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Bone cells sense and actively adapt to physical perturbations to prevent critical damage. ATP release is among the earliest cellular responses to mechanical stimulation. Mechanical stimulation of a single murine osteoblast led to the release of 70 ± 24 amole ATP, which stimulated calcium responses in neighboring cells. Osteoblasts contained ATP-rich vesicles that were released upon mechanical stimulation. Surprisingly, interventions that promoted vesicular release reduced ATP release, while inhibitors of vesicular release potentiated ATP release. Searching for an alternative ATP release route, we found that mechanical stresses induced reversible cell membrane injury in vitro and in vivo. Ca2+/PLC/PKC-dependent vesicular exocytosis facilitated membrane repair, thereby minimizing cell injury and reducing ATP release. Priming cellular repair machinery prior to mechanical stimulation reduced subsequent membrane injury and ATP release, linking cellular mechanosensitivity to prior mechanical exposure. Thus, our findings position ATP release as an integrated readout of membrane injury and repair.
Pubmed ID: 30324907 RIS Download
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Cell line C2C12 is a Spontaneously immortalized cell line with a species of origin Mus musculus (Mouse)
View all literature mentionsMus musculus with name C57BL/6J from IMSR.
View all literature mentionsThis unknown targets Phospho-PKC Sampler Kit
View all literature mentionsThis monoclonal targets PKCdelta (D10E2) Rabbit mAb
View all literature mentionsThis monoclonal targets beta-Tubulin
View all literature mentionsThis monoclonal targets GAPDH
View all literature mentionsThis polyclonal secondary targets IgG
View all literature mentionsThis unknown targets
View all literature mentionsThis monoclonal targets PKCzeta
View all literature mentionsThis polyclonal targets Mouse SOST
View all literature mentionsThis polyclonal targets Goat IgG
View all literature mentionsMus musculus with name SEA/GnJ from IMSR.
View all literature mentionsThis monoclonal targets GAPDH
View all literature mentionsThis monoclonal targets PKCdelta (D10E2) Rabbit mAb
View all literature mentionsMus musculus with name SEA/GnJ from IMSR.
View all literature mentionsThis unknown targets
View all literature mentionsThis polyclonal secondary targets IgG
View all literature mentionsThis polyclonal targets Goat IgG
View all literature mentionsThis unknown targets Phospho-PKC Sampler Kit
View all literature mentionsThis monoclonal targets beta-Tubulin
View all literature mentionsThis polyclonal targets Mouse SOST
View all literature mentionsThis monoclonal targets PKCzeta
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