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Ubiquitin is an essential signaling protein that controls many different cellular processes. While cellular ubiquitin levels normally cycle between pools of free and conjugated ubiquitin, the balance of these ubiquitin pools can be shifted by exposure to a variety of cellular stresses. Altered ubiquitin pools are also observed in several neurological disorders, suggesting that imbalances in ubiquitin homeostasis may contribute to neuronal dysfunction. To examine the effects of increased ubiquitin levels on the mammalian nervous system, we generated transgenic mice that express ubiquitin under the control of the Thy1.2 promoter. While we did not detect global changes in levels of ubiquitin conjugates in the hippocampus, we found that increasing ubiquitin levels reduced AMPA (GRIA1-4) receptor expression without affecting the levels of NMDA (GRIN) or GABAA receptors. Ubiquitin over-expression also negatively impacted hippocampus-dependent learning and memory as well as baseline excitability and synaptic plasticity at hippocampal CA3-CA1 synapses. These changes occurred in a dose-dependent manner in that mice with the highest levels of ubiquitin over-expression had the greatest deficits in synaptic function and were the most impaired in the learning and memory tasks. As chronic elevation of ubiquitin expression in neurons is sufficient to cause changes in synaptic function and cognition, altered ubiquitin homeostasis may be an important contributor to the stress-induced changes observed in neurological disorders.
Pubmed ID: 30451289 RIS Download
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This monoclonal targets AMPA Receptor (GluR 4) (D41A11) XP Rabbit mAb
View all literature mentionsThis polyclonal targets GRIN2A
View all literature mentionsThis monoclonal targets Human Cyclin D1 MAb (Clone 328303)
View all literature mentionsThis monoclonal targets Bassoon mAb (SAP7F407)
View all literature mentionsThis monoclonal targets VPS28 (B-2)
View all literature mentionsThis monoclonal targets AMPA Receptor (GluR 3) (D25G9) Rabbit mAb
View all literature mentionsThis monoclonal targets PSD-95 MAGUK scaffolding protein
View all literature mentionsThis polyclonal targets UBE2Z
View all literature mentionsThis monoclonal targets GluA2/GluR2 glutamate receptor
View all literature mentionsThis polyclonal targets SNAP25
View all literature mentionsThis polyclonal targets NEDD4-1 (H-135)
View all literature mentionsThis monoclonal targets tubulin (alpha-)
View all literature mentionsThis polyclonal targets TSG101
View all literature mentionsThis monoclonal targets GAPDH
View all literature mentionsThis polyclonal targets Ig (H+L)
View all literature mentionsThis monoclonal targets STAM
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis monoclonal targets UCH-L5 (C-4)
View all literature mentionsThis monoclonal targets Ubiquitin
View all literature mentionsThis polyclonal targets GABA
View all literature mentionsThis polyclonal targets Synphilin-1, aa829-847 hsynclein alpha
View all literature mentionsThis polyclonal targets UBCH7
View all literature mentionsThis monoclonal targets Synaptophysin
View all literature mentionsThis polyclonal targets rabbit IgG
View all literature mentionsThis monoclonal targets Synaptophysin
View all literature mentionsThis polyclonal targets SNAP25
View all literature mentionsThis monoclonal targets GAPDH
View all literature mentionsThis monoclonal targets Ubiquitin
View all literature mentionsThis monoclonal targets PSD-95 MAGUK scaffolding protein
View all literature mentionsThis polyclonal targets rabbit IgG
View all literature mentionsThis monoclonal targets Bassoon mAb (SAP7F407)
View all literature mentionsThis polyclonal targets Synphilin-1, aa829-847 hsynclein alpha
View all literature mentionsThis monoclonal targets Synaptophysin
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis monoclonal targets tubulin (alpha-)
View all literature mentionsThis polyclonal targets Ig (H+L)
View all literature mentionsThis monoclonal targets AMPA Receptor (GluR 4) (D41A11) XP Rabbit mAb
View all literature mentionsThis monoclonal targets AMPA Receptor (GluR 3) (D25G9) Rabbit mAb
View all literature mentionsThis monoclonal targets GluA2/GluR2 glutamate receptor
View all literature mentionsThis monoclonal targets VPS28 (B-2)
View all literature mentionsThis monoclonal targets STAM
View all literature mentionsThis polyclonal targets TSG101
View all literature mentionsThis monoclonal targets Synaptophysin
View all literature mentionsThis monoclonal targets UCH-L5 (C-4)
View all literature mentionsThis polyclonal targets GRIN2A
View all literature mentionsThis monoclonal targets Human Cyclin D1 MAb (Clone 328303)
View all literature mentionsThis polyclonal targets NEDD4-1 (H-135)
View all literature mentionsThis polyclonal targets GABA
View all literature mentionsThis polyclonal targets UBE2Z
View all literature mentionsThis polyclonal targets UBCH7
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