}82B P{w[+mC]=ovoD1-18}3R/st[1] betaTub85D[D] ss[1] e[s]/TM3, Sb[1] from DGGR.
View all literature mentionsVector graphics software to create digital graphics, illustrations, and typography for several types of media: print, web, interactive, video, and mobile.
View all literature mentionsDrosophila melanogaster with name Df(1)JA27/FM7c, P{w[+mC]=GAL4-Kr.C}DC1, P{w[+mC]=UAS-GFP.S65T}DC5, sn[+] from BDSC.
View all literature mentionsSoftware application that provides sequence editing, primer design, internet database searching, protein analysis, sequence confirmation, multiple sequence alignment, phylogenetic reconstruction, coding region analysis, agarose gel simulation and a variety of other functions.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; P{w[+mC]=nullo-GAL4.G}5.20 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=UAS-GFP.S65T}Myo31DF[T2] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; Df(3L)Ly, sens[Ly-1]/TM6B, P{w[+mW.hs]=Ubi-GFP.S65T}PAD2, Tb[1] from BDSC.
View all literature mentionsSoftware for image processing, analysis, and editing. The software includes features such as touch capabilities, a customizable toolbar, 2D and 3D image merging, and Cloud access and options.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mW.hs]=GawB}ptc[559.1] from BDSC.
View all literature mentionsDrosophila melanogaster with name P{w[+mC]=ovoD1-18}P4.1, P{ry[+t7.2]=hsFLP}12, y[1] w[1118] sn[3] P{ry[+t7.2]=neoFRT}19A/C(1)DX, y[1] f[1] from BDSC.
View all literature mentionsStatistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsA web interface to the Primer3 primer design program as an enhanced alternative for the CGI- scripts that come with Primer3.
View all literature mentionsDrosophila melanogaster with name w[1118]; Df(3L)Ly, sens[Ly-1]/TM6B, P{w[+mW.hs]=Ubi-GFP.S65T}PAD2, Tb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{w[+mC]=GAL4::VP16-nos.UTR}CG6325[MVD1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{w[+mC]=GAL4::VP16-nos.UTR}CG6325[MVD1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{ry[+t7.2]=neoFRT}82B P{w[+mC]=ovoD1-18}3R/st[1] betaTub85D[D] ss[1] e[s]/TM3, Sb[1] from DGGR.
View all literature mentionsThis polyclonal targets IgG (H+L)
View all literature mentionsA web interface to the Primer3 primer design program as an enhanced alternative for the CGI- scripts that come with Primer3.
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis unknown targets IgG
View all literature mentionsStatistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsDrosophila melanogaster with name w[1118]; Df(3L)Ly, sens[Ly-1]/TM6B, P{w[+mW.hs]=Ubi-GFP.S65T}PAD2, Tb[1] from BDSC.
View all literature mentionsSoftware application that provides sequence editing, primer design, internet database searching, protein analysis, sequence confirmation, multiple sequence alignment, phylogenetic reconstruction, coding region analysis, agarose gel simulation and a variety of other functions.
View all literature mentionsThis unknown targets Digoxigenin
View all literature mentionsThis monoclonal targets Ultrabithorax and abdominal-A proteins (all isoforms)
View all literature mentionsDrosophila melanogaster with name w[*]; P{ry[+t7.2]=neoFRT}82B P{w[+mC]=ovoD1-18}3R/st[1] betaTub85D[D] ss[1] e[s]/TM3, Sb[1] from DGGR.
View all literature mentionsDrosophila melanogaster with name P{w[+mC]=ovoD1-18}P4.1, P{ry[+t7.2]=hsFLP}12, y[1] w[1118] sn[3] P{ry[+t7.2]=neoFRT}19A/C(1)DX, y[1] f[1] from BDSC.
View all literature mentionsThis monoclonal targets Mouse Drosophila Wingless protein
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=UAS-GFP.S65T}Myo31DF[T2] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mW.hs]=GawB}ptc[559.1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; P{w[+mC]=nullo-GAL4.G}5.20 from BDSC.
View all literature mentionsDrosophila melanogaster with name Df(1)JA27/FM7c, P{w[+mC]=GAL4-Kr.C}DC1, P{w[+mC]=UAS-GFP.S65T}DC5, sn[+] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; Dr[Mio]/TM3, P{w[+mC]=GAL4-twi.G}2.3, P{UAS-2xEGFP}AH2.3, Sb[1] Ser[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=UAS-GFP.S65T}Myo31DF[T2] from BDSC.
View all literature mentionsDrosophila melanogaster with name P{w[+mC]=ovoD1-18}P4.1, P{ry[+t7.2]=hsFLP}12, y[1] w[1118] sn[3] P{ry[+t7.2]=neoFRT}19A/C(1)DX, y[1] f[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; P{w[+mC]=nullo-GAL4.G}5.20 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mW.hs]=GawB}ptc[559.1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; Dr[Mio]/TM3, P{w[+mC]=GAL4-twi.G}2.3, P{UAS-2xEGFP}AH2.3, Sb[1] Ser[1] from BDSC.
View all literature mentionsVector graphics software to create digital graphics, illustrations, and typography for several types of media: print, web, interactive, video, and mobile.
View all literature mentionsDrosophila melanogaster with name Df(1)JA27/FM7c, P{w[+mC]=GAL4-Kr.C}DC1, P{w[+mC]=UAS-GFP.S65T}DC5, sn[+] from BDSC.
View all literature mentionsSoftware for image processing, analysis, and editing. The software includes features such as touch capabilities, a customizable toolbar, 2D and 3D image merging, and Cloud access and options.
View all literature mentionsThe SciCrunch Infrastructure was developed as a cooperative data platform to be used by diverse communities in making data more FAIR.
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