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The mitochondrial respiratory chain is organized in a dynamic set of supercomplexes (SCs). The COX7A2L protein is essential for mammalian SC III2+IV assembly. However, its function in respirasome (SCs I+III2+IVn) biogenesis remains controversial. To unambiguously determine the COX7A2L role, we generated COX7A2L-knockout (COX7A2L-KO) HEK293T and U87 cells. COX7A2L-KO cells lack SC III2+IV but have enhanced complex III steady-state levels, activity, and assembly rate, normal de novo complex IV biogenesis, and delayed respirasome formation. Nonetheless, the KOs have normal respirasome steady-state levels, and only larger structures (SCs I1-2+III2+IV2-n or megacomplexes) were undetected. Functional substrate-driven competition assays showed normal mitochondrial respiration in COX7A2L-KO cells in standard and nutritional-, environmental-, and oxidative-stress-challenging conditions. We conclude that COX7A2L establishes a regulatory checkpoint for the biogenesis of CIII2 and specific SCs, but the COX7A2L-dependent MRC remodeling is essential neither to maintain mitochondrial bioenergetics nor to cope with acute cellular stresses.
Pubmed ID: 30428348 RIS Download
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View all literature mentionsCommercial organization which provides reagents and services for molecular biology research. Its services include clone collections, microRNA solutions, genome editing, qPCR products, and fluorescent labeling and detection.
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View all literature mentionsAn Antibody supplier and subset of ThermoFisher Scientific which provides fluorescence reagents for various experiments and methods.
View all literature mentionsCell line HEK293T is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line HEK293 is a Transformed cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line U-87MG ATCC is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsThis monoclonal targets Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5]
View all literature mentionsThis polyclonal targets Tom20 (FL-145)
View all literature mentionsThis polyclonal targets COX4I2
View all literature mentionsThis polyclonal targets BCS1L antibody
View all literature mentionsThis monoclonal targets TIMM50 antibody [EPR5784]
View all literature mentionsThis polyclonal targets COX6A1
View all literature mentionsThis polyclonal targets COX6A2 antibody
View all literature mentionsThis monoclonal targets ATP5A
View all literature mentionsThis polyclonal targets SOD2 antibody produced in rabbit
View all literature mentionsThis polyclonal targets COX7A2L
View all literature mentionsThis monoclonal targets SDHA
View all literature mentionsThis monoclonal targets Uqcrc2
View all literature mentionsThis monoclonal targets HSPA6
View all literature mentionsThis polyclonal targets Ndufs1 antibody
View all literature mentionsThis monoclonal targets Cytochrome C oxidase subunit II antibody [12C4F12 ]
View all literature mentionsThis monoclonal targets COX IV
View all literature mentionsThis unknown targets MOUSE IgG (H&L) Antibody Peroxidase Conjugated Pre-adsorbed
View all literature mentionsThis monoclonal targets FLAG
View all literature mentionsThis monoclonal targets NDUFA9
View all literature mentionsThis monoclonal targets beta-Tubulin-Cy3 antibody produced in mouse
View all literature mentionsThis unknown targets RABBIT IgG (H&L) Secondary Antibody Peroxidase Conjugated
View all literature mentionsThis polyclonal targets RCJMB04_4h19
View all literature mentionsThis monoclonal targets VDAC1 / Porin
View all literature mentionsThis monoclonal targets COX5b (B-2)
View all literature mentionsThis polyclonal targets COX7A2L
View all literature mentionsThis polyclonal targets Tom20 (FL-145)
View all literature mentionsThis monoclonal targets VDAC1 / Porin
View all literature mentionsThis unknown targets RABBIT IgG (H&L) Secondary Antibody Peroxidase Conjugated
View all literature mentionsThis monoclonal targets ATP5A
View all literature mentionsThis monoclonal targets Uqcrc2
View all literature mentionsThis polyclonal targets BCS1L antibody
View all literature mentionsThis monoclonal targets Cytochrome C oxidase subunit II antibody [12C4F12 ]
View all literature mentionsThis monoclonal targets Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5]
View all literature mentionsThis monoclonal targets COX IV
View all literature mentionsThis polyclonal targets COX6A1
View all literature mentionsThis polyclonal targets RCJMB04_4h19
View all literature mentionsThis unknown targets MOUSE IgG (H&L) Antibody Peroxidase Conjugated Pre-adsorbed
View all literature mentionsThis polyclonal targets SOD2 antibody produced in rabbit
View all literature mentionsThis monoclonal targets FLAG
View all literature mentionsThis monoclonal targets NDUFA9
View all literature mentionsThis monoclonal targets HSPA6
View all literature mentionsThis monoclonal targets SDHA
View all literature mentionsThis monoclonal targets beta-Tubulin-Cy3 antibody produced in mouse
View all literature mentionsThis polyclonal targets COX4I2
View all literature mentionsThis polyclonal targets COX6A2 antibody
View all literature mentionsThis monoclonal targets TIMM50 antibody [EPR5784]
View all literature mentionsThis monoclonal targets COX5b (B-2)
View all literature mentionsThis polyclonal targets Ndufs1 antibody
View all literature mentionsThis monoclonal targets beta-Tubulin-Cy3 antibody produced in mouse
View all literature mentionsThis monoclonal targets COX IV
View all literature mentionsThis monoclonal targets Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5]
View all literature mentionsThis polyclonal targets RCJMB04_4h19
View all literature mentionsThis unknown targets RABBIT IgG (H&L) Secondary Antibody Peroxidase Conjugated
View all literature mentionsThis unknown targets MOUSE IgG (H&L) Antibody Peroxidase Conjugated Pre-adsorbed
View all literature mentionsThis polyclonal targets SOD2 antibody produced in rabbit
View all literature mentionsThis monoclonal targets VDAC1 / Porin
View all literature mentionsThis monoclonal targets NDUFA9
View all literature mentionsThis polyclonal targets Tom20 (FL-145)
View all literature mentionsThis monoclonal targets TIMM50 antibody [EPR5784]
View all literature mentionsThis monoclonal targets SDHA
View all literature mentionsThis polyclonal targets Ndufs1 antibody
View all literature mentionsThis monoclonal targets FLAG
View all literature mentionsThis monoclonal targets HSPA6
View all literature mentionsThis polyclonal targets COX6A1
View all literature mentionsThis polyclonal targets COX7A2L
View all literature mentionsThis polyclonal targets COX6A2 antibody
View all literature mentionsThis monoclonal targets COX5b (B-2)
View all literature mentionsThis monoclonal targets Cytochrome C oxidase subunit II antibody [12C4F12 ]
View all literature mentionsThis polyclonal targets COX4I2
View all literature mentionsThis monoclonal targets Uqcrc2
View all literature mentionsThis polyclonal targets BCS1L antibody
View all literature mentionsThis monoclonal targets ATP5A
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