Searching across hundreds of databases
In the mammalian testes, such as in rats, the directional alignment of polarized elongating/elongated spermatids, in particular step 17-19 spermatids, across the plane of seminiferous epithelium resembles planar cell polarity (PCP) found in hair cells of the cochlea. It is obvious that spermatid PCP is necessary to support the simultaneous development of maximal number of elongating/elongated spermatids to sustain the daily production of > 50 million sperm per adult rat. Studies have shown that the testis indeed expresses multiple PCP proteins necessary to support spermatid PCP. Herein, using physiological and biochemical assays, and morphological analysis, and with the technique of RNA interference (RNAi) to knockdown PCP protein Dishevelled (Dvl) 1 (Dvl1), Dvl2, Dvl3, or Dvl1/2/3, Dvl proteins, in particular Dvl3, it was shown that Dvl3 played a crucial role of support Sertoli cell tight junction (TJ)-permeability barrier function through changes in the organization of actin- and microtubule (MT)-based cytoskeletons. More important, an in vivo knockdown of Dvl1/2/3 in the testis, defects of spermatid polarity were remarkably noted across the seminiferous epithelium, concomitant with defects of spermatid adhesion and spermatid transport, leading to considerably defects in spermatogenesis. More important, Dvl1/2/3 triple knockdown in the testis also impeded the organization of actin- and MT-based cytoskeletons owing to disruptive spatial expression of actin- and MT-regulatory proteins. In summary, PCP Dishevelled proteins, in particular, Dvl3 is a regulator of Sertoli cell blood-testis barrier (BTB) and also spermatid PCP function through its effects on the actin- and MT-based cytoskeletons in Sertoli cells.
Pubmed ID: 30808893 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
This monoclonal targets frizzled-3 (C-1)
View all literature mentionsThis polyclonal targets Vangl2 (N-13)
View all literature mentionsThis monoclonal targets Dvl-1 (3F12)
View all literature mentionsThis polyclonal targets Dishevelled-3
View all literature mentionsThis monoclonal targets DVL3 antibody produced in mouse
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis unknown targets Rabbit IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal targets Goat IgG
View all literature mentionsThis unknown targets Mouse IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis monoclonal targets GAPDH antibody [6C5]
View all literature mentionsThis unknown targets Occludin
View all literature mentionsThis monoclonal targets β-Actin
View all literature mentionsThis unknown targets Claudin 11
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal targets Human Detyrosinated alpha Tubulin
View all literature mentionsThis monoclonal targets Tubulin, Tyrosine
View all literature mentionsThis polyclonal targets beta Tubulin
View all literature mentionsThis monoclonal targets Acetylated alpha Tubulin
View all literature mentionsThis monoclonal targets alpha Tubulin antibody [DM1A] - Loading Control
View all literature mentionsThis monoclonal targets Vimentin (V9)
View all literature mentionsThis polyclonal targets MARK4
View all literature mentionsThis monoclonal targets ARP3
View all literature mentionsThis polyclonal targets CLIP1
View all literature mentionsThis monoclonal targets N-cadherin
View all literature mentionsThis unknown targets beta Catenin
View all literature mentionsThis polyclonal targets CDH2
View all literature mentionsThis polyclonal targets CTNNB1
View all literature mentionsThis unknown targets ZO-1
View all literature mentionsThe SciCrunch Infrastructure was developed as a cooperative data platform to be used by diverse communities in making data more FAIR.
scicrunch.org
