The discovery of microRNA (miRNA) sorting into extracellular vesicles (EVs) revealed a novel mode of intercellular communication and uncovered a link between cellular endomembrane compartments and small RNAs in EV-secreting cells. Using a two-step ultracentrifugation procedure to isolate EVs released by T cells, we found that 45% of tRNA fragments (tRFs), but fewer than 1% of miRNAs, were significantly enriched in EVs compared with the corresponding cellular RNA. T cell activation induced the EV-mediated release of a specific set of tRFs derived from the 5' end and 3'-internal region of tRNAs without variable loops. Inhibition of EV biogenesis pathways specifically led to the accumulation of these activation-induced EV-enriched tRFs within multivesicular bodies (MVBs). Introducing antisense oligonucleotides to inhibit these tRFs enhanced T cell activation. Taken together, these results demonstrate that T cells selectively release tRFs into EVs via MVBs and suggest that this process may remove tRFs that repress immune activation.
Pubmed ID: 30566862 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
A commercial organization which provides assay technologies to isolate DNA, RNA, and proteins from any biological sample. Assay technologies are then used to make specific target biomolecules, such as the DNA of a specific virus, visible for subsequent analysis.
View all literature mentionsSoftware tool as collection of command line tools for Short-Reads FASTA/FASTQ files preprocessing.
View all literature mentionsA powerful toolset for genome arithmetic allowing one to address common genomics tasks such as finding feature overlaps and computing coverage. Bedtools allows one to intersect, merge, count, complement, and shuffle genomic intervals from multiple files in widely-used genomic file formats such as BAM, BED, GFF/GTF, VCF. While each individual tool is designed to do a relatively simple task (e.g., intersect two interval files), quite sophisticated analyses can be conducted by combining multiple bedtools operations on the UNIX command line.
View all literature mentionsThis monoclonal targets Argonaute 2
View all literature mentionsThis monoclonal targets IL-2
View all literature mentionsThis monoclonal targets CD44
View all literature mentionsThis monoclonal targets CD62L
View all literature mentionsThis unknown targets Goat Mouse IgG (H L)-HRP Conjugate
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis monoclonal targets TIA1, TIAL1
View all literature mentionsThis monoclonal targets Rab7
View all literature mentionsThis polyclonal targets LAMP1
View all literature mentionsThis monoclonal targets beta-Actin
View all literature mentionsThis polyclonal targets Transferrin Receptor antibody
View all literature mentionsThis monoclonal targets CD81
View all literature mentionsThis monoclonal targets tsg 101 (C-2)
View all literature mentionsThis monoclonal targets EIF2C2
View all literature mentionsMus musculus with name C57BL/6J from IMSR.
View all literature mentionsThis polyclonal targets LAMP1
View all literature mentionsThis monoclonal targets CD44
View all literature mentionsThis monoclonal targets IL-2
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis monoclonal targets Rab7
View all literature mentionsThis monoclonal targets TIA1, TIAL1
View all literature mentionsThis unknown targets Goat Mouse IgG (H L)-HRP Conjugate
View all literature mentionsThis monoclonal targets CD81
View all literature mentionsThis polyclonal targets Transferrin Receptor antibody
View all literature mentionsThis monoclonal targets tsg 101 (C-2)
View all literature mentionsThis monoclonal targets beta-Actin
View all literature mentions