Addgene (tool)

RRID:SCR_002037

Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.

Fiji (tool)

RRID:SCR_002285

Software package as distribution of ImageJ and ImageJ2 together with Java, Java3D and plugins organized into coherent menu structure. Used to assist research in life sciences.

Vaa3D (tool)

RRID:SCR_002609

A handy, fast, and versatile 3D/4D/5D Image Visualization & Analysis System for Bioimages & Surface Objects. Vaa3D is a cross-platform (Mac, Linux, and Windows) tool for visualizing large-scale (gigabytes, and 64-bit data) 3D/4D/5D image stacks and various surface data. It is also a container of powerful modules for 3D image analysis (cell segmentation, neuron tracing, brain registration, annotation, quantitative measurement and statistics, etc) and data management. Vaa3D is very easy to be extended via a powerful plugin interface. For example, many ITK tools are being converted to Vaa3D Plugins. Vaa3D-Neuron is built upon Vaa3D to make 3D neuron reconstruction much easier. In a recent Nature Biotechnology paper (2010, 28(4), pp.348-353) about Vaa3D and Vaa3D-Neuron, an order of magnitude of performance improvement (both reconstruction accuracy and speed) was achieved compared to other tools.

BestGene (tool)

RRID:SCR_012605

We provide high quality Drosophila transgenic service to both research institutions and companies. We offer you partial to full service ranging from DNA preparation, embryo microinjection, screening for white+, yellow+, and/or GFP/RFP phenotypes, to balancing crosses. Most importantly, the cost of our Drosophila embryo injection services is more reasonable compared to that of others. With a large number of facilities and the highly experienced staff, we are able to initiate the process immediately upon receiving your sample. Our friendly web-based database allows you to track your sample status, service history and more.

w[*]; P{w[+m*]=Mef2-GAL4.247}3 (tool)

RRID:BDSC_50742

Drosophila melanogaster with name w[*]; P{w[+m*]=Mef2-GAL4.247}3 from BDSC.

Myocyte enhancer factor 2 antibody (antibody)

RRID:AB_2568604

This polyclonal targets Mef2

GFP antibody (antibody)

RRID:AB_10710875

This polyclonal targets GFP antibody

w[1118]; P{w[+mW.hs]=GawB}VGlut[OK371] (organism)

RRID:BDSC_26160

Drosophila melanogaster with name w[1118]; P{w[+mW.hs]=GawB}VGlut[OK371] from BDSC.

P{w[+mC]=alphaTub84B(FRT.GAL80)}1, w[*]; Bl[1]/CyO; TM2/TM6B, Tb[1] (organism)

RRID:BDSC_38879

Drosophila melanogaster with name P{w[+mC]=alphaTub84B(FRT.GAL80)}1, w[*]; Bl[1]/CyO; TM2/TM6B, Tb[1] from BDSC.

y[1] w[*]; Mi{Trojan-GAL4.1}dpr7[MI05719-TG4.1]/In(4)ci[D], ci[D] pan[ciD] (organism)

RRID:BDSC_78385

Drosophila melanogaster with name y[1] w[*]; Mi{Trojan-GAL4.1}dpr7[MI05719-TG4.1]/In(4)ci[D], ci[D] pan[ciD] from BDSC.

w[1118]; P{y[+t7.7] w[+mC]=GMR13C09-GAL4}attP2 (organism)

RRID:BDSC_48555

Drosophila melanogaster with name w[1118]; P{y[+t7.7] w[+mC]=GMR13C09-GAL4}attP2 from BDSC.

pBS-KS-attB2-SA(0)-T2A-QF2-Hsp70 (plasmid)

RRID:Addgene_62944

y[1] w[*]; Mi{Trojan-GAL4.1}dpr17[MI08707-TG4.1]/TM3, Sb[1] Ser[1] (organism)

RRID:BDSC_76200

Drosophila melanogaster with name y[1] w[*]; Mi{Trojan-GAL4.1}dpr17[MI08707-TG4.1]/TM3, Sb[1] Ser[1] from BDSC.

pBS-KS-attB2-SA(1)-T2A-QF2-Hsp70 (plasmid)

RRID:Addgene_62945

y[1] w[*]; wg[Sp-1]/CyO, P{Wee-P.ph0}Bacc[Wee-P20]; P{y[+t7.7] w[+mC]=10XQUAS-6XmCherry-HA}attP2 (organism)

RRID:BDSC_52270

Drosophila melanogaster with name y[1] w[*]; wg[Sp-1]/CyO, P{Wee-P.ph0}Bacc[Wee-P20]; P{y[+t7.7] w[+mC]=10XQUAS-6XmCherry-HA}attP2 from BDSC.

y[1] w[*]; PBac{y[+mDint2] w[+mC]=10XQUAS-6XmCherry-HA}VK00018/CyO, P{Wee-P.ph0}Bacc[Wee-P20]; Dr[1]/TM6C, Sb[1] Tb[1] (organism)

RRID:BDSC_52269

Drosophila melanogaster with name y[1] w[*]; PBac{y[+mDint2] w[+mC]=10XQUAS-6XmCherry-HA}VK00018/CyO, P{Wee-P.ph0}Bacc[Wee-P20]; Dr[1]/TM6C, Sb[1] Tb[1] from BDSC.

w[*]; P{w[+mC]=tubP-GAL80[ts]}10; TM2/TM6B, Tb[1] (organism)

RRID:BDSC_7108

Drosophila melanogaster with name w[*]; P{w[+mC]=tubP-GAL80[ts]}10; TM2/TM6B, Tb[1] from BDSC.

y[1] w[*]; wg[Sp-1]/CyO, P{Wee-P.ph0}Bacc[Wee-P20]; P{y[+t7.7] w[+mC]=20XUAS-6XGFP}attP2 (organism)

RRID:BDSC_52262

Drosophila melanogaster with name y[1] w[*]; wg[Sp-1]/CyO, P{Wee-P.ph0}Bacc[Wee-P20]; P{y[+t7.7] w[+mC]=20XUAS-6XGFP}attP2 from BDSC.

y[1] w[*]; betaTub60D[Pin-1]/CyO; P{w[+mC] RFP[mCh.3xP3.cPa]=Mef2-QF2.L}3/TM6B, Tb[1] (organism)

RRID:BDSC_66469

Drosophila melanogaster with name y[1] w[*]; betaTub60D[Pin-1]/CyO; P{w[+mC] RFP[mCh.3xP3.cPa]=Mef2-QF2.L}3/TM6B, Tb[1] from BDSC.

pJFRC28-10XUAS-IVS-GFP-p10 (plasmid)

RRID:Addgene_36431

w[1118]; P{y[+t7.7] w[+mC]=QUAS-DSCP-FLPo.2G}attP2 (organism)

RRID:BDSC_30008

Drosophila melanogaster with name w[1118]; P{y[+t7.7] w[+mC]=QUAS-DSCP-FLPo.2G}attP2 from BDSC.

y[1] w[*]; PBac{y[+mDint2] w[+mC]=13XLexAop2-6XGFP}VK00018/CyO, P{Wee-P.ph0}Bacc[Wee-P20]; Dr[1]/TM6C, Sb[1] Tb[1] (organism)

RRID:BDSC_52265

Drosophila melanogaster with name y[1] w[*]; PBac{y[+mDint2] w[+mC]=13XLexAop2-6XGFP}VK00018/CyO, P{Wee-P.ph0}Bacc[Wee-P20]; Dr[1]/TM6C, Sb[1] Tb[1] from BDSC.

w[1118]; PBac{w[+mC]=IT.GAL4}hkb[0016-G4] (organism)

RRID:BDSC_62578

Drosophila melanogaster with name w[1118]; PBac{w[+mC]=IT.GAL4}hkb[0016-G4] from BDSC.

w[*]; P{w[+mC]=alphaTub84B(FRT.GAL80)}2/CyO; TM2/TM6B, Tb[1] (organism)

RRID:BDSC_38880

Drosophila melanogaster with name w[*]; P{w[+mC]=alphaTub84B(FRT.GAL80)}2/CyO; TM2/TM6B, Tb[1] from BDSC.

y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS01671}attP40 (organism)

RRID:BDSC_38227

Drosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS01671}attP40 from BDSC.

y[1] w[*]; PBac{y[+mDint2] w[+mC]=20XUAS-6XGFP}VK00018/CyO, P{Wee-P.ph0}Bacc[Wee-P20] (organism)

RRID:BDSC_52261

Drosophila melanogaster with name y[1] w[*]; PBac{y[+mDint2] w[+mC]=20XUAS-6XGFP}VK00018/CyO, P{Wee-P.ph0}Bacc[Wee-P20] from BDSC.

P{ry[+t7.2]=neoFRT}42D (organism)

RRID:BDSC_1802

Drosophila melanogaster with name P{ry[+t7.2]=neoFRT}42D from BDSC.

y[1] w[*]; wg[Sp-1]/CyO, P{Wee-P.ph0}Bacc[Wee-P20]; P{y[+t7.7] w[+mC]=20XUAS-6XmCherry-HA}attP2 (organism)

RRID:BDSC_52268

Drosophila melanogaster with name y[1] w[*]; wg[Sp-1]/CyO, P{Wee-P.ph0}Bacc[Wee-P20]; P{y[+t7.7] w[+mC]=20XUAS-6XmCherry-HA}attP2 from BDSC.

y[1] w[*] Mi{PT-GFSTF.1}DIP-alpha[MI02031-GFSTF.1] (organism)

RRID:BDSC_60523

Drosophila melanogaster with name y[1] w[*] Mi{PT-GFSTF.1}DIP-alpha[MI02031-GFSTF.1] from BDSC.

y[1] w[*]; Mi{PT-GFSTF.1}dpr10[MI03557-GFSTF.1]/TM6C, Sb[1] Tb[1] (organism)

RRID:BDSC_59807

Drosophila melanogaster with name y[1] w[*]; Mi{PT-GFSTF.1}dpr10[MI03557-GFSTF.1]/TM6C, Sb[1] Tb[1] from BDSC.

w[*]; Mi{Trojan-QF2.2}VGlut[MI04979-TQF2.2]/CyO; P{w[+mC]=QUAS-mCD8-GFP.P}5B (organism)

RRID:BDSC_60315

Drosophila melanogaster with name w[*]; Mi{Trojan-QF2.2}VGlut[MI04979-TQF2.2]/CyO; P{w[+mC]=QUAS-mCD8-GFP.P}5B from BDSC.

w[1118]; P{y[+t7.7] w[+mC]=GMR10C12-GAL4}attP2 (organism)

RRID:BDSC_47841

Drosophila melanogaster with name w[1118]; P{y[+t7.7] w[+mC]=GMR10C12-GAL4}attP2 from BDSC.

y[1] w[*]; PBac{y[+mDint2] w[+mC]=10XQUAS-6XGFP}VK00018/CyO, P{Wee-P.ph0}Bacc[Wee-P20] (organism)

RRID:BDSC_52264

Drosophila melanogaster with name y[1] w[*]; PBac{y[+mDint2] w[+mC]=10XQUAS-6XGFP}VK00018/CyO, P{Wee-P.ph0}Bacc[Wee-P20] from BDSC.

w[*]; Mi{Trojan-QF2.2}VGlut[MI04979-TQF2.2]/CyO; P{w[+mC]=QUAS-mCD8-GFP.P}5B (organism)

RRID:BDSC_60315

Drosophila melanogaster with name w[*]; Mi{Trojan-QF2.2}VGlut[MI04979-TQF2.2]/CyO; P{w[+mC]=QUAS-mCD8-GFP.P}5B from BDSC.

y[1] w[*]; betaTub60D[Pin-1]/CyO; P{w[+mC] RFP[mCh.3xP3.cPa]=Mef2-QF2.L}3/TM6B, Tb[1] (organism)

RRID:BDSC_66469

Drosophila melanogaster with name y[1] w[*]; betaTub60D[Pin-1]/CyO; P{w[+mC] RFP[mCh.3xP3.cPa]=Mef2-QF2.L}3/TM6B, Tb[1] from BDSC.

y[1] w[*]; wg[Sp-1]/CyO, P{Wee-P.ph0}Bacc[Wee-P20]; P{y[+t7.7] w[+mC]=20XUAS-6XmCherry-HA}attP2 (organism)

RRID:BDSC_52268

Drosophila melanogaster with name y[1] w[*]; wg[Sp-1]/CyO, P{Wee-P.ph0}Bacc[Wee-P20]; P{y[+t7.7] w[+mC]=20XUAS-6XmCherry-HA}attP2 from BDSC.

w[1118]; P{y[+t7.7] w[+mC]=QUAS-DSCP-FLPo.2G}attP2 (organism)

RRID:BDSC_30008

Drosophila melanogaster with name w[1118]; P{y[+t7.7] w[+mC]=QUAS-DSCP-FLPo.2G}attP2 from BDSC.

y[1] w[*]; PBac{y[+mDint2] w[+mC]=10XQUAS-6XmCherry-HA}VK00018/CyO, P{Wee-P.ph0}Bacc[Wee-P20]; Dr[1]/TM6C, Sb[1] Tb[1] (organism)

RRID:BDSC_52269

Drosophila melanogaster with name y[1] w[*]; PBac{y[+mDint2] w[+mC]=10XQUAS-6XmCherry-HA}VK00018/CyO, P{Wee-P.ph0}Bacc[Wee-P20]; Dr[1]/TM6C, Sb[1] Tb[1] from BDSC.

y[1] w[*]; PBac{y[+mDint2] w[+mC]=20XUAS-6XGFP}VK00018/CyO, P{Wee-P.ph0}Bacc[Wee-P20] (organism)

RRID:BDSC_52261

Drosophila melanogaster with name y[1] w[*]; PBac{y[+mDint2] w[+mC]=20XUAS-6XGFP}VK00018/CyO, P{Wee-P.ph0}Bacc[Wee-P20] from BDSC.

y[1] w[*]; PBac{y[+mDint2] w[+mC]=10XQUAS-6XGFP}VK00018/CyO, P{Wee-P.ph0}Bacc[Wee-P20] (organism)

RRID:BDSC_52264

Drosophila melanogaster with name y[1] w[*]; PBac{y[+mDint2] w[+mC]=10XQUAS-6XGFP}VK00018/CyO, P{Wee-P.ph0}Bacc[Wee-P20] from BDSC.

y[1] w[*]; wg[Sp-1]/CyO, P{Wee-P.ph0}Bacc[Wee-P20]; P{y[+t7.7] w[+mC]=10XQUAS-6XmCherry-HA}attP2 (organism)

RRID:BDSC_52270

Drosophila melanogaster with name y[1] w[*]; wg[Sp-1]/CyO, P{Wee-P.ph0}Bacc[Wee-P20]; P{y[+t7.7] w[+mC]=10XQUAS-6XmCherry-HA}attP2 from BDSC.

y[1] w[*]; PBac{y[+mDint2] w[+mC]=13XLexAop2-6XGFP}VK00018/CyO, P{Wee-P.ph0}Bacc[Wee-P20]; Dr[1]/TM6C, Sb[1] Tb[1] (organism)

RRID:BDSC_52265

Drosophila melanogaster with name y[1] w[*]; PBac{y[+mDint2] w[+mC]=13XLexAop2-6XGFP}VK00018/CyO, P{Wee-P.ph0}Bacc[Wee-P20]; Dr[1]/TM6C, Sb[1] Tb[1] from BDSC.

y[1] w[*]; wg[Sp-1]/CyO, P{Wee-P.ph0}Bacc[Wee-P20]; P{y[+t7.7] w[+mC]=20XUAS-6XGFP}attP2 (organism)

RRID:BDSC_52262

Drosophila melanogaster with name y[1] w[*]; wg[Sp-1]/CyO, P{Wee-P.ph0}Bacc[Wee-P20]; P{y[+t7.7] w[+mC]=20XUAS-6XGFP}attP2 from BDSC.

w[1118]; P{w[+mW.hs]=GawB}VGlut[OK371] (organism)

RRID:BDSC_26160

Drosophila melanogaster with name w[1118]; P{w[+mW.hs]=GawB}VGlut[OK371] from BDSC.

w[1118]; P{y[+t7.7] w[+mC]=GMR10C12-GAL4}attP2 (organism)

RRID:BDSC_47841

Drosophila melanogaster with name w[1118]; P{y[+t7.7] w[+mC]=GMR10C12-GAL4}attP2 from BDSC.

pJFRC28-10XUAS-IVS-GFP-p10 (plasmid)

RRID:Addgene_36431

y[1] w[*]; Mi{PT-GFSTF.1}dpr10[MI03557-GFSTF.1]/TM6C, Sb[1] Tb[1] (organism)

RRID:BDSC_59807

Drosophila melanogaster with name y[1] w[*]; Mi{PT-GFSTF.1}dpr10[MI03557-GFSTF.1]/TM6C, Sb[1] Tb[1] from BDSC.

w[1118]; PBac{w[+mC]=IT.GAL4}hkb[0016-G4] (organism)

RRID:BDSC_62578

Drosophila melanogaster with name w[1118]; PBac{w[+mC]=IT.GAL4}hkb[0016-G4] from BDSC.

y[1] w[*] Mi{PT-GFSTF.1}DIP-alpha[MI02031-GFSTF.1] (organism)

RRID:BDSC_60523

Drosophila melanogaster with name y[1] w[*] Mi{PT-GFSTF.1}DIP-alpha[MI02031-GFSTF.1] from BDSC.

w[1118]; P{y[+t7.7] w[+mC]=GMR13C09-GAL4}attP2 (organism)

RRID:BDSC_48555

Drosophila melanogaster with name w[1118]; P{y[+t7.7] w[+mC]=GMR13C09-GAL4}attP2 from BDSC.

w[*]; P{w[+mC]=tubP-GAL80[ts]}10; TM2/TM6B, Tb[1] (organism)

RRID:BDSC_7108

Drosophila melanogaster with name w[*]; P{w[+mC]=tubP-GAL80[ts]}10; TM2/TM6B, Tb[1] from BDSC.

y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS01671}attP40 (organism)

RRID:BDSC_38227

Drosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS01671}attP40 from BDSC.

y[1] w[*]; Mi{Trojan-GAL4.1}dpr17[MI08707-TG4.1]/TM3, Sb[1] Ser[1] (organism)

RRID:BDSC_76200

Drosophila melanogaster with name y[1] w[*]; Mi{Trojan-GAL4.1}dpr17[MI08707-TG4.1]/TM3, Sb[1] Ser[1] from BDSC.

w[*]; P{w[+mC]=alphaTub84B(FRT.GAL80)}2/CyO; TM2/TM6B, Tb[1] (organism)

RRID:BDSC_38880

Drosophila melanogaster with name w[*]; P{w[+mC]=alphaTub84B(FRT.GAL80)}2/CyO; TM2/TM6B, Tb[1] from BDSC.

y[1] w[*]; Mi{Trojan-GAL4.1}dpr7[MI05719-TG4.1]/In(4)ci[D], ci[D] pan[ciD] (organism)

RRID:BDSC_78385

Drosophila melanogaster with name y[1] w[*]; Mi{Trojan-GAL4.1}dpr7[MI05719-TG4.1]/In(4)ci[D], ci[D] pan[ciD] from BDSC.

P{w[+mC]=alphaTub84B(FRT.GAL80)}1, w[*]; Bl[1]/CyO; TM2/TM6B, Tb[1] (organism)

RRID:BDSC_38879

Drosophila melanogaster with name P{w[+mC]=alphaTub84B(FRT.GAL80)}1, w[*]; Bl[1]/CyO; TM2/TM6B, Tb[1] from BDSC.

GFP antibody (antibody)

RRID:AB_10710875

This polyclonal targets GFP antibody

pBS-KS-attB2-SA(1)-T2A-QF2-Hsp70 (plasmid)

RRID:Addgene_62945

Myocyte enhancer factor 2 antibody (antibody)

RRID:AB_2568604

This polyclonal targets Mef2

pBS-KS-attB2-SA(0)-T2A-QF2-Hsp70 (plasmid)

RRID:Addgene_62944

P{ry[+t7.2]=neoFRT}42D (organism)

RRID:BDSC_1802

Drosophila melanogaster with name P{ry[+t7.2]=neoFRT}42D from BDSC.