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Zika virus is a pathogen that poses serious consequences, including congenital microcephaly. Although many viruses reprogram host cell metabolism, whether Zika virus alters cellular metabolism and the functional consequences of Zika-induced metabolic changes remain unknown. Here, we show that Zika virus infection differentially reprograms glucose metabolism in human versus C6/36 mosquito cells by increasing glucose use in the tricarboxylic acid cycle in human cells versus increasing glucose use in the pentose phosphate pathway in mosquito cells. Infection of human cells selectively depletes nucleotide triphosphate levels, leading to elevated AMP/ATP ratios, AMP-activated protein kinase (AMPK) phosphorylation, and caspase-mediated cell death. AMPK is also phosphorylated in Zika virus-infected mouse brain. Inhibiting AMPK in human cells decreases Zika virus-mediated cell death, whereas activating AMPK in mosquito cells promotes Zika virus-mediated cell death. These findings suggest that the differential metabolic reprogramming during Zika virus infection of human versus mosquito cells determines whether cell death occurs.
Pubmed ID: 30827860 RIS Download
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This monoclonal targets ACC
View all literature mentionsThis polyclonal targets Zika virus Envelope protein
View all literature mentionsThis polyclonal targets Acetyl-CoA Carboxylase 1
View all literature mentionsThis polyclonal targets Caspase-3
View all literature mentionsThis monoclonal targets AMPK-alpha, phospho (Thr172)
View all literature mentionsThis monoclonal targets beta-actin
View all literature mentionsThis monoclonal targets alpha-Tubulin antibody produced in mouse
View all literature mentionsThis monoclonal targets Casp3
View all literature mentionsCell line ATC-15 is a Spontaneously immortalized cell line with a species of origin Aedes albopictus (Asian tiger mosquito)
View all literature mentionsCell line C6/36 is a Spontaneously immortalized cell line with a species of origin Aedes albopictus (Asian tiger mosquito)
View all literature mentionsCell line ATC-10 is a Spontaneously immortalized cell line with a species of origin Aedes aegypti (Yellowfever mosquito)
View all literature mentionsCell line Vero is a Spontaneously immortalized cell line with a species of origin Chlorocebus sabaeus
View all literature mentionsCell line HFF-1 is a Finite cell line with a species of origin Homo sapiens (Human)
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