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Many functions of glial cells depend on the formation of selective glial networks mediated by gap junctions formed by members of the connexin family. Olfactory ensheathing cells (OECs) are specialized glia associated with olfactory sensory neuron axons. Like other glia, they form selective networks, however, the connexins that support OEC connectivity in vivo have not been identified. We used an in vivo mouse model to selectively delete candidate connexin genes with temporal control from OECs and address the physiological consequences. Using this model, we effectively abolished the expression of connexin 43 (Cx43) in OECs in both juvenile and adult mice. Cx43-deleted OECs exhibited features consistent with the loss of gap junctions including reduced membrane conductance, largely reduced sensitivity to the gap junction blocker meclofenamic acid and loss of dye coupling. This indicates that Cx43, a typically astrocytic connexin, is the main connexin forming functional channels in OECs. Despite these changes in functional properties, the deletion of Cx43 deletion did not alter the density of OECs. The strategy used here may prove useful to delete other candidate genes to better understand the functional roles of OECs in vivo.
Pubmed ID: 30628061 RIS Download
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This monoclonal targets GFAP
View all literature mentionsThis polyclonal targets Brain lipid binding protein
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis monoclonal targets Gja1
View all literature mentionsThis unknown targets Connexin 43
View all literature mentionsThis unknown targets Connexin 30
View all literature mentionsMus musculus with name B6.129S7-Gja1tm1Dlg/J from IMSR.
View all literature mentionsMus musculus with name B6.Cg-Tg(Plp1-cre/ERT)3Pop/J from IMSR.
View all literature mentionsMus musculus with name B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J from IMSR.
View all literature mentionsMus musculus with name B6.129S7-Gja1tm1Dlg/J from IMSR.
View all literature mentionsThis polyclonal targets Brain lipid binding protein
View all literature mentionsThis polyclonal targets Brain lipid binding protein
View all literature mentionsThis monoclonal targets GFAP
View all literature mentionsThis polyclonal targets Brain lipid binding protein
View all literature mentionsThis unknown targets Connexin 30
View all literature mentionsThis monoclonal targets Gja1
View all literature mentionsThis unknown targets Connexin 43
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsMus musculus with name B6.129S7-Gja1tm1Dlg/J from IMSR.
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis monoclonal targets Gja1
View all literature mentionsMus musculus with name B6.Cg-Tg(Plp1-cre/ERT)3Pop/J from IMSR.
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