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Publication

Generation of two induced pluripotent stem cells lines from Mucopolysaccharydosis IIIA patient: IMEDEAi004-A and IMEDEAi004-B.

Stem cell research | 2018

Mucoplysaccharydosis IIIA (MPSIIIA) is the most severe form of Sanfilippo syndrome. Skin fibroblasts from a MPSIIIA compound heterozygous (E447K/R245H) patient were nucleofected with four OriP/EBNA1-based episomal plasmids containing: OCT3/4, SOX2, KLF4, L-Myc, LIN28, BCL-xL and shp53. The two iPSCs lines generated carry both sulfamidase enzyme (SGSH) mutations, are free of plasmid integration, have normal karyotype, express pluripotency-associated markers and are able to differentiate into the three germ layers.

Pubmed ID: 30269021 RIS Download

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

This is a list of tools and resources that we have found mentioned in this publication.

Mouse Anti-Human Sox2 Monoclonal antibody, Unconjugated, Clone 245610 (antibody)

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Goat Anti-Human Sox17 Polyclonal antibody, Unconjugated (antibody)

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Mouse IgM, k Isotype Control, Alexa Fluor 647 Conjugated, Clone G155-228 (antibody)

RRID:AB_2034030

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TRA-1-81 Antigen (antibody)

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Donkey Anti-Rat IgG (H+L) Antibody, Alexa Fluor ?? 488 Conjugated (antibody)

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This unknown targets Rat IgG (H+L)

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Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (antibody)

RRID:AB_141607

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Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 555 (antibody)

RRID:AB_162543

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Alpha-1-Fetoprotein Pathology Antibody (antibody)

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Mouse Anti-Bovine alpha-Actinin Monoclonal Antibody, Unconjugated, Clone BM-75.2 (antibody)

RRID:AB_476737

This monoclonal targets Actinin, alpha

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GATA-4 (G-4) (antibody)

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This monoclonal targets GATA-4 (G-4)

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Rabbit Anti-Neuronal Class III beta-Tubulin Purified Monoclonal Antibody, Unconjugated, Clone TUJ1 (antibody)

RRID:AB_663339

This monoclonal targets Neuronal Class III beta-Tubulin

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Anti-Nestin antibody produced in rabbit (antibody)

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This unknown targets Nestin

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Anti-TRA-1-60, clone TRA-1-60 (antibody)

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This monoclonal targets HSP90B1

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Anti-Stage-Specific Embryonic Antigen-4 (antibody)

RRID:AB_177629

This monoclonal targets Stage-Specific Embryonic Antigen-4

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Nanog (D73G4) XP Rabbit Antibody (antibody)

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This monoclonal targets Nanog

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Oct3/4 Antibody (C-10) (antibody)

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This monoclonal targets Oct-3/4

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