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Publication

An ancient germ cell-specific RNA-binding protein protects the germline from cryptic splice site poisoning.

eLife | 2019

Male germ cells of all placental mammals express an ancient nuclear RNA binding protein of unknown function called RBMXL2. Here we find that deletion of the retrogene encoding RBMXL2 blocks spermatogenesis. Transcriptome analyses of age-matched deletion mice show that RBMXL2 controls splicing patterns during meiosis. In particular, RBMXL2 represses the selection of aberrant splice sites and the insertion of cryptic and premature terminal exons. Our data suggest a Rbmxl2 retrogene has been conserved across mammals as part of a splicing control mechanism that is fundamentally important to germ cell biology. We propose that this mechanism is essential to meiosis because it buffers the high ambient concentrations of splicing activators, thereby preventing poisoning of key transcripts and disruption to gene expression by aberrant splice site selection.

Pubmed ID: 30674417 RIS Download

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Associated grants

Agency: NIA NIH HHS, United States
Id: R01 AG046544
Agency: Medical Research Council, United Kingdom
Id: MC_UU_00007/6
Agency: Medical Research Council, United Kingdom
Id: MC_PC_U127580973
Agency: Wellcome Trust, United Kingdom
Agency: Wellcome Trust, United Kingdom
Id: 089225/Z/09/Z
Agency: Biotechnology and Biological Sciences Research Council, United Kingdom
Id: BB/P006612/1
Agency: NIH HHS, United States
Id: R01 AG AG046544

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