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Oligodendrocytes (OLs) are myelinating cells of the central nervous system. Recent studies have shown that mechanical factors influence various cell properties. Mechanical stimulation can be transduced into intracellular biochemical signals through mechanosensors, such as integrin, p130Cas, talin and vinculin. However, the molecular mechanisms underlying the mechanical regulation of OLs by mechanosensors remain largely unknown. We found that morphology of OL was affected by knockdown of the mechanosensors p130Cas or talin1. Stretching of OL precursor cells induced the phosphorylation of p130Cas and talin-associated assembly of vinculin. Shear stress decreased the number of OL processes, whereas these effects were mechanically suppressed by dominant-negative (DN) p130Cas, but not by DN-talin1. To investigate the roles of p130Cas in post-natal OLs in vivo, we constructed a novel p130Cas knock-in mouse and found overexpression of p130Cas in vivo affected the number of mature OLs in the cortex. These results indicate that the mechanosensor p130Cas controls both OL morphogenesis and maturation.
Pubmed ID: 30589943 RIS Download
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This monoclonal targets Vinculin antibody produced in mouse
View all literature mentionsThis polyclonal targets Phospho-p130 Cas (Tyr165)
View all literature mentionsThis monoclonal targets ND
View all literature mentionsThis polyclonal targets Talin 1 antibody
View all literature mentionsCell line Phoenix-Eco is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsMus musculus with name B6(129)-Tg(Plp1-tTA)1Ike/KftnkRbrc from IMSR.
View all literature mentionsMus musculus with name C57BL/6J from IMSR.
View all literature mentionsThis polyclonal targets Talin 1 antibody
View all literature mentionsMus musculus with name C57BL/6J from IMSR.
View all literature mentionsCell line Phoenix-Eco is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsMus musculus with name B6(129)-Tg(Plp1-tTA)1Ike/KftnkRbrc from IMSR.
View all literature mentionsThis monoclonal targets Vinculin antibody produced in mouse
View all literature mentionsThis monoclonal targets ND
View all literature mentionsThis polyclonal targets Phospho-p130 Cas (Tyr165)
View all literature mentionsThe SciCrunch Infrastructure was developed as a cooperative data platform to be used by diverse communities in making data more FAIR.
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