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PP2A-B56 is a serine/threonine phosphatase complex that regulates several major mitotic processes, including sister chromatid cohesion, kinetochore-microtubule attachment and the spindle assembly checkpoint. We show here that these key functions are divided between different B56 isoforms that localise to either the centromere or kinetochore. The centromeric isoforms rely on a specific interaction with Sgo2, whereas the kinetochore isoforms bind preferentially to BubR1 and other proteins containing an LxxIxE motif. In addition to these selective binding partners, Sgo1 helps to anchor PP2A-B56 at both locations: it collaborates with BubR1 to maintain B56 at the kinetochore and it helps to preserve the Sgo2/B56 complex at the centromere. A series of chimaeras were generated to map the critical region in B56 down to a small C-terminal loop that regulates the key interactions and defines B56 localisation. Together, this study describes how different PP2A-B56 complexes utilise isoform-specific interactions to control distinct processes during mitosis.
Pubmed ID: 30829571 RIS Download
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View all literature mentionsTHIS RESOURCE IS NO LONGER IN SERVICE. Documented on May 5,2022.Tool that predicts interactions between transcription factors and their regulated genes from binding motifs. Understanding vertebrate development requires unraveling the cis-regulatory architecture of gene regulation. PRISM provides accurate genome-wide computational predictions of transcription factor binding sites for the human and mouse genomes, and integrates the predictions with GREAT to provide functional biological context. Together, accurate computational binding site prediction and GREAT produce for each transcription factor: 1. putative binding sites, 2. putative target genes, 3. putative biological roles of the transcription factor, and 4. putative cis-regulatory elements through which the factor regulates each target in each functional role.
View all literature mentionsAn Antibody supplier and subset of ThermoFisher Scientific which provides fluorescence reagents for various experiments and methods.
View all literature mentionsCell line HeLa is a Cancer cell line with a species of origin Homo sapiens
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View all literature mentionsThis monoclonal targets PP2A C subunit clone 1D6
View all literature mentionsThis monoclonal targets PP2A A Subunit
View all literature mentionsThis polyclonal targets BubR1
View all literature mentionsThis monoclonal targets PP2A-B56-gamma (A-11)
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View all literature mentionsThis polyclonal targets Actin
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View all literature mentionsThis monoclonal targets Axin1 (C76H11) Rabbit mAb
View all literature mentionsThis polyclonal targets BubR1
View all literature mentionsThis monoclonal targets PP2A C subunit clone 1D6
View all literature mentionsThis monoclonal targets B56 alpha
View all literature mentionsThis monoclonal targets FLAG
View all literature mentionsThis monoclonal targets PP2A-B56-gamma (A-11)
View all literature mentionsThis monoclonal targets PP2A-B56-delta (H-11)
View all literature mentionsThis monoclonal targets BubR1
View all literature mentionsThis polyclonal targets Human CASC5
View all literature mentionsThis monoclonal targets VSV Glycoprotein
View all literature mentionsThis unknown targets Goat Mouse IgG (H L)-HRP Conjugate
View all literature mentionsThis unknown targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis unknown targets Chicken IgG (H+L)
View all literature mentionsThis monoclonal targets α-tubulin
View all literature mentionsThis polyclonal targets CDCA2 antibody produced in rabbit
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