Iron metabolism is an essential process that when dysregulated causes disease. Mammalian serum transferrin (TF) plays a primary role in delivering iron to cells. To improve our understanding of the conservation of iron metabolism between species, we investigate here the function of the TF homolog in Drosophila melanogaster, transferrin 1 (Tsf1). Tsf1 knockdown results in iron accumulation in the gut and iron deficiency in the fat body (which is analogous to the mammalian liver). Fat body-derived Tsf1 localizes to the gut surface, suggesting that Tsf1 functions in trafficking iron between the gut and the fat body, similar to TF in mammals. Moreover, Tsf1 knockdown strongly suppresses the phenotypic effects of ferritin (Fer1HCH) RNAi, an established iron trafficker in Drosophila. We propose that Tsf1 and ferritin compete for iron in the Drosophila intestine and demonstrate the value of using Drosophila for investigating iron trafficking and the evolution of systemic iron regulation.
Pubmed ID: 30650364 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
Statistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsTHIS RESOURCE IS NO LONGER IN SERVICE. Documented on May 18,2023.
View all literature mentionsSoftware application with data analysis tools and spreadsheet templates to track and visualize data. It is used to manage and process data.
View all literature mentionsThis monoclonal targets β-Actin
View all literature mentionsThis unknown targets IgG
View all literature mentionsDrosophila melanogaster with name w[1118]; P{w[+mC]=UAS-GFP.nls}14 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{w[+mC]=Cg-GAL4.A}2 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{GMR-GAL4.w[-]}2/CyO from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; PBac{y[+mDint2] w[+mC]=20XUAS-IVS-GCaMP6m}VK00005 from BDSC.
View all literature mentionsDrosophila melanogaster with name P{w[+mW.hs]=GawB}elav[C155] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118] from BDSC.
View all literature mentionsLight cycler software for analysis of quatitative real time PCR
View all literature mentions