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The non-hematopoietic cell fraction of the bone marrow (BM) is classically identified as CD45- Ter119- CD31- (herein referred to as triple-negative cells or TNCs). Although TNCs are believed to contain heterogeneous stromal cell populations, they remain poorly defined. Here we showed that the vast majority of TNCs (∼85%) have a hematopoietic rather than mesenchymal origin. Single cell RNA-sequencing revealed erythroid and lymphoid progenitor signatures among CD51- TNCs. Ly6D+ CD44+ CD51- TNCs phenotypically and functionally resembled CD45+ pro-B lymphoid cells, whereas Ly6D- CD44+ CD51- TNCs were enriched in previously unappreciated stromal-dependent erythroid progenitors hierarchically situated between preCFU-E and proerythroblasts. Upon adoptive transfer, CD44+ CD51- TNCs contributed to repopulate the B-lymphoid and erythroid compartments. CD44+ CD51- TNCs also expanded during phenylhydrazine-induced acute hemolysis or in a model of sickle cell anemia. These findings thus uncover physiologically relevant new classes of stromal-associated functional CD45- hematopoietic progenitors.
Pubmed ID: 30314756 RIS Download
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An independent, nonprofit organization focused on mammalian genetics research to advance human health. Their mission is to discover the genetic basis for preventing, treating, and curing human disease, and to enable research for the global biomedical community. Jackson Laboratory breeds and manages colonies of mice as resources for other research institutions and laboratories, along with providing software and techniques. Jackson Lab also conducts genetic research and provides educational material for various educational levels.
View all literature mentionsAlgorithm for identification of rare and abundant cell types from single cell transcriptome data. Based on transcript counts obtained with unique molecular identifies. Used for discovering rare cell types and corresponding marker genes in healthy and diseased organs. Operating system Unix/Linux, Mac OS, Windows.
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View all literature mentionsThis monoclonal targets Ly-6G/Ly-6C
View all literature mentionsThis monoclonal targets CD11b
View all literature mentionsThis monoclonal targets TER-119
View all literature mentionsThis monoclonal targets CD45
View all literature mentionsThis monoclonal targets CD144
View all literature mentionsThis polyclonal targets Mouse Leptin R
View all literature mentionsThis monoclonal targets CD31
View all literature mentionsThis monoclonal targets Ly-6C
View all literature mentionsThis monoclonal targets CD41a
View all literature mentionsThis monoclonal targets CD11b
View all literature mentionsThis monoclonal targets CD90.1 (Thy-1.1)
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View all literature mentionsThis monoclonal targets IgM
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View all literature mentionsThis monoclonal targets CD150
View all literature mentionsThis monoclonal targets CD71 (Transferrin Receptor)
View all literature mentionsThis monoclonal targets CD51 (Integrin alpha V)
View all literature mentionsThis monoclonal targets CD140a (PDGFRA)
View all literature mentionsThis monoclonal targets CD127
View all literature mentionsThis monoclonal targets CD19
View all literature mentionsThis monoclonal targets Ly-6D
View all literature mentionsThis monoclonal targets CD8a
View all literature mentionsThis monoclonal targets CD4
View all literature mentionsThis monoclonal targets Ly-6G/Ly-6C
View all literature mentionsThis monoclonal targets CD11b
View all literature mentionsThis monoclonal targets CD45R (B220)
View all literature mentionsThis monoclonal targets CD135 (Flt3)
View all literature mentionsThis monoclonal targets CD34
View all literature mentionsThis monoclonal targets CD48
View all literature mentionsThis monoclonal targets CD150
View all literature mentionsThis monoclonal targets CD105
View all literature mentionsThis monoclonal targets CD16/CD32
View all literature mentionsThis monoclonal targets CD41a
View all literature mentionsThis monoclonal targets Ly-6A/E (Sca-1)
View all literature mentionsThis monoclonal targets CD117
View all literature mentionsThis monoclonal targets CD44
View all literature mentionsThis unknown targets Biotin
View all literature mentionsThis monoclonal targets CD51 (Integrin alpha V)
View all literature mentionsThis monoclonal targets CD44
View all literature mentionsThis monoclonal targets CD31
View all literature mentionsThis monoclonal targets TER-119
View all literature mentionsThis monoclonal targets CD45
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Mus musculus with name STOCK Hbatm1Paz Hbb
Software environment and programming language for statistical computing and graphics. R is integrated suite of software facilities for data manipulation, calculation and graphical display. Can be extended via packages. Some packages are supplied with the R distribution and more are available through CRAN family.It compiles and runs on wide variety of UNIX platforms, Windows and MacOS.
View all literature mentionsMus musculus with name C57BL/6J from IMSR.
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Mus musculus with name B6.SJL-Ptprca Pepc
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Mus musculus with name STOCK Hbatm1Paz Hbb
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