Class III Semaphorin (Sema) secreted ligands are known to repel neurites expressing Neuropilin (Nrp) and/or Plexin (Plxn) receptors. There is, however, a growing body of literature supporting that Sema signaling also has alternative roles in development such as synaptogenesis, boundary formation, and vasculogenesis. To evaluate these options during inner ear development, we used in situ hybridization or immunohistochemistry to map the expression of Sema3D, Sema3F, Nrp1, Nrp2, and PlxnA1 in the chicken (Gallus gallus) inner ear from embryonic day (E)5-E10. The resulting expression patterns in either the otic epithelium or its surrounding mesenchyme suggest that Sema signaling could be involved in each of the varied functions reported for other tissues. Sema3D expression flanking the sensory tissue in vestibular organs suggests that it may repel Nrp2- and PlxnA1-expressing neurites of the vestibular ganglion away from nonsensory epithelia, thus channeling them into the sensory domains at E5-E8. Expression of Sema signaling genes in the sensory hair cells of both the auditory and vestibular organs on E8-E10 may implicate Sema signaling in synaptogenesis. In the nonsensory regions of the cochlea, Sema3D in the future tegmentum vasculosum opposes Nrp1 and PlxnA1 in the future cuboidal cells; the abutment of ligand and receptors in adjacent domains may enforce or maintain the boundary between them. In the mesenchyme, Nrp1 colocalized with capillary-rich tissue. Sema3D immediately flanks this Nrp1-expressing tissue, suggesting a role in endothelial cell migration towards the inner ear. In summary, Sema signaling may play multiple roles in the developing inner ear.
Pubmed ID: 30520042 RIS Download
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This monoclonal targets Mouse Avian Myoblastosis Virus p19^(gag)
View all literature mentionsThis monoclonal targets neurofilament associated
View all literature mentionsThis polyclonal targets Rat Neuropilin-1
View all literature mentionsThis monoclonal targets Tubulin III, beta (neuronal)
View all literature mentionsCell line UMNSAH/DF-1 is a Spontaneously immortalized cell line with a species of origin Gallus gallus
View all literature mentionsThis monoclonal targets neurofilament associated
View all literature mentionsThis monoclonal targets Mouse Avian Myoblastosis Virus p19^(gag)
View all literature mentionsCell line UMNSAH/DF-1 is a Spontaneously immortalized cell line with a species of origin Gallus gallus
View all literature mentionsThis monoclonal targets Mouse Avian Myoblastosis Virus p19^(gag)
View all literature mentionsThis monoclonal targets Tubulin III, beta (neuronal)
View all literature mentionsThis polyclonal targets Rat Neuropilin-1
View all literature mentionsThis monoclonal targets Tubulin III, beta (neuronal)
View all literature mentionsThis polyclonal targets Rat Neuropilin-1
View all literature mentionsThis monoclonal targets Mouse Avian Myoblastosis Virus p19^(gag)
View all literature mentionsCell line UMNSAH/DF-1 is a Spontaneously immortalized cell line with a species of origin Gallus gallus
View all literature mentions