Efficiency of reprogramming of human cells into induced pluripotent stem cells (iPSCs) has remained low. We report that individual adult human CD49f+ long-term hematopoietic stem cells (LT-HSCs) can be reprogrammed into iPSCs at close to 50% efficiency using Sendai virus transduction. This exquisite sensitivity to reprogramming is specific to LT-HSCs, since it progressively decreases in committed progenitors. LT-HSC reprogramming can follow multiple paths and is most efficient when transduction is performed after the cells have exited G0. Sequencing of 75 paired skin fibroblasts/LT-HSC samples collected from nine individuals revealed that LT-HSCs contain a lower load of somatic single-nucleotide variants (SNVs) and indels than skin fibroblasts and accumulate about 12 SNVs/year. Mutation analysis revealed that LT-HSCs and fibroblasts have very different somatic mutation signatures and that somatic mutations in iPSCs generally exist prior to reprogramming. LT-HSCs may become the preferred cell source for the production of clinical-grade iPSCs.
Pubmed ID: 30840883 RIS Download
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Multi paradigm numerical computing environment and fourth generation programming language developed by MathWorks. Allows matrix manipulations, plotting of functions and data, implementation of algorithms, creation of user interfaces, and interfacing with programs written in other languages, including C, C++, Java, Fortran and Python. Used to explore and visualize ideas and collaborate across disciplines including signal and image processing, communications, control systems, and computational finance.
View all literature mentionsNetwork of ftp and web servers around world that store identical, up to date, versions of code and documentation for R. Package archive network for R programming language.
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View all literature mentionsA software package to analyze next-generation resequencing data. The toolkit offers a wide variety of tools, with a primary focus on variant discovery and genotyping as well as strong emphasis on data quality assurance. Its robust architecture, powerful processing engine and high-performance computing features make it capable of taking on projects of any size. This software library makes writing efficient analysis tools using next-generation sequencing data very easy, and second it's a suite of tools for working with human medical resequencing projects such as 1000 Genomes and The Cancer Genome Atlas. These tools include things like a depth of coverage analyzers, a quality score recalibrator, a SNP/indel caller and a local realigner. (entry from Genetic Analysis Software)
View all literature mentionsDatabase as central repository for both single base nucleotide substitutions and short deletion and insertion polymorphisms. Distinguishes report of how to assay SNP from use of that SNP with individuals and populations. This separation simplifies some issues of data representation. However, these initial reports describing how to assay SNP will often be accompanied by SNP experiments measuring allele occurrence in individuals and populations. Community can contribute to this resource.
View all literature mentionsPrivately held company that develops and produces antibodies, ELISA kits, ChIP kits, proteomic kits, and other related reagents used to study cell signaling pathways that impact human health.
View all literature mentionsIntegrated structural variant prediction software that can detect deletions, tandem duplications, inversions and translocations at single-nucleotide resolution in short-read massively parallel sequencing data. It uses paired-ends and split-reads to sensitively and accurately delineate genomic rearrangements throughout genome.
View all literature mentionsSoftware R package provides set of flexible functions to evaluate and visualize multitude of mutational patterns in base substitution catalogues of e.g. healthy samples, tumour samples, or DNA-repair deficient cells.
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View all literature mentionsThis polyclonal targets Ki67 antibody - Proliferation Marker
View all literature mentionsThis monoclonal targets TRA-1-60 (Podocalyxin)
View all literature mentionsThis monoclonal targets CD33
View all literature mentionsThis monoclonal targets CD45.1
View all literature mentionsThis monoclonal targets TRA-1-81 (Podocalyxin)
View all literature mentionsThis monoclonal targets CD49f (Integrin alpha 6)
View all literature mentionsThis monoclonal targets CD45
View all literature mentionsThis monoclonal targets CD45RA
View all literature mentionsThis monoclonal targets CD90 (Thy-1)
View all literature mentionsThis monoclonal targets CD38
View all literature mentionsThis monoclonal targets CD34
View all literature mentionsThis polyclonal targets Ki67 antibody - Proliferation Marker
View all literature mentionsThis monoclonal targets TRA-1-60 (Podocalyxin)
View all literature mentionsThis monoclonal targets TRA-1-81 (Podocalyxin)
View all literature mentionsThis monoclonal targets CD19
View all literature mentionsThis monoclonal targets CD33
View all literature mentionsThis monoclonal targets CD45.1
View all literature mentionsThis monoclonal targets CD45
View all literature mentionsThis monoclonal targets CD49f (Integrin alpha 6)
View all literature mentionsThis monoclonal targets CD45RA
View all literature mentionsThis monoclonal targets CD90 (Thy-1)
View all literature mentionsThis monoclonal targets CD38
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