Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Protein Interaction Mapping Identifies RBBP6 as a Negative Regulator of Ebola Virus Replication.

Cell | 2018

Ebola virus (EBOV) infection often results in fatal illness in humans, yet little is known about how EBOV usurps host pathways during infection. To address this, we used affinity tag-purification mass spectrometry (AP-MS) to generate an EBOV-host protein-protein interaction (PPI) map. We uncovered 194 high-confidence EBOV-human PPIs, including one between the viral transcription regulator VP30 and the host ubiquitin ligase RBBP6. Domain mapping identified a 23 amino acid region within RBBP6 that binds to VP30. A crystal structure of the VP30-RBBP6 peptide complex revealed that RBBP6 mimics the viral nucleoprotein (NP) binding to the same interface of VP30. Knockdown of endogenous RBBP6 stimulated viral transcription and increased EBOV replication, whereas overexpression of either RBBP6 or the peptide strongly inhibited both. These results demonstrate the therapeutic potential of biologics that target this interface and identify additional PPIs that may be leveraged for novel therapeutic strategies.

Pubmed ID: 30550789 RIS Download

Associated grants

  • Agency: NIGMS NIH HHS, United States
    Id: P50 GM082250
  • Agency: NIAID NIH HHS, United States
    Id: R01 AI114814
  • Agency: NIAID NIH HHS, United States
    Id: T32 AI060537
  • Agency: NIAID NIH HHS, United States
    Id: U19 AI135990
  • Agency: NIAID NIH HHS, United States
    Id: U19 AI106754
  • Agency: NIAID NIH HHS, United States
    Id: R01 AI120694
  • Agency: NIAID NIH HHS, United States
    Id: P01 AI063302
  • Agency: NIAID NIH HHS, United States
    Id: U19 AI109664
  • Agency: NIAID NIH HHS, United States
    Id: U19 AI109945
  • Agency: Howard Hughes Medical Institute, United States

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

This is a list of tools and resources that we have found mentioned in this publication.


KEGG (tool)

RRID:SCR_012773

An integrated database resource consisting of 16 main databases, broadly categorized into systems information, genomic information, and chemical information. In particular, gene catalogs in the completely sequenced genomes are linked to higher-level systemic functions of the cell, the organism, and the ecosystem. Analysis tools are also available. KEGG may be used as a reference knowledge base for biological interpretation of large-scale datasets generated by sequencing and other high-throughput experimental technologies.

View all literature mentions

ATCC (tool)

RRID:SCR_001672

Global nonprofit biological resource center (BRC) and research organization that provides biological products, technical services and educational programs to private industry, government and academic organizations. Its mission is to acquire, authenticate, preserve, develop and distribute biological materials, information, technology, intellectual property and standards for the advancement and application of scientific knowledge. The primary purpose of ATCC is to use its resources and experience as a BRC to become the world leader in standard biological reference materials management, intellectual property resource management and translational research as applied to biomaterial development, standardization and certification. ATCC characterizes cell lines, bacteria, viruses, fungi and protozoa, as well as develops and evaluates assays and techniques for validating research resources and preserving and distributing biological materials to the public and private sector research communities.

View all literature mentions

Origin (tool)

RRID:SCR_014212

Software application for data analysis and graphing. Origin contains a variety of different graph types, including statistical plots, 2D and 3D vector graphs, and counter graphs. More advance version is OriginPro which offers advanced analysis tools and Apps for Peak Fitting, Surface Fitting, Statistics and Signal Processing.

View all literature mentions

Protein Prospector (tool)

RRID:SCR_014558

A package of over twenty mass spectrometry-based tools primarily geared toward proteomic data analysis and database mining. It can be run from the command line, but is primarily used through a web browser, and there is a public website that allows anyone to use the software without local installation. Tandem mass spectrometry analysis tools are used for database searching and identification of peptides, including post-translationally modified peptides and cross-linked peptides. Support for isotope and label-free quantification from this type of data is provided. MS-Viewer software allows sharing and displaying of annotated spectra from many different tandem mass spectrometry data analysis packages. Other tools include software for analyzing peptide mass fingerprinting data (MS-Fit); prediction of theoretical fragmentation of peptides (MS-Product); theoretical chemical or enzymatic digestion of proteins (MS-Digest); and theoretical modeling of the isotope distribution of any chemical, including peptides (MS-Isotope). Searches using amino acid sequence can be used to identify homologous peptides in a database (MS-Pattern); the use of the combination of amino acid sequence and masses can be used for homologous peptide and protein identification using MS-Homology. Tandem mass spectrometry peak list files can be filtered for the presence of certain peaks or neutral losses using MS-Filter. Given a list of proteins, MS-Bridge can report all potential cross-linked peptide combinations of a specified mass. Given a precursor peptide mass and information about known amino acid presence, absence, or modifications, MS-Comp can report all amino acid combinations that could lead to the observed mass.

View all literature mentions

CellProfiler Image Analysis Software (tool)

RRID:SCR_007358

CellProfiler cell image analysis software is free and open-source software designed to enable biologists without training in computer vision or programming to quantitatively measure phenotypes from thousands of images automatically. It counts cells and also measures the size, shape, intensity and texture of every cell (and every labeled subcellular compartment) in every image. It was designed for high throughput screening but can perform automated image analysis for images from time-lapse movies and low-throughput experiments. CellProfiler has an increasing number of algorithms to identify and measure properties of neuronal cell types.

View all literature mentions

G:Profiler (tool)

RRID:SCR_006809

A web-based toolset for functional profiling of gene lists from large-scale experiments. It has a simple web interface with powerful visualization. It currently supports 85 species, including mammals, fungi, plants, insects, etc, from the Ensembl and Ensembl Genomes databases. g:Profiler consists of the following tools: * g:GOSt retrieves most significant Gene Ontology (GO) terms, KEGG and REACTOME pathways, and TRANSFAC motifs to a user-specified group of genes, proteins or microarray probes. g:GOSt also allows analysis of ranked or ordered lists of genes, visual browsing of GO graph structure, interactive visualization of retrieved results, and many other features. Multiple testing corrections are applied to extract only statistically important results. * g:Convert allows conversion between gene or protein names, database IDs and microarray probes of more than 100 types. A mix of various IDs may be presented as input; output options include HTML, text and XLS spreadsheet. * g:Orth retrieves orthologs for a given set of genes, proteins or probes in a selected organism. Graphical representation also shows orthologs present in all g:Profiler organisms. * g:Sorter searches for similar expression profiles to a given gene, protein or probe in a large set of public microarray datasets from the Gene Expression Omnibus (GEO) database. * g:Cocoa provides a compact interface for comparing enrichments of multiple gene lists. Platform: Online tool

View all literature mentions

CCP4 (tool)

RRID:SCR_007255

Portal for Macromolecular X-Ray Crystallography to produce and support an integrated suite of programs that allows researchers to determine macromolecular structures by X-ray crystallography, and other biophysical techniques. Used in the education and training of scientists in experimental structural biology for determination and analysis of protein structure.

View all literature mentions

QIAGEN (tool)

RRID:SCR_008539

A commercial organization which provides assay technologies to isolate DNA, RNA, and proteins from any biological sample. Assay technologies are then used to make specific target biomolecules, such as the DNA of a specific virus, visible for subsequent analysis.

View all literature mentions

Anti-HA antibody produced in rabbit (antibody)

RRID:AB_260070

This polyclonal antibody targets HA antibody produced in rabbit

View all literature mentions

karyopherin α5 Antibody (1D2) (antibody)

RRID:AB_2721174

This monoclonal antibody targets karyopherin α5

View all literature mentions

Monoclonal ANTI-FLAG® M2 antibody (antibody)

RRID:AB_262044

This monoclonal antibody targets FLAG

View all literature mentions

ANP32A (A-182) antibody (antibody)

RRID:AB_1124655

This monoclonal antibody targets Human ANP32A

View all literature mentions

Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 546 (antibody)

RRID:AB_2534089

This polyclonal antibody targets Mouse IgG (H+L) Highly Cross-Adsorbed

View all literature mentions

Myosin Va (G-4) antibody (antibody)

RRID:AB_10917581

This monoclonal antibody targets Myosin Va (G-4)

View all literature mentions

Flag antibody (antibody)

RRID:AB_439687

This polyclonal antibody targets 8 amino acid sequence (C-asp-tyr-lys-asp-asp-asp-asp-lys-N)

View all literature mentions

RBBP6 Antibody (antibody)

RRID:AB_2721115

This polyclonal antibody targets RBBP6

View all literature mentions