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Publication

The TFAP2C-Regulated OCT4 Naive Enhancer Is Involved in Human Germline Formation.

Cell reports | 2018

Human primordial germ cells (hPGCs) are the first embryonic progenitors in the germ cell lineage, yet the molecular mechanisms required for hPGC formation are not well characterized. To identify regulatory regions in hPGC development, we used the assay for transposase-accessible chromatin using sequencing (ATAC-seq) to systematically characterize regions of open chromatin in hPGCs and hPGC-like cells (hPGCLCs) differentiated from human embryonic stem cells (hESCs). We discovered regions of open chromatin unique to hPGCs and hPGCLCs that significantly overlap with TFAP2C-bound enhancers identified in the naive ground state of pluripotency. Using CRISPR/Cas9, we show that deleting the TFAP2C-bound naive enhancer at the OCT4 locus (also called POU5F1) results in impaired OCT4 expression and a negative effect on hPGCLC identity.

Pubmed ID: 30590035 RIS Download

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Associated grants

Agency: NICHD NIH HHS, United States
Id: R01 HD079546
Agency: NICHD NIH HHS, United States
Id: R24 HD000836

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This is a list of tools and resources that we have found mentioned in this publication.

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