Enlargement of the lymphatic vascular network in tumor-draining lymph nodes (LNs) often precedes LN metastasis, likely providing a lymphovascular niche for tumor cells. We investigated morphological and molecular changes associated with the lymphatic remodeling process, using the 4T1 breast cancer and B16F10 melanoma models. Lymphatic expansion in tumor-draining LNs is mediated by sprouting and proliferation of lymphatic endothelial cells (LECs) as early as 4 days after tumor implantation. RNA sequencing revealed an altered transcriptional profile of LECs from tumor-draining compared to naive LNs with similar changes in both tumor models. Integrin αIIb is upregulated in LECs of tumor-draining LNs and mediates LEC adhesion to fibrinogen in vitro. LEC-associated fibrinogen was also detected in LNs in vivo, suggesting a role of integrin αIIb in lymphatic remodeling. Together, our results identify specific responses of LN LECs to tumor stimuli and provide insights into the mechanisms of lymphovascular niche formation in tumor-draining LNs.
Pubmed ID: 30590031 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
Global nonprofit biological resource center (BRC) and research organization that provides biological products, technical services and educational programs to private industry, government and academic organizations. Its mission is to acquire, authenticate, preserve, develop and distribute biological materials, information, technology, intellectual property and standards for the advancement and application of scientific knowledge. The primary purpose of ATCC is to use its resources and experience as a BRC to become the world leader in standard biological reference materials management, intellectual property resource management and translational research as applied to biomaterial development, standardization and certification. ATCC characterizes cell lines, bacteria, viruses, fungi and protozoa, as well as develops and evaluates assays and techniques for validating research resources and preserving and distributing biological materials to the public and private sector research communities.
View all literature mentionsSoftware package as distribution of ImageJ and ImageJ2 together with Java, Java3D and plugins organized into coherent menu structure. Used to assist research in life sciences.
View all literature mentionsStatistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsSoftware package for interpreting gene expression data. Used for interpretation of a large-scale experiment by identifying pathways and processes.
View all literature mentionsCommercial supplier and developer of in vivo antibodies. Provides antibodies and antibody production services.
View all literature mentionsImaris provides range of capabilities for working with three dimensional images. Uses flexible editing and processing functions, such as interactive surface rendering and object slicing capabilities. And output to standard TIFF, Quicktime and AVI formats. Imaris accepts virtually all image formats that are used in confocal microscopy and many of those used in wide-field image acquisition.
View all literature mentionsA commercial organization which provides assay technologies to isolate DNA, RNA, and proteins from any biological sample. Assay technologies are then used to make specific target biomolecules, such as the DNA of a specific virus, visible for subsequent analysis.
View all literature mentionsSoftware package for differential gene expression analysis based on the negative binomial distribution. Used for analyzing RNA-seq data for differential analysis of count data, using shrinkage estimation for dispersions and fold changes to improve stability and interpretability of estimates.
View all literature mentionslaboratory mouse with name BALB/cAnNCrl from MGI.
View all literature mentionslaboratory mouse with name C57BL/6JRj from MGI.
View all literature mentionsMus musculus with name C57BL/6J from IMSR.
View all literature mentionsThis polyclonal targets ITGA2B antibody produced in rabbit
View all literature mentionsThis polyclonal targets Tyrosine Hydroxylase
View all literature mentionsThis polyclonal targets Mouse Osteoactivin / GPNMB
View all literature mentionsThis monoclonal targets CD41
View all literature mentionsThis monoclonal targets Podoplanin
View all literature mentionsThis monoclonal targets Ki-67
View all literature mentionsThis polyclonal targets Mouse LYVE-1
View all literature mentionsThis monoclonal targets CD45
View all literature mentionsThis polyclonal targets Human LYVE-1 ylated Affinity Purified PAb
View all literature mentionsThis monoclonal targets CD45.2
View all literature mentionsThis recombinant monoclonal targets Melanoma gp100
View all literature mentionsThis monoclonal targets CD31
View all literature mentionsThis monoclonal targets Mki67
View all literature mentionsThis polyclonal targets Human Fibrinogen
View all literature mentionsThis polyclonal targets Human Prox1
View all literature mentionsThis monoclonal targets CD41
View all literature mentionsThis isotype control targets KLH
View all literature mentionsThis polyclonal targets Cytokeratin
View all literature mentionsThis monoclonal targets IFNγ
View all literature mentionsThis unknown targets IgG from rat serum
View all literature mentionsCell line B16-F10 is a Cancer cell line with a species of origin Mus musculus (Mouse)
View all literature mentionsCell line 4T1-luc2 [Caliper] is a Cancer cell line with a species of origin Mus musculus (Mouse)
View all literature mentionsThis polyclonal targets ITGA2B antibody produced in rabbit
View all literature mentionsCell line B16-F10 is a Cancer cell line with a species of origin Mus musculus (Mouse)
View all literature mentionsThis monoclonal targets CD45
View all literature mentionsThis monoclonal targets CD31
View all literature mentionsThis polyclonal targets Human Prox1
View all literature mentionsThis isotype control targets KLH
View all literature mentionsThis monoclonal targets Mki67
View all literature mentionsThis monoclonal targets IFNγ
View all literature mentionsThis unknown targets IgG from rat serum
View all literature mentionsThis monoclonal targets Ki-67
View all literature mentionsThis monoclonal targets Podoplanin
View all literature mentionsCell line 4T1-luc2 [Caliper] is a Cancer cell line with a species of origin Mus musculus (Mouse)
View all literature mentionsThis monoclonal targets CD45.2
View all literature mentionsThis polyclonal targets Tyrosine Hydroxylase
View all literature mentionsThis polyclonal targets Mouse LYVE-1
View all literature mentionsThis polyclonal targets Mouse Osteoactivin / GPNMB
View all literature mentionsThis monoclonal targets CD41
View all literature mentionsThis polyclonal targets Cytokeratin
View all literature mentionsThis polyclonal targets Human LYVE-1 ylated Affinity Purified PAb
View all literature mentionsThis recombinant monoclonal targets Melanoma gp100
View all literature mentionsThis polyclonal targets Human Fibrinogen
View all literature mentionsThis monoclonal targets CD41
View all literature mentions