Thermosensation is critical for avoiding thermal extremes and regulating body temperature. While thermosensors activated by noxious temperatures respond to hot or cold, many innocuous thermosensors exhibit robust baseline activity and lack discrete temperature thresholds, suggesting they are not simply warm and cool detectors. Here, we investigate how the aristal Cold Cells encode innocuous temperatures in Drosophila. We find they are not cold sensors but cooling-activated and warming-inhibited phasic thermosensors that operate similarly at warm and cool temperatures; we propose renaming them "Cooling Cells." Unexpectedly, Cooling Cell thermosensing does not require the previously reported Brivido Transient Receptor Potential (TRP) channels. Instead, three Ionotropic Receptors (IRs), IR21a, IR25a, and IR93a, specify both the unique structure of Cooling Cell cilia endings and their thermosensitivity. Behaviorally, Cooling Cells promote both warm and cool avoidance. These findings reveal a morphogenetic role for IRs and demonstrate the central role of phasic thermosensing in innocuous thermosensation. VIDEO ABSTRACT.
Pubmed ID: 30654923 RIS Download
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View all literature mentionsSoftware for image processing, analysis, and editing. The software includes features such as touch capabilities, a customizable toolbar, 2D and 3D image merging, and Cloud access and options.
View all literature mentionsCollects, maintains and distributes Drosophila melanogaster strains for research. Emphasis is placed on genetic tools that are useful to a broad range of investigations. These include basic stocks of flies used in genetic analysis such as marker, balancer, mapping, and transposon-tagging strains; mutant alleles of identified genes, including a large set of transposable element insertion alleles; defined sets of deficiencies and a variety of other chromosomal aberrations; engineered lines for somatic and germline clonal analysis; GAL4 and UAS lines for targeted gene expression; enhancer trap and lacZ-reporter strains with defined expression patterns for marking tissues; and a collection of transposon-induced lethal mutations.
View all literature mentionsThis polyclonal targets Ir25a
View all literature mentionsDrosophila melanogaster with name w[1118]; P{y[+t7.7] w[+mC]=GMR11F02-GAL4}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; TI{w[+mW.hs]=TI}Ir25a[2]/CyO from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=Ir40a-GAL4.3011}214.1; TM2/TM6B, Tb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{y[+t7.7] w[+mC]=20XUAS-IVS-GCaMP6m}attP40 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{y[+mDint2]=MIC}Ir93a[MI05555] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=Ir25a-GAL4.A}236.1; TM2/TM6B, Tb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; Mi{y[+mDint2]=MIC}Ir93a[MI05555] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; TI{w[+mW.hs]=TI}Ir25a[2]/CyO from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{y[+t7.7] w[+mC]=20XUAS-IVS-GCaMP6m}attP40 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=Ir25a-GAL4.A}236.1; TM2/TM6B, Tb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{y[+t7.7] w[+mC]=GMR11F02-GAL4}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=Ir40a-GAL4.3011}214.1; TM2/TM6B, Tb[1] from BDSC.
View all literature mentions