Complete activation of B cells relies on their capacity to extract tethered antigens from immune synapses by either exerting mechanical forces or promoting their proteolytic degradation through lysosome secretion. Whether antigen extraction can also be tuned by local cues originating from the lymphoid microenvironment has not been investigated. We here show that the expression of Galectin-8-a glycan-binding protein found in the extracellular milieu, which regulates interactions between cells and matrix proteins-is increased within lymph nodes under inflammatory conditions where it enhances B cell arrest phases upon antigen recognition in vivo and promotes synapse formation during BCR recognition of immobilized antigens. Galectin-8 triggers a faster recruitment and secretion of lysosomes toward the B cell-antigen contact site, resulting in efficient extraction of immobilized antigens through a proteolytic mechanism. Thus, extracellular cues can determine how B cells sense and extract tethered antigens and thereby tune B cell responses in vivo.
Pubmed ID: 30540943 RIS Download
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Software package as distribution of ImageJ and ImageJ2 together with Java, Java3D and plugins organized into coherent menu structure. Used to assist research in life sciences.
View all literature mentionsOpen source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.
View all literature mentionsImaris provides range of capabilities for working with three dimensional images. Uses flexible editing and processing functions, such as interactive surface rendering and object slicing capabilities. And output to standard TIFF, Quicktime and AVI formats. Imaris accepts virtually all image formats that are used in confocal microscopy and many of those used in wide-field image acquisition.
View all literature mentionsSoftware for single-cell flow cytometry analysis. Its functions include management, display, manipulation, analysis and publication of the data stream produced by flow and mass cytometers.
View all literature mentionsCenter that produces knockout mice and carries out high-throughput phenotyping of each line in order to determine function of every gene in mouse genome. These mice will be preserved in repositories and made available to scientific community representing valuable resource for basic scientific research as well as generating new models for human diseases.
View all literature mentionsGlobal nonprofit biological resource center (BRC) and research organization that provides biological products, technical services and educational programs to private industry, government and academic organizations. Its mission is to acquire, authenticate, preserve, develop and distribute biological materials, information, technology, intellectual property and standards for the advancement and application of scientific knowledge. The primary purpose of ATCC is to use its resources and experience as a BRC to become the world leader in standard biological reference materials management, intellectual property resource management and translational research as applied to biomaterial development, standardization and certification. ATCC characterizes cell lines, bacteria, viruses, fungi and protozoa, as well as develops and evaluates assays and techniques for validating research resources and preserving and distributing biological materials to the public and private sector research communities.
View all literature mentionsRandomized controlled trial being conducted at two clinical centers in the United States to learn more about the effects of weight loss on urinary incontinence. About 330 overweight women aged 30 or older will participate and will be followed for 18 months. Efficacy of weight reduction as a treatment for urinary incontinence will be examined at 6 months following the intensive weight control program, and the sustained impact of the intervention will be examined at 18 months. To increase the maintenance of weight reduction and facilitate evaluation of the enduring impact of weight loss on urinary incontinence, they propose to study a motivation-based weight maintenance program. At the end of the intensive weight control program, women randomized to the weight loss program will be randomized to either a 12-month skill-based maintenance intervention or to a motivation-based maintenance intervention. The maintenance interventions maximize the potential for sustained weight loss and will allow them to determine if long-term weight reduction will produce continued improvement in urinary incontinence.
View all literature mentionsA commercial antibody supplier which supplies primary and secondary antibodies, biochemicals, proteins, peptides, lysates, immunoassays and other kits.
View all literature mentionsMus musculus with name B6.SJL-Ptprca Pepc/BoyJ from IMSR.
View all literature mentionsMus musculus with name C57BL/6J from IMSR.
View all literature mentionsMus musculus with name C57BL/6J from IMSR.
View all literature mentionsAllele Detail: Targeted This is a legacy resource.
View all literature mentionsMus musculus with name B6.Cg-Tg(TcraTcrb)425Cbn/J from IMSR.
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View all literature mentionsThis monoclonal targets Btk (pY223)/Itk (pY180)
View all literature mentionsThis monoclonal targets Akt (pS473)
View all literature mentionsThis monoclonal targets Mouse CXCR5
View all literature mentionsThis polyclonal targets gamma Tubulin antibody
View all literature mentionsThis monoclonal targets Zap70 (Y319) / Syk (Y352)
View all literature mentionsThis monoclonal targets CD45R / B220
View all literature mentionsThis monoclonal targets CD279 (PD-1)
View all literature mentionsThis monoclonal targets T- & B-Cell Activation Antigen
View all literature mentionsThis monoclonal targets CD45.2
View all literature mentionsThis monoclonal targets RAT ANTI MOUSE CD169:Alexa Fluor 647
View all literature mentionsThis monoclonal targets Lamp1
View all literature mentionsThis monoclonal targets CD4
View all literature mentionsThis unknown targets Myosin Light Chain, phospho (Ser19)
View all literature mentionsThis monoclonal targets CD95 (Fas)
View all literature mentionsThis monoclonal targets CD45R / B220
View all literature mentionsThis polyclonal targets gamma Tubulin antibody
View all literature mentionsThis monoclonal targets Lamp1
View all literature mentionsThis monoclonal targets CD45.2
View all literature mentionsThis monoclonal targets RAT ANTI MOUSE CD169:Alexa Fluor 647
View all literature mentionsThis monoclonal targets CD4
View all literature mentionsThis monoclonal targets Zap70 (Y319) / Syk (Y352)
View all literature mentionsThis unknown targets Biotin
View all literature mentionsThis monoclonal targets ERK1 / ERK2, phospho (Thr202 / Tyr204)
View all literature mentionsThis monoclonal targets CD279 (PD-1)
View all literature mentionsThis monoclonal targets T- & B-Cell Activation Antigen
View all literature mentionsThis monoclonal targets CD95 (Fas)
View all literature mentionsThis monoclonal targets Mouse CXCR5
View all literature mentionsThis monoclonal targets Akt (pS473)
View all literature mentionsThis monoclonal targets Btk (pY223)/Itk (pY180)
View all literature mentionsThis monoclonal targets Zap70 (Y319) / Syk (Y352)
View all literature mentionsThis monoclonal targets ERK1 / ERK2, phospho (Thr202 / Tyr204)
View all literature mentionsThis unknown targets Myosin Light Chain, phospho (Ser19)
View all literature mentionsThis unknown targets Biotin
View all literature mentionsThis polyclonal targets Chicken Lysozyme (Biotin)
View all literature mentionsThis monoclonal targets CD95 (Fas)
View all literature mentionsThis monoclonal targets T- & B-Cell Activation Antigen
View all literature mentionsThis monoclonal targets CD279 (PD-1)
View all literature mentionsThis monoclonal targets Mouse CXCR5
View all literature mentionsThis monoclonal targets CD45R / B220
View all literature mentionsThis monoclonal targets CD4
View all literature mentionsThis monoclonal targets CD45.2
View all literature mentionsThis monoclonal targets RAT ANTI MOUSE CD169:Alexa Fluor 647
View all literature mentionsThis monoclonal targets Lamp1
View all literature mentionsThis polyclonal targets gamma Tubulin antibody
View all literature mentionsThis monoclonal targets Akt (pS473)
View all literature mentionsThis monoclonal targets Btk (pY223)/Itk (pY180)
View all literature mentionsThis unknown targets Myosin Light Chain, phospho (Ser19)
View all literature mentionsThis polyclonal targets Chicken Lysozyme (Biotin)
View all literature mentions