Understanding general principles that safeguard cellular identity should reveal critical insights into common mechanisms underlying specification of varied cell types. Here, we show that SUMO modification acts to stabilize cell fate in a variety of contexts. Hyposumoylation enhances pluripotency reprogramming in vitro and in vivo, increases lineage transdifferentiation, and facilitates leukemic cell differentiation. Suppressing sumoylation in embryonic stem cells (ESCs) promotes their conversion into 2-cell-embryo-like (2C-like) cells. During reprogramming to pluripotency, SUMO functions on fibroblastic enhancers to retain somatic transcription factors together with Oct4, Sox2, and Klf4, thus impeding somatic enhancer inactivation. In contrast, in ESCs, SUMO functions on heterochromatin to silence the 2C program, maintaining both proper H3K9me3 levels genome-wide and repression of the Dux locus by triggering recruitment of the sumoylated PRC1.6 and Kap/Setdb1 repressive complexes. Together, these studies show that SUMO acts on chromatin as a glue to stabilize key determinants of somatic and pluripotent states.
Pubmed ID: 30401455 RIS Download
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Computational biology resource for investigating candidate functional sites in eukarytic proteins. Functional sites which fit to the description linear motif are currently specified as patterns using Regular Expression rules. To improve the predictive power, context-based rules and logical filters are being developed and applied to reduce the amount of false positives. The current version of the ELM server provides core functionality including filtering by cell compartment, phylogeny, globular domain clash (using the SMART/Pfam databases) and structure. In addition, both the known ELM instances and any positionally conserved matches in sequences similar to ELM instance sequences are identified and displayed (see ELM instance mapper). Although the ELM resource contains a large collection of functional site motifs, the current set of motifs is not exhaustive.
View all literature mentionsQuality control software that perform checks on raw sequence data coming from high throughput sequencing pipelines. This software also provides a modular set of analyses which can give a quick impression of the quality of the data prior to further analysis.
View all literature mentionsThis monoclonal targets Histone H2A (Lys119)
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View all literature mentionsThis monoclonal targets UBE2I / UBC9 antibody [EP2938Y] - ChIP Grade
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View all literature mentionsThis polyclonal targets POU5F1
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View all literature mentionsThis polyclonal targets H3K27ac
View all literature mentionsThis isotype control targets not applicable
View all literature mentionsThis monoclonal targets CEBP Beta
View all literature mentionsThis polyclonal targets L3MBTL2
View all literature mentionsThis monoclonal targets Tubulin ? 3 (TUBB3)
View all literature mentionsThis monoclonal targets Sumo 1
View all literature mentionsThis monoclonal targets Human Sumo 2 / 3
View all literature mentionsThis monoclonal targets Human CD11b
View all literature mentionsThis recombinant monoclonal targets CD11b
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View all literature mentionsCell line HL-60 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsSoftware performing alignment of high-throughput RNA-seq data. Aligns RNA-seq reads to reference genome using uncompressed suffix arrays.
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View all literature mentionsSoftware for single-cell flow cytometry analysis. Its functions include management, display, manipulation, analysis and publication of the data stream produced by flow and mass cytometers.
View all literature mentionsA high-performance visualization tool for interactive exploration of large, integrated genomic datasets.
View all literature mentionsSoftware package for differential gene expression analysis based on the negative binomial distribution. Used for analyzing RNA-seq data for differential analysis of count data, using shrinkage estimation for dispersions and fold changes to improve stability and interpretability of estimates.
View all literature mentionsSoftware performing alignment of high-throughput RNA-seq data. Aligns RNA-seq reads to reference genome using uncompressed suffix arrays.
View all literature mentionsStatistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsUltrafast and memory efficient tool for aligning sequencing reads to long reference sequences. Supports gapped, local, and paired end alignment modes. More suited to finding longer, gapped alignments in comparison with original Bowtie method.
View all literature mentionsSoftware Python package for identifying transcript factor binding sites. Used to evaluate significance of enriched ChIP regions. Improves spatial resolution of binding sites through combining information of both sequencing tag position and orientation. Can be used for ChIP-Seq data alone, or with control sample with increase of specificity.
View all literature mentionsSoftware for single-cell flow cytometry analysis. Its functions include management, display, manipulation, analysis and publication of the data stream produced by flow and mass cytometers.
View all literature mentionsOpen source Java based image processing software program designed for scientific multidimensional images. ImageJ has been transformed to ImageJ2 application to improve data engine to be sufficient to analyze modern datasets.
View all literature mentionsThis monoclonal targets CEBP Beta
View all literature mentionsThis monoclonal targets Histone H3 antibody [mAbcam 24834]
View all literature mentionsThis isotype control targets not applicable
View all literature mentionsThis polyclonal targets H3K27ac
View all literature mentionsThis monoclonal targets ChIPAb+ Trimethyl-Histone H3 (Lys4)
View all literature mentionsThis polyclonal targets PCGF6
View all literature mentionsThis polyclonal targets L3MBTL2
View all literature mentionsThis polyclonal targets DEDAF antibody produced in rabbit
View all literature mentionsThis polyclonal targets C/EBP alpha
View all literature mentionsThis monoclonal targets Human CD11b
View all literature mentionsThis polyclonal targets POU5F1
View all literature mentionsThis monoclonal targets UBE2I / UBC9 antibody [EP2938Y] - ChIP Grade
View all literature mentionsThis monoclonal targets Sumo 1
View all literature mentionsThis polyclonal targets RING2 / RING1B / RNF2 antibody
View all literature mentionsThis monoclonal targets Tubulin ? 3 (TUBB3)
View all literature mentionsThis monoclonal targets Human Sumo 2 / 3
View all literature mentionsCell line HL-60 is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsThis monoclonal targets Histone H2A (Lys119)
View all literature mentionsThis polyclonal targets SETDB1
View all literature mentionsThis monoclonal targets β-Actin
View all literature mentionsThis recombinant monoclonal targets CD11b
View all literature mentionsThis polyclonal targets Histone H3, trimethyl (Lys27)
View all literature mentionsThis polyclonal targets H3K9me3
View all literature mentionsThis polyclonal targets H3K4me1
View all literature mentions