Searching across hundreds of databases
The plasticity of developing tissues relies on the adjustment of cell survival and growth rate to environmental cues. This includes the effect of mechanical cues on cell survival. Accordingly, compaction of an epithelium can lead to cell extrusion and cell death. This process was proposed to contribute to tissue homeostasis but also to facilitate the expansion of pretumoral cells through the compaction and elimination of the neighboring healthy cells. However, we know very little about the pathways that can trigger apoptosis upon tissue deformation, and the contribution of compaction-driven death to clone expansion has never been assessed in vivo. Using the Drosophila pupal notum and a new live sensor of ERK, we show first that tissue compaction induces cell elimination through the downregulation of epidermal growth factor receptor/extracellular signal regulated kinase (EGFR/ERK) pathway and the upregulation of the pro-apoptotic protein Hid. Those results suggest that the sensitivity of EGFR/ERK pathway to mechanics could play a more general role in the fine tuning of cell elimination during morphogenesis and tissue homeostasis. Second, we assessed in vivo the contribution of compaction-driven death to pretumoral cell expansion. We found that the activation of the oncogene Ras in clones can downregulate ERK and activate apoptosis in the neighboring cells through their compaction, which eventually contributes to Ras clone expansion. The mechanical modulation of EGFR/ERK during growth-mediated competition for space may contribute to tumor progression.
Pubmed ID: 30554899 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
Multi paradigm numerical computing environment and fourth generation programming language developed by MathWorks. Allows matrix manipulations, plotting of functions and data, implementation of algorithms, creation of user interfaces, and interfacing with programs written in other languages, including C, C++, Java, Fortran and Python. Used to explore and visualize ideas and collaborate across disciplines including signal and image processing, communications, control systems, and computational finance.
View all literature mentionsNon-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
View all literature mentionsSoftware package as distribution of ImageJ and ImageJ2 together with Java, Java3D and plugins organized into coherent menu structure. Used to assist research in life sciences.
View all literature mentionsStatistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsA commercial antibody supplier which supplies primary and secondary antibodies, biochemicals, proteins, peptides, lysates, immunoassays and other kits.
View all literature mentionsAn antibody supplier which banks and distributes hybridomas and monoclonal antibodies for use in research. The bank includes antibodies against targets such as GFP, transcription factors, stem cells, and human.
View all literature mentionsServes Drosophila research community by collecting and distributing DNA clones and vectors; collecting and distributing Drosophila cell lines; developing and testing genomics technologies for use in Drosophila and assisting members of the research community in their use.
View all literature mentionsCell line S2R+ is a Spontaneously immortalized cell line with a species of origin Drosophila melanogaster (Fruit fly)
View all literature mentionsThis monoclonal targets argos
View all literature mentionsThis unknown targets Cleaved Dcp-1 (Asp216)
View all literature mentionsThis polyclonal targets GFP
View all literature mentionsThis monoclonal targets Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204)
View all literature mentionsThis polyclonal targets mCherry
View all literature mentionsThis monoclonal targets dEGF Receptor, Extracellular Domain
View all literature mentionsThis monoclonal targets p44/42 MAPK (Erk1/2)
View all literature mentionsThis monoclonal targets Drosophila ecdysone receptor (EcR common)
View all literature mentionsThis monoclonal targets cadherin, DE- (extracellular domain)
View all literature mentionsThis monoclonal targets tubulin (alpha-)
View all literature mentionsDrosophila melanogaster with name P{w[+mC]=UAS-Pi3K92E.CAAX}1, y[1] w[1118] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.JF01084}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; M{w[+mC]=UAS-hE-Egfr-GFP}ZH-58A/CyO from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; PBac{y[+mDint2] w[+mC]=cic-GFP.FPTB}VK00037 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=UAS-lacZ.Exel}2 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=UAS-sSpiCS}T28 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{y[+t7.7] w[+mC]=UAS-yki.S111A.S168A.S250A.V5}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=UAS-DIAP1.H}3 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=UAS-Raf.gof}F179 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; TI{TI}shg[GFP] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[1118]; P{w[+mW.hs]=GawB}pnr[MD237]/TM3, P{w[+mC]=UAS-y.C}MC2, Ser[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; TI{TI}shg[GFP] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118] from BDSC.
View all literature mentionsThis monoclonal targets tubulin (alpha-)
View all literature mentionsThis monoclonal targets p44/42 MAPK (Erk1/2)
View all literature mentionsThis polyclonal targets GFP
View all literature mentionsThis unknown targets Cleaved Dcp-1 (Asp216)
View all literature mentionsThis monoclonal targets Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204)
View all literature mentionsThis polyclonal targets mCherry
View all literature mentionsThis monoclonal targets argos
View all literature mentionsThis monoclonal targets cadherin, DE- (extracellular domain)
View all literature mentionsThis monoclonal targets Drosophila ecdysone receptor (EcR common)
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=UAS-lacZ.Exel}2 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.JF01084}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name P{w[+mC]=UAS-Pi3K92E.CAAX}1, y[1] w[1118] from BDSC.
View all literature mentionsThis monoclonal targets Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204)
View all literature mentionsThis polyclonal targets GFP
View all literature mentionsThis polyclonal targets mCherry
View all literature mentionsThis monoclonal targets cadherin, DE- (extracellular domain)
View all literature mentionsThis monoclonal targets Drosophila ecdysone receptor (EcR common)
View all literature mentionsThis unknown targets Cleaved Dcp-1 (Asp216)
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; TI{TI}shg[GFP] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; M{w[+mC]=UAS-hE-Egfr-GFP}ZH-58A/CyO from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; PBac{y[+mDint2] w[+mC]=cic-GFP.FPTB}VK00037 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=UAS-DIAP1.H}3 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=UAS-sSpiCS}T28 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{y[+t7.7] w[+mC]=UAS-yki.S111A.S168A.S250A.V5}attP2 from BDSC.
View all literature mentionsThis monoclonal targets p44/42 MAPK (Erk1/2)
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=UAS-Raf.gof}F179 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=UAS-Raf.gof}F179 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=UAS-DIAP1.H}3 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=UAS-sSpiCS}T28 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{y[+t7.7] w[+mC]=UAS-yki.S111A.S168A.S250A.V5}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; TI{TI}shg[GFP] from BDSC.
View all literature mentionsDrosophila melanogaster with name P{w[+mC]=UAS-Pi3K92E.CAAX}1, y[1] w[1118] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; M{w[+mC]=UAS-hE-Egfr-GFP}ZH-58A/CyO from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.JF01084}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; PBac{y[+mDint2] w[+mC]=cic-GFP.FPTB}VK00037 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=UAS-lacZ.Exel}2 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[1118]; P{w[+mW.hs]=GawB}pnr[MD237]/TM3, P{w[+mC]=UAS-y.C}MC2, Ser[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[1118]; P{w[+mW.hs]=GawB}pnr[MD237]/TM3, P{w[+mC]=UAS-y.C}MC2, Ser[1] from BDSC.
View all literature mentionsThis monoclonal targets tubulin (alpha-)
View all literature mentionsThis monoclonal targets dEGF Receptor, Extracellular Domain
View all literature mentionsThis monoclonal targets argos
View all literature mentionsThe SciCrunch Infrastructure was developed as a cooperative data platform to be used by diverse communities in making data more FAIR.
scicrunch.org
