Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Publication

SPOP Promotes Nanog Destruction to Suppress Stem Cell Traits and Prostate Cancer Progression.

Developmental cell | 2019

Frequent SPOP mutation defines the molecular feature underlying one of seven sub-types of human prostate cancer (PrCa). However, it remains largely elusive how SPOP functions as a tumor suppressor in PrCa. Here, we report that SPOP suppresses stem cell traits of both embryonic stem cells and PrCa cells through promoting Nanog poly-ubiquitination and subsequent degradation. Mechanistically, Nanog, but not other pluripotency-determining factors including Oct4, Sox2, and Klf4, specifically interacts with SPOP via a conservative degron motif. Importantly, cancer-derived mutations in SPOP or at the Nanog-degron (S68Y) disrupt SPOP-mediated destruction of Nanog, leading to elevated cancer stem cell traits and PrCa progression. Notably, we identify the Pin1 oncoprotein as an upstream Nanog regulator that impairs its recognition by SPOP and thereby stabilizes Nanog. Thus, Pin1 inhibitors promote SPOP-mediated destruction of Nanog, which provides the molecular insight and rationale to use Pin1 inhibitor(s) for targeted therapies of PrCa patients with wild-type SPOP.

Pubmed ID: 30595538 RIS Download

Research resources used in this publication

Additional research tools detected in this publication

None found

Antibodies used in this publication

Associated grants

  • Agency: NCI NIH HHS, United States
    Id: P01 CA203655
  • Agency: NCI NIH HHS, United States
    Id: R01 CA200651
  • Agency: NIGMS NIH HHS, United States
    Id: R01 GM094777
  • Agency: NCI NIH HHS, United States
    Id: R01 CA200573
  • Agency: NCI NIH HHS, United States
    Id: K99 CA212292
  • Agency: NCI NIH HHS, United States
    Id: R01 CA177910
  • Agency: NIGMS NIH HHS, United States
    Id: R01 GM089763
  • Agency: NCI NIH HHS, United States
    Id: R01 CA063113
  • Agency: NCI NIH HHS, United States
    Id: R01 CA229307

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

This is a list of tools and resources that we have found mentioned in this publication.

Mouse Anti-Human CD26 Monoclonal Antibody, Allophycocyanin Conjugated, Clone BA5b (antibody)

RRID:AB_1468826

This monoclonal targets Human CD26

View all literature mentions

Mouse IgG2a kappa Isotype Control (eBM2a), APC, eBioscience (antibody)

RRID:AB_10598641

This isotype control targets Not Applicable

View all literature mentions

Mouse IgG2a kappa Isotype Control (eBM2a), PE, eBioscience (antibody)

RRID:AB_1603328

This isotype control targets Not Applicable

View all literature mentions

Monoclonal ANTI-FLAG® M2 antibody (antibody)

RRID:AB_259529

This monoclonal targets FLAG

View all literature mentions

HA-probe Antibody (Y-11) (antibody)

RRID:AB_631618

This polyclonal targets HA-probe

View all literature mentions

Anti-Mouse IgG (whole molecule)-Peroxidase antibody produced in goat (antibody)

RRID:AB_258167

This polyclonal targets Mouse IgG (whole molecule)-Peroxidase antibody produced in goat

View all literature mentions

Human CD133 Alexa Fluor® 488-conjugated Antibody (antibody)

RRID:AB_2756395

This monoclonal targets CD133

View all literature mentions

p27 (N-20) (antibody)

RRID:AB_632131

This polyclonal targets p27 (N-20)

View all literature mentions

Living Colors® GFP Monoclonal Antibody (antibody)

RRID:AB_2756343

This monoclonal targets GFP

View all literature mentions

ANTI-FLAG(R) antibody produced in rabbit (antibody)

RRID:AB_439687

This polyclonal targets FLAG

View all literature mentions

HA-influenza (antibody)

RRID:AB_257974

This monoclonal targets HA

View all literature mentions

HA.11 Clone 16B12 Monoclonal Antibody, Purified (antibody)

RRID:AB_10064068

This monoclonal targets HA.11 Clone 16B12 Purified

View all literature mentions

SPOP antibody (antibody)

RRID:AB_2756394

This polyclonal targets SPOP

View all literature mentions

Rabbit Anti-Myc-Tag Monoclonal Antibody, Unconjugated, Clone 71D10 (antibody)

RRID:AB_490778

This monoclonal targets Myc-Tag

View all literature mentions

PE anti-human CD117 (c-kit) (antibody)

RRID:AB_314983

This monoclonal targets CD117

View all literature mentions

Myc-Tag (9B11) Mouse mAb (antibody)

RRID:AB_331783

This monoclonal targets c-Myc

View all literature mentions

Mouse IgG2A Alexa Fluor 488 Isotype Control (Clone 20102) (antibody)

RRID:AB_10718683

This unknown targets Mouse IgG2A Alexa Fluor 488 Isotype Control (Clone 20102)

View all literature mentions

CD24 Monoclonal Antibody (eBioSN3 (SN3 A5-2H10)), APC, eBioscience (antibody)

RRID:AB_10718529

This monoclonal targets CD24

View all literature mentions

Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in goat (antibody)

RRID:AB_258207

This unknown targets Rabbit IgG, whole molecule

View all literature mentions

Trop2 (EGP-1) Monoclonal Antibody (MR54), PE, eBioscience (antibody)

RRID:AB_2572648

This monoclonal targets Trop2 (EGP-1)

View all literature mentions

ANTI-FLAG(R) M2 Affinity Gel (antibody)

RRID:AB_10063035

This unknown targets ANTI-FLAG(R) M2 Affinity Gel

View all literature mentions

Monoclonal Anti-alpha-Tubulin antibody produced in mouse (antibody)

RRID:AB_477579

This monoclonal targets α-tubulin

View all literature mentions

cyclin A (H-432) (antibody)

RRID:AB_631329

This polyclonal targets CCNA2

View all literature mentions

CD26 Monoclonal Antibody (2A6), PE, eBioscience™ (antibody)

RRID:AB_2572554

This monoclonal targets CD26

View all literature mentions

Mouse Anti-Chicken Vinculin Monoclonal Antibody, Unconjugated, Clone VIN-11-5 (antibody)

RRID:AB_477617

This monoclonal targets Vinculin

View all literature mentions

CD49f (Integrin alpha 6) Monoclonal Antibody (eBioGoH3 (GoH3)), APC, eBioscience (antibody)

RRID:AB_2016694

This monoclonal targets CD49f (Integrin alpha 6)

View all literature mentions

Nanog (D73G4) XP Rabbit Antibody (antibody)

RRID:AB_10559205

This monoclonal targets Nanog

View all literature mentions

CUL3 Antibody (antibody)

RRID:AB_2086432

This polyclonal targets Cul3

View all literature mentions

GST (91G1) Rabbit mAb (antibody)

RRID:AB_490796

This monoclonal targets GST (91G1) Rabbit mAb

View all literature mentions

Anti-Nanog, N-terminus (antibody)

RRID:AB_2267042

This polyclonal targets Nanog

View all literature mentions

cyclin E (HE12) (antibody)

RRID:AB_627357

This monoclonal targets cyclin E (HE12)

View all literature mentions

Human CD133 Alexa Fluor® 488-conjugated Antibody (antibody)

RRID:AB_2756395

This monoclonal targets CD133

View all literature mentions

SPOP antibody (antibody)

RRID:AB_2756394

This polyclonal targets SPOP

View all literature mentions

Anti-Nanog, N-terminus (antibody)

RRID:AB_2267042

This polyclonal targets Nanog

View all literature mentions

CUL3 Antibody (antibody)

RRID:AB_2086432

This polyclonal targets Cul3

View all literature mentions

Nanog (D73G4) XP Rabbit Antibody (antibody)

RRID:AB_10559205

This monoclonal targets Nanog

View all literature mentions

Rabbit Anti-Myc-Tag Monoclonal Antibody, Unconjugated, Clone 71D10 (antibody)

RRID:AB_490778

This monoclonal targets Myc-Tag

View all literature mentions

HA-probe Antibody (Y-11) (antibody)

RRID:AB_631618

This polyclonal targets HA-probe

View all literature mentions

cyclin A (H-432) (antibody)

RRID:AB_631329

This polyclonal targets CCNA2

View all literature mentions

Myc-Tag (9B11) Mouse mAb (antibody)

RRID:AB_331783

This monoclonal targets c-Myc

View all literature mentions

p27 (N-20) (antibody)

RRID:AB_632131

This polyclonal targets p27 (N-20)

View all literature mentions

cyclin E (HE12) (antibody)

RRID:AB_627357

This monoclonal targets cyclin E (HE12)

View all literature mentions

GST (91G1) Rabbit mAb (antibody)

RRID:AB_490796

This monoclonal targets GST (91G1) Rabbit mAb

View all literature mentions

Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in goat (antibody)

RRID:AB_258207

This unknown targets Rabbit IgG, whole molecule

View all literature mentions

ANTI-FLAG(R) M2 Affinity Gel (antibody)

RRID:AB_10063035

This unknown targets ANTI-FLAG(R) M2 Affinity Gel

View all literature mentions

HA-influenza (antibody)

RRID:AB_257974

This monoclonal targets HA

View all literature mentions

Monoclonal Anti-alpha-Tubulin antibody produced in mouse (antibody)

RRID:AB_477579

This monoclonal targets α-tubulin

View all literature mentions

Monoclonal ANTI-FLAG® M2 antibody (antibody)

RRID:AB_259529

This monoclonal targets FLAG

View all literature mentions

Mouse Anti-Chicken Vinculin Monoclonal Antibody, Unconjugated, Clone VIN-11-5 (antibody)

RRID:AB_477617

This monoclonal targets Vinculin

View all literature mentions

HA.11 Clone 16B12 Monoclonal Antibody, Purified (antibody)

RRID:AB_10064068

This monoclonal targets HA.11 Clone 16B12 Purified

View all literature mentions

Mouse Anti-Human CD26 Monoclonal Antibody, Allophycocyanin Conjugated, Clone BA5b (antibody)

RRID:AB_1468826

This monoclonal targets Human CD26

View all literature mentions

PE anti-human CD117 (c-kit) (antibody)

RRID:AB_314983

This monoclonal targets CD117

View all literature mentions

CD26 Monoclonal Antibody (2A6), PE, eBioscience™ (antibody)

RRID:AB_2572554

This monoclonal targets CD26

View all literature mentions

Mouse IgG2a kappa Isotype Control (eBM2a), PE, eBioscience (antibody)

RRID:AB_1603328

This isotype control targets Not Applicable

View all literature mentions

CD49f (Integrin alpha 6) Monoclonal Antibody (eBioGoH3 (GoH3)), APC, eBioscience (antibody)

RRID:AB_2016694

This monoclonal targets CD49f (Integrin alpha 6)

View all literature mentions

Anti-Mouse IgG (whole molecule)-Peroxidase antibody produced in goat (antibody)

RRID:AB_258167

This polyclonal targets Mouse IgG (whole molecule)-Peroxidase antibody produced in goat

View all literature mentions

Trop2 (EGP-1) Monoclonal Antibody (MR54), PE, eBioscience (antibody)

RRID:AB_2572648

This monoclonal targets Trop2 (EGP-1)

View all literature mentions

Living Colors® GFP Monoclonal Antibody (antibody)

RRID:AB_2756343

This monoclonal targets GFP

View all literature mentions

ANTI-FLAG(R) antibody produced in rabbit (antibody)

RRID:AB_439687

This polyclonal targets FLAG

View all literature mentions

Mouse IgG2a kappa Isotype Control (eBM2a), APC, eBioscience (antibody)

RRID:AB_10598641

This isotype control targets Not Applicable

View all literature mentions

Mouse IgG2A Alexa Fluor 488 Isotype Control (Clone 20102) (antibody)

RRID:AB_10718683

This unknown targets Mouse IgG2A Alexa Fluor 488 Isotype Control (Clone 20102)

View all literature mentions

CD24 Monoclonal Antibody (eBioSN3 (SN3 A5-2H10)), APC, eBioscience (antibody)

RRID:AB_10718529

This monoclonal targets CD24

View all literature mentions

About

The SciCrunch Infrastructure was developed as a cooperative data platform to be used by diverse communities in making data more FAIR.

Recent News Entries

New Release: NeuroMorpho.Org Version 8.6 (12/4/23), with 14,452 new tracings from 45 datasets

Dr. Martone on BrainFacts.org a FAIR video

With 2023 there are new NIH guidelines on data deposition, are you ready?

Contact Us

FAIR Data Informatics Lab

University of California, San Diego
9500 Gilman Drive, Mail Code 0608
La Jolla, CA 92093-0608
United States

info  scicrunch.org

About SciCrunch | Privacy Policy | Terms of Service

SciCrunch