Many potential targets for CAR-T cells in solid tumors are expressed in some normal tissues, raising concern for off-tumor toxicity. Following lymphodepletion, CAR-T cells targeting the tumor-associated antigen ROR1 lysed tumors in mice but induced lethal bone marrow failure due to recognition of ROR1+ stromal cells. To improve selectivity, we engineered T cells with synthetic Notch (synNotch) receptors specific for EpCAM or B7-H3, which are expressed on ROR1+ tumor cells but not ROR1+ stromal cells. SynNotch receptors induced ROR1 CAR expression selectively within the tumor, resulting in tumor regression without toxicity when tumor cells were segregated from, but not when co-localized with, normal ROR1+ cells. This strategy, thus, permits safe targeting of tumors that are sufficiently separated from normal cells.
Pubmed ID: 30889382 RIS Download
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This monoclonal targets CD144 (VE-cadherin)
View all literature mentionsThis monoclonal targets CD135
View all literature mentionsThis monoclonal targets TER-119
View all literature mentionsThis recombinant monoclonal targets ROR1
View all literature mentionsThis monoclonal targets IFN-gamma
View all literature mentionsThis monoclonal targets CD4
View all literature mentionsThis monoclonal targets CD366
View all literature mentionsThis monoclonal targets CD45
View all literature mentionsThis monoclonal targets CD8a
View all literature mentionsThis monoclonal targets EGFR
View all literature mentionsThis monoclonal targets CD8
View all literature mentionsThis monoclonal targets CD279
View all literature mentionsThis monoclonal targets TER-119
View all literature mentionsThis monoclonal targets CD366
View all literature mentionsThis monoclonal targets CD45
View all literature mentionsThis isotype control targets Not Applicable
View all literature mentionsThis monoclonal targets TER-119
View all literature mentionsThis monoclonal targets CD279
View all literature mentionsThis monoclonal targets Ly-6A/E
View all literature mentionsThis monoclonal targets CD279
View all literature mentionsThis monoclonal targets Myc-Tag (9B11) Mouse mAb (Alexa Fluor 647 Conjugate)
View all literature mentionsThis unknown targets Ki-67
View all literature mentionsThis monoclonal targets Ly-6G Ly-6C
View all literature mentionsThis monoclonal targets IFN gamma
View all literature mentionsThis monoclonal targets CD326 (EpCAM)
View all literature mentionsThis monoclonal targets CD117
View all literature mentionsThis monoclonal targets CD8 APC-eFluor 780 120 tests
View all literature mentionsThis monoclonal targets CD8a
View all literature mentionsThis monoclonal targets CD8a
View all literature mentionsThis monoclonal targets CD45.1
View all literature mentionsThis monoclonal targets CD51
View all literature mentionsThis monoclonal targets CD45.1
View all literature mentionsThis monoclonal targets CD48
View all literature mentionsThis monoclonal targets CD45
View all literature mentionsThis monoclonal targets CD45
View all literature mentionsThis monoclonal targets CD4
View all literature mentionsThis monoclonal targets CD45.1
View all literature mentionsThis monoclonal targets mouse CD4
View all literature mentionsThis monoclonal targets CD31
View all literature mentionsThis monoclonal targets CD34
View all literature mentionsThis monoclonal targets CD19
View all literature mentionsThis monoclonal targets CD3epsilon
View all literature mentionsThis monoclonal targets CD19
View all literature mentionsThis monoclonal targets CD3e
View all literature mentionsThis monoclonal targets CD140b
View all literature mentionsThis monoclonal targets CD16/32
View all literature mentionsThis monoclonal targets CD140a
View all literature mentionsThis monoclonal targets CD150
View all literature mentionsThis monoclonal targets CD19
View all literature mentionsThis monoclonal targets CD11b
View all literature mentionsThis monoclonal targets CD45R
View all literature mentionsThis monoclonal targets CD11c
View all literature mentionsCell line 4T1 is a Cancer cell line with a species of origin Mus musculus (Mouse)
View all literature mentionsMus musculus with name B6;129S4-Ror1tm1.1Meg/J from IMSR.
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Mus musculus with name NOD.Cg-Prkdcscid Il2rg
Mus musculus with name B6.FVB-Tg(EIIa-cre)C5379Lmgd/J from IMSR.
View all literature mentionsMus musculus with name CByJ.SJL(B6)-Ptprca/J from IMSR.
View all literature mentionsMus musculus with name B6.SJL-Ptprca Pepc/BoyJ from IMSR.
View all literature mentionsMus musculus with name BALB/cByJ from IMSR.
View all literature mentionsMus musculus with name C.B6(Cg)-Rag2tm1.1Cgn/J from IMSR.
View all literature mentionsCell line SK-N-DZ is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line Plat-E is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsMus musculus with name C57BL/6J from IMSR.
View all literature mentionsCell line Raji is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line K-562 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line MDA-MB-231 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line 4T1 is a Cancer cell line with a species of origin Mus musculus (Mouse)
View all literature mentionsMus musculus with name CByJ.SJL(B6)-Ptprca/J from IMSR.
View all literature mentionsMus musculus with name B6.SJL-Ptprca Pepc/BoyJ from IMSR.
View all literature mentionsMus musculus with name C57BL/6J from IMSR.
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Mus musculus with name NOD.Cg-Prkdcscid Il2rg
Mus musculus with name B6;129S4-Ror1tm1.1Meg/J from IMSR.
View all literature mentionsMus musculus with name B6.FVB-Tg(EIIa-cre)C5379Lmgd/J from IMSR.
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