Human primordial germ cells (hPGCs) are the first embryonic progenitors in the germ cell lineage, yet the molecular mechanisms required for hPGC formation are not well characterized. To identify regulatory regions in hPGC development, we used the assay for transposase-accessible chromatin using sequencing (ATAC-seq) to systematically characterize regions of open chromatin in hPGCs and hPGC-like cells (hPGCLCs) differentiated from human embryonic stem cells (hESCs). We discovered regions of open chromatin unique to hPGCs and hPGCLCs that significantly overlap with TFAP2C-bound enhancers identified in the naive ground state of pluripotency. Using CRISPR/Cas9, we show that deleting the TFAP2C-bound naive enhancer at the OCT4 locus (also called POU5F1) results in impaired OCT4 expression and a negative effect on hPGCLC identity.
Pubmed ID: 30590035 RIS Download
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Original SAMTOOLS package has been split into three separate repositories including Samtools, BCFtools and HTSlib. Samtools for manipulating next generation sequencing data used for reading, writing, editing, indexing,viewing nucleotide alignments in SAM,BAM,CRAM format. BCFtools used for reading, writing BCF2,VCF, gVCF files and calling, filtering, summarising SNP and short indel sequence variants. HTSlib used for reading, writing high throughput sequencing data.
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View all literature mentionsThis monoclonal targets CD49f
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View all literature mentionsThis polyclonal targets Human KLF4 Affinity Purified Ab
View all literature mentionsThis polyclonal targets POU5F1
View all literature mentionsThis monoclonal targets Human BLIMP1/PRDM1 MAb (Clone 646702)
View all literature mentionsThis monoclonal targets CD117 (c-Kit)
View all literature mentionsThis monoclonal targets CD326
View all literature mentionsThis monoclonal targets Alkaline Phosphatase
View all literature mentionsThis monoclonal targets CD326
View all literature mentionsThis monoclonal targets CD49f
View all literature mentionsThis monoclonal targets CD49f
View all literature mentionsThis monoclonal targets Human TFAP2C
View all literature mentionsThis unknown targets ND
View all literature mentionsThis monoclonal targets Human AP2 gamma
View all literature mentionsThis monoclonal targets Histone H3 antibody [mAbcam 10799] - ChIP Grade
View all literature mentions