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Amino acid absorption requires luminal uptake into and subsequent basolateral efflux out of epithelial cells, with the latter step being critical to regulate the intracellular concentration of the amino acids. The basolateral essential neutral amino acid uniporter LAT4 (SLC43A2) has been suggested to drive the net efflux of non-essential and cationic amino acids via parallel amino acid antiporters by recycling some of their substrates; its deletion has been shown to cause defective postnatal growth and death in mice. Here we test the regulatory function of LAT4 phosphorylation sites by mimicking their phosphorylated and dephosphorylated states in Xenopus laevis oocytes and show that dephosphorylation of S274 and phosphorylation of S297 increase LAT4 membrane localization and function. Using new phosphorylation site-specific antibodies, we observe changes in LAT4 phosphorylation in mouse small intestine that correspond to its upregulation at the expected feeding time. These results strongly suggest that LAT4 phosphorylation participates in the regulation of transepithelial amino acid absorption.
Pubmed ID: 30379325 RIS Download
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This unknown targets
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View all literature mentionsMus musculus with name B6.129-Gt(ROSA)26Sortm1(cre/ERT2)Tyj/J from IMSR.
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View all literature mentionsMicroscope that can visualize multiple nuclear, cytoplasmic and membrane markers in the same tissue section and with intact morphology. Its features include detection and measurement of weakly expressed and overlapping biomarkers in fixed tissue sections, autofluorescence removal technology, and the ability to work with both fluorescence and brightfield samples.
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View all literature mentionsTHIS RESOURCE IS NO LONGER IN SERVICE. Documented on September 23,2022. 02111-1307, USA. Sponsors: ImageJ Mosaic Plug-Ins software presented here was produced at the National Center for Microscopy and Imaging Research at San Diego, which is supported by the National Institutes of Health (NIH) through a National Center for Research Resources program grant P41 RR04050.
View all literature mentionsThis monoclonal targets β-Actin
View all literature mentionsMus musculus with name B6.129-Gt(ROSA)26Sortm1(cre/ERT2)Tyj/J from IMSR.
View all literature mentionsThis polyclonal targets FLAG
View all literature mentionsThis monoclonal targets Human Tubulin, beta
View all literature mentionsSoftware for automatic general image analysis. It provides fully automatic analysis of 1-D gels including lane creation, background subtraction, band detection, molecular weight calibration, quantity calibration, and normalization. Editing tools are provided for cropping, rotating, and filtering images.
View all literature mentionsThis unknown targets Mouse IgG (H+L) AP Conjugate
View all literature mentionsThis polyclonal targets Rabbit IgG H&L
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal targets Donkey anti-Rabbit IgG H&L (DyLight® 594) secondary antibody
View all literature mentionsThis monoclonal targets E-CADHERIN
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsStatistical analysis software that combines scientific graphing, comprehensive curve fitting (nonlinear regression), understandable statistics, and data organization. Designed for biological research applications in pharmacology, physiology, and other biological fields for data analysis, hypothesis testing, and modeling.
View all literature mentionsThis unknown targets
View all literature mentionsMicroscope that can visualize multiple nuclear, cytoplasmic and membrane markers in the same tissue section and with intact morphology. Its features include detection and measurement of weakly expressed and overlapping biomarkers in fixed tissue sections, autofluorescence removal technology, and the ability to work with both fluorescence and brightfield samples.
View all literature mentionsThe SciCrunch Infrastructure was developed as a cooperative data platform to be used by diverse communities in making data more FAIR.
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