Mitochondrial dysfunction plays significant roles in the pathogenesis of Parkinson's Disease (PD). The inactivation of c-Myc, a down-stream gene of Wnt/β-catenin signaling, may contribute to the mitochondria dysfunction. Inhibition of glycogen synthase kinase 3β (GSK-3β) with Alsterpaullone (Als) can activate the down-stream events of Wnt signaling. Here, we investigated the protective roles of Als against MPP+-induced cell apoptosis in SH-SY5Y cells. The data showed that Als effectively rescued c-Myc from the MPP+-induced decline via Wnt signaling. Furthermore, Als protected SH-SY5Y cells from the MPP+-induced mitochondrial fission and cell apoptosis. However, the protective roles of Als were lost under β-catenin-deficient conditions. These findings indicate that Als, a GSK-3β inhibitor, attenuated the MPP+-induced mitochondria-dependent apoptotic via up-regulation of the Wnt signaling.
Pubmed ID: 30233322 RIS Download
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View all literature mentionsA commercial antibody supplier which supplies primary and secondary antibodies, biochemicals, proteins, peptides, lysates, immunoassays and other kits.
View all literature mentionsThis polyclonal targets CASP3
View all literature mentionsThis monoclonal targets GSK-3 beta
View all literature mentionsThis monoclonal targets GAPDH
View all literature mentionsThis monoclonal targets MYC
View all literature mentionsThis monoclonal targets PARP
View all literature mentionsThis monoclonal targets beta Catenin
View all literature mentionsCell line SH-SY5Y is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsCell line SH-SY5Y is a Cancer cell line with a species of origin Homo sapiens (Human)
View all literature mentionsThis polyclonal targets CASP3
View all literature mentionsThis monoclonal targets GSK-3 beta
View all literature mentionsThis monoclonal targets GAPDH
View all literature mentionsThis monoclonal targets MYC
View all literature mentionsThis monoclonal targets PARP
View all literature mentionsThis monoclonal targets beta Catenin
View all literature mentions