Searching across hundreds of databases
Symbionts play an indispensable role in gut homeostasis, but underlying mechanisms remain elusive. To clarify the role of lactic-acid-producing bacteria (LAB) on intestinal stem-cell (ISC)-mediated epithelial development, we fed mice with LAB-type symbionts such as Bifidobacterium and Lactobacillus spp. Here we show that administration of LAB-type symbionts significantly increased expansion of ISCs, Paneth cells, and goblet cells. Lactate stimulated ISC proliferation through Wnt/β-catenin signals of Paneth cells and intestinal stromal cells. Moreover, Lactobacillus plantarum strains lacking lactate dehydrogenase activity, which are deficient in lactate production, elicited less ISC proliferation. Pre-treatment with LAB-type symbionts or lactate protected mice in response to gut injury provoked by combined treatments with radiation and a chemotherapy drug. Impaired ISC-mediated epithelial development was found in mice deficient of the lactate G-protein-coupled receptor, Gpr81. Our results demonstrate that LAB-type symbiont-derived lactate plays a pivotal role in promoting ISC-mediated epithelial development in a Gpr81-dependent manner.
Pubmed ID: 30543778 RIS Download
Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.
Software for single-cell flow cytometry analysis. Its functions include management, display, manipulation, analysis and publication of the data stream produced by flow and mass cytometers.
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis monoclonal targets TUBB2A
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis monoclonal targets Lgr5/GPR49
View all literature mentionsThis polyclonal targets FKSG80 / GPR81
View all literature mentionsThis monoclonal targets Ki-67
View all literature mentionsThis polyclonal targets IgG H&L
View all literature mentionsThis polyclonal targets PORCN antibody
View all literature mentionsThis monoclonal targets Catenin, beta
View all literature mentionsThis monoclonal targets alpha smooth muscle Actin
View all literature mentionsThis unknown targets Rat IgG (H+L)
View all literature mentionsThis polyclonal targets Human Wnt3
View all literature mentionsThis monoclonal targets GSK-3-alpha / beta, phospho (Ser21 / Ser9)
View all literature mentionsThis unknown targets IgG
View all literature mentionsThis unknown targets Mucin 2
View all literature mentionsThis polyclonal targets OLFM4 antibody
View all literature mentionsThis monoclonal targets CD24
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis monoclonal targets Beta Arrestin 2 antibody
View all literature mentionsThis monoclonal targets CD45
View all literature mentionsThis polyclonal targets LYZ / Lysozyme
View all literature mentionsThis monoclonal targets CD24
View all literature mentionsThis monoclonal targets Human Lysozyme
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal targets Human GPCR GPR49
View all literature mentionsThis polyclonal targets Wnt3a
View all literature mentionsThis monoclonal targets GSK-3-alpha / beta, phospho (Ser21 / Ser9)
View all literature mentionsThis monoclonal targets CD24
View all literature mentionsThis monoclonal targets Human Lysozyme
View all literature mentionsThis polyclonal targets OLFM4 antibody
View all literature mentionsThis monoclonal targets Beta Arrestin 2 antibody
View all literature mentionsThis polyclonal targets FKSG80 / GPR81
View all literature mentionsThis polyclonal targets PORCN antibody
View all literature mentionsThis polyclonal targets IgG H&L
View all literature mentionsThis monoclonal targets alpha smooth muscle Actin
View all literature mentionsThis polyclonal targets LYZ / Lysozyme
View all literature mentionsThis monoclonal targets Lgr5/GPR49
View all literature mentionsThis polyclonal targets Wnt3a
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis unknown targets IgG
View all literature mentionsThis monoclonal targets Catenin, beta
View all literature mentionsThis monoclonal targets CD45
View all literature mentionsThis monoclonal targets Ki-67
View all literature mentionsThis unknown targets Mucin 2
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis monoclonal targets TUBB2A
View all literature mentionsThis unknown targets Rat IgG (H+L)
View all literature mentionsThis monoclonal targets CD24
View all literature mentionsThis polyclonal targets Human GPCR GPR49
View all literature mentionsThis polyclonal targets Human Wnt3
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThe SciCrunch Infrastructure was developed as a cooperative data platform to be used by diverse communities in making data more FAIR.
scicrunch.org
