The preservation of germ cell sexual identity is essential for gametogenesis. Here we show that H3K9me3-mediated gene silencing is integral to female fate maintenance in Drosophila germ cells. Germ cell specific loss of the H3K9me3 pathway members, the H3K9 methyltransferase SETDB1, WDE, and HP1a, leads to ectopic expression of genes, many of which are normally expressed in testis. SETDB1 controls the accumulation of H3K9me3 over a subset of these genes without spreading into neighboring loci. At phf7, a regulator of male germ cell sexual fate, the H3K9me3 peak falls over the silenced testis-specific transcription start site. Furthermore, H3K9me3 recruitment to phf7 and repression of testis-specific transcription is dependent on the female sex determination gene Sxl. Thus, female identity is secured by an H3K9me3 epigenetic pathway in which Sxl is the upstream female-specific regulator, SETDB1 is the required chromatin writer, and phf7 is one of the critical SETDB1 target genes.
Pubmed ID: 30297796 RIS Download
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Software that estimates expression at transcript-level resolution and controls for variability evident across replicate libraries.
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View all literature mentionsFunctional genomics data repository supporting MIAME-compliant data submissions. Includes microarray-based experiments measuring the abundance of mRNA, genomic DNA, and protein molecules, as well as non-array-based technologies such as serial analysis of gene expression (SAGE) and mass spectrometry proteomic technology. Array- and sequence-based data are accepted. Collection of curated gene expression DataSets, as well as original Series and Platform records. The database can be searched using keywords, organism, DataSet type and authors. DataSet records contain additional resources including cluster tools and differential expression queries.
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View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS00205}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[1118] snf[148] P{ry[+t7.2]=neoFRT}19A/FM6 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{w[+mC]=GAL4::VP16-nos.UTR}CG6325[MVD1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.GL00531}attP40 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS00112}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS00205}attP2 from BDSC.
View all literature mentionsThis polyclonal targets H3K9me3
View all literature mentionsThis monoclonal secondary targets IgG
View all literature mentionsThis polyclonal secondary targets IgG
View all literature mentionsThis unknown targets Mouse tubulin (alpha-)
View all literature mentionsThis unknown targets Rat Drosophila vasa
View all literature mentionsThis monoclonal targets sex-lethal protein
View all literature mentionsThis polyclonal targets H3K9me3
View all literature mentionsThis monoclonal targets Spectrin, alpha
View all literature mentionsDrosophila melanogaster with name y[1] w[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[1118] snf[148] P{ry[+t7.2]=neoFRT}19A/FM6 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{w[+mC]=GAL4::VP16-nos.UTR}CG6325[MVD1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.GL00531}attP40 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS00112}attP2 from BDSC.
View all literature mentionsThis monoclonal targets HA
View all literature mentionsThis monoclonal targets HA
View all literature mentions