In Drosophila, 23-30 nt long PIWI-interacting RNAs (piRNAs) direct the protein Piwi to silence germline transposon transcription. Most germline piRNAs derive from dual-strand piRNA clusters, heterochromatic transposon graveyards that are transcribed from both genomic strands. These piRNA sources are marked by the heterochromatin protein 1 homolog Rhino (Rhi), which facilitates their promoter-independent transcription, suppresses splicing, and inhibits transcriptional termination. Here, we report that the protein Maelstrom (Mael) represses canonical, promoter-dependent transcription in dual-strand clusters, allowing Rhi to initiate piRNA precursor transcription. Mael also represses promoter-dependent transcription at sites outside clusters. At some loci, Mael repression requires the piRNA pathway, while at others, piRNAs play no role. We propose that by repressing canonical transcription of individual transposon mRNAs, Mael helps Rhi drive non-canonical transcription of piRNA precursors without generating mRNAs encoding transposon proteins.
Pubmed ID: 30527661 RIS Download
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View all literature mentionsJava toolset for working with next generation sequencing data in the BAM format.
View all literature mentionsA generic alignment format for storing read alignments against reference sequences, supporting short and long reads (up to 128 Mbp) produced by different sequencing platforms.
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View all literature mentionsDrosophila melanogaster with name P{ry[+t7.2]=PZ}rhi[02086] cn[1]/CyO; ry[506] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; mael[r20]/TM3, Sb[1] from BDSC.
View all literature mentionsThis unknown targets IgG
View all literature mentionsThis monoclonal targets tubulin (alpha-)
View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.GL00041}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; armi[72.1]/TM6C, Sb[1] Tb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; mael[r20]/TM3, Sb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{ry[+t7.2]=ry11}piwi[2]/CyO, P{w[+mC]=ActGFP}JMR1 from BDSC.
View all literature mentionsDrosophila melanogaster with name P{w[+mC]=otu-GAL4::VP16.R}1, w[*]; P{w[+mC]=GAL4-nos.NGT}40; P{w[+mC]=GAL4::VP16-nos.UTR}CG6325[MVD1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[67c23] P{y[+mDint2] w[+mC]=EPgy2}DIP1[EY02625] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; P{w[+mC]=GAL4-nos.NGT}40 from BDSC.
View all literature mentionsDrosophila melanogaster with name In(1)w[m4] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[67c23]; P{y[+mDint2] w[+mC]=EPgy2}EY08366 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; armi[72.1]/TM6C, Sb[1] Tb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.GL00054}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.GL00041}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1]; P{y[+mDint2] w[BR.E.BR]=SUPor-P}rhi[KG00910] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{ry[+t7.2]=ry11}piwi[2]/CyO, P{w[+mC]=ActGFP}JMR1 from BDSC.
View all literature mentionsThis polyclonal targets IgG
View all literature mentionsDrosophila melanogaster with name w[*]; mael[r20]/TM3, Sb[1] from BDSC.
View all literature mentionsThis unknown targets IgG
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View all literature mentionsDrosophila melanogaster with name P{ry[+t7.2]=PZ}rhi[02086] cn[1]/CyO; ry[506] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[67c23] P{y[+mDint2] w[+mC]=EPgy2}DIP1[EY02625] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; P{w[+mC]=GAL4-nos.NGT}40 from BDSC.
View all literature mentionsDrosophila melanogaster with name In(1)w[m4] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[67c23]; P{y[+mDint2] w[+mC]=EPgy2}EY08366 from BDSC.
View all literature mentionsDrosophila melanogaster with name P{w[+mC]=otu-GAL4::VP16.R}1, w[*]; P{w[+mC]=GAL4-nos.NGT}40; P{w[+mC]=GAL4::VP16-nos.UTR}CG6325[MVD1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1]; P{y[+mDint2] w[BR.E.BR]=SUPor-P}rhi[KG00910] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.GL00054}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name P{ry[+t7.2]=PZ}rhi[02086] cn[1]/CyO; ry[506] from BDSC.
View all literature mentions