Homeostasis in adult tissues depends on the precise regulation of stem cells and their surrounding microenvironments, or niches. Here, we show that the cell cycle inhibitor and tumor suppressor Retinoblastoma (RB) is a critical regulator of niche cells in the Drosophila testis. The testis contains a single niche, composed of somatic hub cells, that signals to adjacent germline and somatic stem cells. Hub cells are normally quiescent, but knockdown of the RB homolog Rbf in these cells causes them to proliferate and convert to somatic stem cells. Over time, mutant hub cell clusters enlarge and split apart, forming ectopic hubs surrounded by active stem cells. Furthermore, we show that Rbf's ability to restrict niche number depends on the transcription factors E2F and Escargot and the adhesion molecule E-cadherin. Together this work reveals how precise modulation of niche cells, not only the stem cells they support, can drive regeneration and disease.
Pubmed ID: 30257208 RIS Download
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Software package as distribution of ImageJ and ImageJ2 together with Java, Java3D and plugins organized into coherent menu structure. Used to assist research in life sciences.
View all literature mentionsImaris provides range of capabilities for working with three dimensional images. Uses flexible editing and processing functions, such as interactive surface rendering and object slicing capabilities. And output to standard TIFF, Quicktime and AVI formats. Imaris accepts virtually all image formats that are used in confocal microscopy and many of those used in wide-field image acquisition.
View all literature mentionsA commercial software package that works with Carl Zeiss microscopy; AIM 4.2 software.
View all literature mentionsTHIS RESOURCE IS NO LONGER IN SERVICE. Documented on May 5,2022.Tool that predicts interactions between transcription factors and their regulated genes from binding motifs. Understanding vertebrate development requires unraveling the cis-regulatory architecture of gene regulation. PRISM provides accurate genome-wide computational predictions of transcription factor binding sites for the human and mouse genomes, and integrates the predictions with GREAT to provide functional biological context. Together, accurate computational binding site prediction and GREAT produce for each transcription factor: 1. putative binding sites, 2. putative target genes, 3. putative biological roles of the transcription factor, and 4. putative cis-regulatory elements through which the factor regulates each target in each functional role.
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal targets IgG
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis unknown targets Guinea Pig IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis unknown targets IgG (H+L)
View all literature mentionsThis polyclonal secondary targets IgG (H+L)
View all literature mentionsThis polyclonal targets Mouse IgG (H+L)
View all literature mentionsThis polyclonal targets IgY
View all literature mentionsThis polyclonal secondary targets IgY (H+L)
View all literature mentionsThis monoclonal targets Mouse Drosophila Cut protein product
View all literature mentionsThis monoclonal targets cadherin, DE- (extracellular domain)
View all literature mentionsThis monoclonal targets Mouse Drosophila adducin-related protein
View all literature mentionsThis unknown targets Cleaved Dcp-1 (Asp216)
View all literature mentionsThis monoclonal targets Mouse Drosophila Armadillo Protein
View all literature mentionsThis monoclonal targets Mouse Drosophila Eyes Absent Protein (Eya)
View all literature mentionsThis monoclonal targets Discs large (Drosophila)
View all literature mentionsThis monoclonal targets DsRed (C-20)
View all literature mentionsThis polyclonal targets DsRed
View all literature mentionsThis polyclonal targets GFP
View all literature mentionsThis polyclonal targets Vasa (d-260)
View all literature mentionsThis monoclonal targets Phospho-Histone H3 (Ser10)
View all literature mentionsThis monoclonal targets Fasciclin III (Drosophila)
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mW.hs]=Switch2}GSG2295 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=UAS-RedStinger}4, P{w[+mC]=UAS-FLP.D}JD1, P{w[+mC]=Ubi-p63E(FRT.STOP)Stinger}9F6/CyO from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=UAS-FLP.D}JD2 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.GL01293}attP40 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.JF02718}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name P{w[+mW.hs]=GawB}E132, w[*] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS03004}attP2/TM3, Sb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; PBac{y[+mDint2] w[+mC]=20XUAS-6XGFP}VK00018/CyO, P{Wee-P.ph0}Bacc[Wee-P20] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=tubP-GAL80[ts]}2/TM2 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{w[+mC]=UAS-GFP.dsRNA.R}142 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[1118]; P{w[+mC]=UAS-GFP.dsRNA.R}142 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.GL01293}attP40 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.JF02718}attP2 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS03004}attP2/TM3, Sb[1] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mW.hs]=Switch2}GSG2295 from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=UAS-FLP.D}JD2 from BDSC.
View all literature mentionsDrosophila melanogaster with name P{w[+mW.hs]=GawB}E132, w[*] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=tubP-GAL80[ts]}2/TM2 from BDSC.
View all literature mentionsDrosophila melanogaster with name y[1] w[*]; PBac{y[+mDint2] w[+mC]=20XUAS-6XGFP}VK00018/CyO, P{Wee-P.ph0}Bacc[Wee-P20] from BDSC.
View all literature mentionsDrosophila melanogaster with name w[*]; P{w[+mC]=UAS-RedStinger}4, P{w[+mC]=UAS-FLP.D}JD1, P{w[+mC]=Ubi-p63E(FRT.STOP)Stinger}9F6/CyO from BDSC.
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