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Publication

Circulating CD56bright NK cells inversely correlate with survival of melanoma patients.

Scientific reports | 2019

The roles of NK cells in human melanoma remain only partially understood. We characterized NK cells from peripheral blood ex vivo by flow cytometry obtained from late stage (III/IV) melanoma patients. Interestingly, we found that the abundance of CD56bright NK cells negatively correlate with overall patient survival, together with distant metastases, in a multivariate cox regression analysis. The patients' CD56bright NK cells showed upregulation of CD11a, CD38 and CD95 as compared to healthy controls, pointing to an activated phenotype as well as a possible immune regulatory role in melanoma patients. After stimulation in vitro, CD56bright NK cells produced less TNFα and GMCSF in patients than controls. Furthermore, IFNγ production by the CD56bright NK cells correlated inversely with overall survival. Our results highlight that abundance and function of CD56bright NK cells are associated with melanoma patient survival, emphasizing the potential of NK cell subsets for biomarker discovery and future therapeutic targeting.

Pubmed ID: 30872676 RIS Download

Research resources used in this publication

Additional research tools detected in this publication

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Associated grants

None

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

This is a list of tools and resources that we have found mentioned in this publication.

FlowJo (tool)

RRID:SCR_008520

Software for single-cell flow cytometry analysis. Its functions include management, display, manipulation, analysis and publication of the data stream produced by flow and mass cytometers.

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BD Biosciences (tool)

RRID:SCR_013311

An Antibody supplier

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Rabbit Anti-FoxP3 Polyclonal Antibody, FITC Conjugated (antibody)

RRID:AB_11076963

This polyclonal targets Rabbit FoxP3 FITC

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Granzyme B Monoclonal Antibody (GB11), PE-Texas Red (antibody)

RRID:AB_2536540

This monoclonal targets Granzyme B

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FITC anti-human Perforin (antibody)

RRID:AB_314702

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PE anti-human IL-4 (antibody)

RRID:AB_315129

This monoclonal targets IL-4

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Mouse Anti-Human Il-22 Monoclonal antibody, Phycoerythrin Conjugated, Clone 142928 (antibody)

RRID:AB_495011

This monoclonal targets Human IL-22

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CCL4 (MIP-1 beta) Monoclonal Antibody (FL34Z3L), PerCP-eFluor™ 710, eBioscience (antibody)

RRID:AB_2573845

This monoclonal targets CCL4 (MIP-1 beta)

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APC anti-human GM-CSF (antibody)

RRID:AB_11150231

This monoclonal targets GM-CSF

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IL-13 (antibody)

RRID:AB_10642586

This monoclonal targets IL-13

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PE anti-human IL-10 (antibody)

RRID:AB_315454

This monoclonal targets IL-10

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Annexin V - Apoptosis stain (antibody)

RRID:AB_2665412

This unknown targets

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TNF beta Monoclonal Antibody (LTX-21), FITC, eBioscience (antibody)

RRID:AB_10598519

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BV510 Mouse Anti-Human CD127 (antibody)

RRID:AB_2728655

This monoclonal targets CD127

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PE/Dazzle(TM) 594 anti-human CD137 (4-1BB) (antibody)

RRID:AB_2566260

This monoclonal targets CD137

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PerCP/Cyanine5.5 anti-human CD69 (antibody)

RRID:AB_2074956

This monoclonal targets CD69

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CD3 Monoclonal Antibody (SK7), APC-eFluor™ 780, eBioscience (antibody)

RRID:AB_10717514

This monoclonal targets CD3

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PerCP/Cyanine5.5 anti-human CD336 (NKp44) (antibody)

RRID:AB_2616752

This monoclonal targets CD336

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Alexa Fluor(R) 700 anti-human CD16 (antibody)

RRID:AB_2278418

This monoclonal targets CD16

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CD152 (antibody)

RRID:AB_398615

This monoclonal targets CD152

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Brilliant Violet 421(TM) anti-human CD56 (NCAM) (antibody)

RRID:AB_11218798

This monoclonal targets CD56

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CD38 Monoclonal Antibody (HIT2), PE-Cyanine7, eBioscience (antibody)

RRID:AB_1724057

This monoclonal targets CD38

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APC anti-human CD95 (Fas) (antibody)

RRID:AB_314550

This monoclonal targets CD95

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CD159a (NKG2A)-PE-Vio770, human (antibody)

RRID:AB_2655388

This monoclonal targets CD159a (NKG2A)

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CD57 FITC (antibody)

RRID:AB_2728105

This monoclonal targets CD57

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BV510 Mouse Anti-Human CD123 (antibody)

RRID:AB_2728102

This monoclonal targets CD123

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BV510 Mouse Anti-Human CD141 (antibody)

RRID:AB_2728103

This monoclonal targets CD141

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Mouse anti-human CD158b PE (antibody)

RRID:AB_2728104

This monoclonal targets CD158b1/b2,j

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Brilliant Violet 421(TM) anti-human CD127 (IL-7Ralpha) (antibody)

RRID:AB_10960140

This monoclonal targets CD127

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Mouse anti-human CD14 Krome Orange (antibody)

RRID:AB_2728099

This monoclonal targets CD14

View all literature mentions

Mouse anti-human CD3 Pacific Blue (antibody)

RRID:AB_2728095

This monoclonal targets CD3

View all literature mentions

Mouse anti-human CD19 Krome Orange (antibody)

RRID:AB_2728101

This unknown targets CD19

View all literature mentions

Alexa Fluor(R) 700 anti-human CD8a (antibody)

RRID:AB_528885

This monoclonal targets CD8a

View all literature mentions

Nkp46 (antibody)

RRID:AB_10894195

This monoclonal targets Nkp46

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APC anti-human CD314 (NKG2D) (antibody)

RRID:AB_492962

This monoclonal targets CD314

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Mouse Anti-Human CD279 Monoclonal Antibody, PE Conjugated, Clone MIH4 (antibody)

RRID:AB_647199

This monoclonal targets CD279

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PerCP/Cyanine5.5 anti-human CD56 (NCAM) (antibody)

RRID:AB_893389

This monoclonal targets CD56

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Mouse Anti-CD11a Monoclonal Antibody, FITC Conjugated, Clone G-25.2 (antibody)

RRID:AB_400366

This monoclonal targets CD11a

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Mouse anti-human CD16 ECD (antibody)

RRID:AB_2728092

This monoclonal targets CD16

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Mouse Anti-Human CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra) Monoclonal Antibody, Pacific Blue Conjugated, Clone eBioRDR5 (antibody)

RRID:AB_657602

This monoclonal targets Human CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra)

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CD4 PE-Cy™7 Clone SK3 (antibody)

RRID:AB_2783789

This monoclonal targets CD4

View all literature mentions

CD25 PE Clone 2A3 (antibody)

RRID:AB_2783790

This monoclonal targets CD25

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CD3 PerCP Clone SK7 (antibody)

RRID:AB_2783791

This monoclonal targets CD3

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IL-5 (antibody)

RRID:AB_398548

This monoclonal targets IL-5

View all literature mentions

PerCP/Cyanine5.5 anti-human TNF-alpha (antibody)

RRID:AB_2204081

This monoclonal targets TNF-alpha

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CCL3 (MIP-1α) Antibody, anti-human, PE-Vio® 770, REAfinity™ (antibody)

RRID:AB_2651378

This recombinant monoclonal targets CCL3 (MIP-1α)

View all literature mentions

Mouse anti-human CD19 Krome Orange (antibody)

RRID:AB_2728101

This unknown targets CD19

View all literature mentions

Mouse anti-human CD14 Krome Orange (antibody)

RRID:AB_2728099

This monoclonal targets CD14

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Mouse Anti-Human CD279 Monoclonal Antibody, PE Conjugated, Clone MIH4 (antibody)

RRID:AB_647199

This monoclonal targets CD279

View all literature mentions

Mouse anti-human CD3 Pacific Blue (antibody)

RRID:AB_2728095

This monoclonal targets CD3

View all literature mentions

Alexa Fluor(R) 700 anti-human CD8a (antibody)

RRID:AB_528885

This monoclonal targets CD8a

View all literature mentions

Mouse Anti-CD11a Monoclonal Antibody, FITC Conjugated, Clone G-25.2 (antibody)

RRID:AB_400366

This monoclonal targets CD11a

View all literature mentions

BV510 Mouse Anti-Human CD123 (antibody)

RRID:AB_2728102

This monoclonal targets CD123

View all literature mentions

Brilliant Violet 421(TM) anti-human CD127 (IL-7Ralpha) (antibody)

RRID:AB_10960140

This monoclonal targets CD127

View all literature mentions

Mouse anti-human CD158b PE (antibody)

RRID:AB_2728104

This monoclonal targets CD158b1/b2,j

View all literature mentions

Mouse anti-human CD16 ECD (antibody)

RRID:AB_2728092

This monoclonal targets CD16

View all literature mentions

APC anti-human CD314 (NKG2D) (antibody)

RRID:AB_492962

This monoclonal targets CD314

View all literature mentions

PerCP/Cyanine5.5 anti-human CD56 (NCAM) (antibody)

RRID:AB_893389

This monoclonal targets CD56

View all literature mentions

Nkp46 (antibody)

RRID:AB_10894195

This monoclonal targets Nkp46

View all literature mentions

Mouse Anti-Human CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra) Monoclonal Antibody, Pacific Blue Conjugated, Clone eBioRDR5 (antibody)

RRID:AB_657602

This monoclonal targets Human CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra)

View all literature mentions

Rabbit Anti-FoxP3 Polyclonal Antibody, FITC Conjugated (antibody)

RRID:AB_11076963

This polyclonal targets Rabbit FoxP3 FITC

View all literature mentions

CD25 PE Clone 2A3 (antibody)

RRID:AB_2783790

This monoclonal targets CD25

View all literature mentions

CD4 PE-Cy™7 Clone SK3 (antibody)

RRID:AB_2783789

This monoclonal targets CD4

View all literature mentions

CD3 PerCP Clone SK7 (antibody)

RRID:AB_2783791

This monoclonal targets CD3

View all literature mentions

IL-5 (antibody)

RRID:AB_398548

This monoclonal targets IL-5

View all literature mentions

CCL3 (MIP-1α) Antibody, anti-human, PE-Vio® 770, REAfinity™ (antibody)

RRID:AB_2651378

This recombinant monoclonal targets CCL3 (MIP-1α)

View all literature mentions

PerCP/Cyanine5.5 anti-human TNF-alpha (antibody)

RRID:AB_2204081

This monoclonal targets TNF-alpha

View all literature mentions

Granzyme B Monoclonal Antibody (GB11), PE-Texas Red (antibody)

RRID:AB_2536540

This monoclonal targets Granzyme B

View all literature mentions

PE anti-human IL-4 (antibody)

RRID:AB_315129

This monoclonal targets IL-4

View all literature mentions

FITC anti-human Perforin (antibody)

RRID:AB_314702

This monoclonal targets Perforin

View all literature mentions

IL-13 (antibody)

RRID:AB_10642586

This monoclonal targets IL-13

View all literature mentions

CCL4 (MIP-1 beta) Monoclonal Antibody (FL34Z3L), PerCP-eFluor™ 710, eBioscience (antibody)

RRID:AB_2573845

This monoclonal targets CCL4 (MIP-1 beta)

View all literature mentions

Mouse Anti-Human Il-22 Monoclonal antibody, Phycoerythrin Conjugated, Clone 142928 (antibody)

RRID:AB_495011

This monoclonal targets Human IL-22

View all literature mentions

APC anti-human GM-CSF (antibody)

RRID:AB_11150231

This monoclonal targets GM-CSF

View all literature mentions

PE anti-human IL-10 (antibody)

RRID:AB_315454

This monoclonal targets IL-10

View all literature mentions

TNF beta Monoclonal Antibody (LTX-21), FITC, eBioscience (antibody)

RRID:AB_10598519

This monoclonal targets TNF beta

View all literature mentions

Annexin V - Apoptosis stain (antibody)

RRID:AB_2665412

This unknown targets

View all literature mentions

BV510 Mouse Anti-Human CD127 (antibody)

RRID:AB_2728655

This monoclonal targets CD127

View all literature mentions

PerCP/Cyanine5.5 anti-human CD336 (NKp44) (antibody)

RRID:AB_2616752

This monoclonal targets CD336

View all literature mentions

PerCP/Cyanine5.5 anti-human CD69 (antibody)

RRID:AB_2074956

This monoclonal targets CD69

View all literature mentions

PE/Dazzle(TM) 594 anti-human CD137 (4-1BB) (antibody)

RRID:AB_2566260

This monoclonal targets CD137

View all literature mentions

CD3 Monoclonal Antibody (SK7), APC-eFluor™ 780, eBioscience (antibody)

RRID:AB_10717514

This monoclonal targets CD3

View all literature mentions

Alexa Fluor(R) 700 anti-human CD16 (antibody)

RRID:AB_2278418

This monoclonal targets CD16

View all literature mentions

Brilliant Violet 421(TM) anti-human CD56 (NCAM) (antibody)

RRID:AB_11218798

This monoclonal targets CD56

View all literature mentions

CD152 (antibody)

RRID:AB_398615

This monoclonal targets CD152

View all literature mentions

APC anti-human CD95 (Fas) (antibody)

RRID:AB_314550

This monoclonal targets CD95

View all literature mentions

CD38 Monoclonal Antibody (HIT2), PE-Cyanine7, eBioscience (antibody)

RRID:AB_1724057

This monoclonal targets CD38

View all literature mentions

CD57 FITC (antibody)

RRID:AB_2728105

This monoclonal targets CD57

View all literature mentions

CD159a (NKG2A)-PE-Vio770, human (antibody)

RRID:AB_2655388

This monoclonal targets CD159a (NKG2A)

View all literature mentions

BV510 Mouse Anti-Human CD141 (antibody)

RRID:AB_2728103

This monoclonal targets CD141

View all literature mentions

Mouse anti-human CD19 Krome Orange (antibody)

RRID:AB_2728101

This unknown targets CD19

View all literature mentions

Mouse anti-human CD14 Krome Orange (antibody)

RRID:AB_2728099

This monoclonal targets CD14

View all literature mentions

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